How can you study the expression of a gene?

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How can you study the expression of a gene?
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Promoter activity RNA levels Protein levels
Western blot Elisa Enzyme activty
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Proteins have complex structure
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Compare it to the structure of DNA
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What is more labile?
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What factors affect the structure of your
proteins?
What factors affect the activity of your protein?
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How do we measure total amount of proteins?
The Bradford assay, a colorimetric protein assay,
is based on an Assay absorbance shift in the
dye Coomassie when bound to arginine and
hydrophobic amino acid residues present in
protein. The anionic (bound) form of the dye
is blue and has an absorption spectrum maximum at
595 nm. The increase of absorbance at 595 nm is
proportional to the amount of bound dye, and thus
to the amount (concentration) of protein present
in the sample. (Wikipedia)  
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How do we measure enzyme activity?
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Enzyme activity assays Stop assay Continuous
assay
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Could you have differences in enzyme activity of
a particular protein but its RNA levels remain
constant? Could you have the opposite effect?
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