Na

1
Transporter of the amphetamine and Na lead to an
AMPH and Na build up
Na buildup inhibits Ca2 eflux, leading to Ca2
buildup
AMPH buildup increases intracellular pH
Ca2 buildup stimulates phospholipase C (PLC)
High intracellular pH stimulates Phospholipase A2
action
PLC produces IP3 and DAG
PLA2 produces arachidonic acid
DAG and AA both stimulate the activity of Protein
Kinase C (PKC)
PKC phosphorylates the transporter
Na
AMPH
pH 7
Ca2
Na/Ca antiporter
2
Normal Vesicular release of neurotransmitter
Extracellular
Intracellular
3
Normal Vesicular release of neurotransmitter
Extracellular
Intracellular
4
Normal Vesicular release of neurotransmitter
Extracellular
5-HT
5-HT
5-HT
5-HT
5-HT
Intracellular
5-HT
5-HT
5-HT
5-HT
5
Normal Vesicular release of neurotransmitter
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
Extracellular
Intracellular
6
Normal uptake of neurotransmitter
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
Extracellular
5-HT
Intracellular
7
Normal uptake of neurotransmitter
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
Extracellular
5-HT
Intracellular
5-HT
5-HT
5-HT
5-HT
8
Phosphorylation of Transporter creates
conformational change
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
Extracellular
Intracellular
5-HT
5-HT
5-HT
5-HT
9
Conformational change creates intracellular
binding site, favoring reverse transport
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
5-HT
Extracellular
Intracellular
5-HT
5-HT
5-HT
5-HT
5-HT