ABR

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ABR??????

• Ding-Feng Su
• ???

Shanghai, China
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Arterial baroreflex (ABR)
??ABR?????????
?????????
Baroreflex Sensitivity (BRS)
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Stroke-Prone spontaneously hypertensive rats
(SHR-SP)
infarction

hemorrhage

Male 9 M Female 13 M
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BRS is an important determinant for stroke
proportion surviving
BRSgt0.3
BRSlt0.3
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Sinoaortic denervation (SAD)
SAD sinoaortic denervation
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SAD on the hemodynamic and cerebral ischemia



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??????P??(SP-SAP)
SP-SAP

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SP-SAP on the hemodynamic and cerebral ischemia
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hemodynamic and cerebral ischemia in rats with
lower BRS

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??ABR???????????
???? ???? ???? CNS ???? SHR-SP
?????? 3?? ?????60
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Effects of CR on survival time in SHR-SP
proportion surviving
CR
AL
Age (days)
Log-Rank 67.68 Plt.0001
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At age of 12 months
Means of survival time (weeks)
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Effects of CR on BP BRS
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Effects of CR on serum lipids
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Effects of CR on apoptosis of neural and
myocardial cells
Neural cells
Myocardial cells
AL CR
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Sirtuins
Sir2-like proteins (sirtuins) are a family of
NAD-depandent deacetylases.
It is accepted that the effects of CR on lifespan
is mediated by the activation of sirtuins.
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Effects of CR on sirtuin
AL CR AL CR
AL CR
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ABR function and Situin
gene
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protein
Sirt1ß-actin
Heart
Kidney
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ABR dysfunction prevented the activation of
sirtuin by CR
Sirt1ß-actin
Brain
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heart
Sirt1ß-actin
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Kidney
Sirt1ß-actin
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CR
proportion surviving
AL
CR
proportion surviving
AL
Age (days)
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??ABR??????????
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????

• ABR???????????
• ?????ACh?
• ???a7???
• ?????
• ???????

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SAD???????????
Sham
SAD
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SAD????????????
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SAD????????????
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???????a7????

a7 actin
AchT actin


Sham SAD


Sham SAD
AchT actin
a7 actin
AchT actin



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CR?SAD??????????
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???????????

• Ketanserin (???)
• An antagonist for 5-HT2A receptor with minor
  effect on blocking a1 receptor
• Enhancing BRS in SHR with a small dose
• Acting site RVLM, but not NTS.

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Effects of ket on SBP (a single dose, ig)
?
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Effects of ket on BRS (a single dose, ig)
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?
Effects of long-term treatment with Ket on BP
BRS
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?
Effects of lifelong treatment with Ket on
survival time in SHR-SP
proportion surviving
3.0mg/kg
0.3mg/kg
Control
Log-Rank 59.13 Plt0.0001
Time (days)
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• Thank you