Title: iGEM
1iGEM Synthetic BiologyInternational
Genetically Engineered Machine Competition
SB 3.0 Zurich 6/24/07
- Randy Rettberg
- igem2007.com
- parts.mit.edu
2An Engineering Question
Can simple biological systems be built from
standard, interchangable parts and operated in
living cells? Or, is biology so complex that each
case is unique?
3Education Driving Research
4International Genetically Engineered Machine
Competition 2005
- 13 Schools
- Caltech MIT UC Berkeley
- Cambridge, UK Oklahoma UCSF
- Davidson Penn State UT Austin
- ETH, Zurich Princeton
- Harvard Toronto, Canada
- Sponsored by Microsoft/MIT iCampus
5iGEM 2006
- 40 Schools, 33 Teams
- 450 participants
- Europe, Canada, Asia, Latin America, US
- Some EU funding in place
- iGEM ambassadors for US, Asia, and Europe
- Jamboree November 4 5
- www.iGEM2006.com
- Supported by Microsoft Research and iCampus
6iGEM 2006
Ege University, Turkey
7www.iGEM2006.com
8Meet the Teams
9Valencia iGEM Site
10iGEM 2006 Results
11Sample iGEM Projects
- Berkeley Controlled Communications
- Davidson Flipping DNA
- Harvard DNA Boxes
- ETH Zurich Pattern Matching
- Ljubljana Controlling Sepsis
- Chiba Japan Light-Directed Chemotaxis
- Mississippi Hydrogen Gas Detector
- MIT Banana and Wintergreen Smells
- Edinburgh Arsenic Detector
12MIT iGEM 2006
eau de coli mit igem 2006
13The field test device
- A test tube could contain all the necessary
- components Freeze dried bacteria, growth
medium, - indicator powder, Ampicillin salt, etc
- These tubes could then be given to local
villagers to monitor their own water quality
themselves - A good alternative to the widely used Gutzeit
method
www.Macteria.co.uk
14Some Engineering Answers
Can simple biological systems be built from
standard, interchangable parts and operated in
living cells? - YES, SOMETIMES Or, is biology so
complex that each case is unique? - NOT ALWAYS
15www.igem2007.com
16Financial Resources
- Small Sample
- 20K 8
- 30K 8
- 40K 1
- 50K 4
- gt50K 2
17iGEM 2007 and Beyond
- 2008 100 Schools -gt Partition?
- Geographic Competitions
- Tracts
- Health and Medicine
- Energy and the Environment
- Parts and Devices
- Fun and Games
- Sensors
- Other
18The Impact of iGEM
- Students
- Making future synthetic biologists
- Teaching graceful competition
- Instructors
- Opportunities for junior faculty
- New programs - new ideas
- A task worth the effort
- Schools
- Synthetic biology entering curriculum
- Energize research programs
- Synthetic Biology
- Examples, parts, successes, testamonials
19Registry of Standard Biological Parts
An industry based on standard parts requires
catalogues and suppliers of those parts.
20Registry Parts Catalog
http//parts.mit.edu
21Registry Contents 7/2007
1400 Parts Available as DNA
- Promoters
- Protein Coding
- Reporters
- RNA
- Terminators
- Signaling
- Many project parts
22Constitutive Promoters - Berkeley
Part Characterization
23DNA Repository
24DNA Repository
- All parts sent to teams
- Parts are from previous teams
25Robotic Assembly
26DNA Synthesis Offer
GENEART synthetic DNA offer for iGEM teams
- All you want at 0.70 per base
- 100,000 bases total at 0.25 per base
- About 2000 bases per team for 500
- GENEART is a primary sponsor of iGEM 2007
http//www.geneart.com/index.php?id252
27New Programs
- BioBrick Part Program
- iGEM in Europe
- Registry in Europe
- New Registry Workshop
- Standards Workshop
- iGEM Steering Committee
28Standards
- Device Families
- Protein DNA logic
- Post-translational control
- RNA-based control
- Localization
- Measurement
- Input and Outputs
- (PoPS - Polymerase Per Second)
- Genetic stability
- Cross-lab consistency
- Operating Environment
- Load on the cell
- Environmental impact on performance
- Models
29Sponsors
- National Science Foundation
- SynBERC
- CSBi
- GENEART
- Microsoft Research
- Biological Engineering - MIT
30Teachers Workshops
- MIT May 26th
- China June 16-17
- Zurich June 27th
- Hands-on training
- Future instructors welcome
31How can I participate?
- Organize a team for 2008
- Volunteer for the BioBrick parts program.
- Join the Awards committee
- Be a judge
- Have your lab join the Registry
- Contribute parts to the Registry
- Use BioBrick part standards
- Help raise money for teams and ambassadors
in your area. - Join iGEM committees (see the wiki)
- Join iGEM in Europe