Team members

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Biosensor
YONSEI UNIVERSITY BIOMEDICALENGINEERING
BOIMATERIAL CLASS BIOSENSOR
Biosensor

• Team members
• Kim hyo-seon
• Son hyun-mok

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Contents

• Do you know biosensor?
• Why we need better property?
• Why important hydrogen peroxide?
• Problem of pre-precursor
• Experimental Section
• Result Discussion
• Solution
• Q A

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Biosensor

• Analytical devices incoporating a biological
  material or a biomimic, intimately associated
  with or integrated within a physicochemical
  transducer or transducing microsystem, which may
  be optical, electrochemical, thermometric,
  piezoelectric or magnetic. 

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Biosensor
Target cancer cell, virus, glucose,
cho-lesterol, hormones Sensor Matrix anti-body,
enzyme, rec-eptor Signal color, fluore-secence,
redox po-tential drop, optical, mechanical, etc
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Why do we select this theme?
ltResearch bygtfrost and sullivan World Biosensor
of Market 2005, 4
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Why do we select this theme?
ltResearch bygtfrost and sullivan World Biosensor
of Market 2005, 4
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Type of Biosensors
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Application

• Glucose monitoring in diabetes patients
• Other medical health related targets
• Environmental applications e.g. the detection of
  Pesticides and river water contaminants
• Remote sensing of airborne bacteria e.g. in
  counter-bioterrorist activities
• Detection of pathogens
• Determining levels of toxic substances before and
  after bioremediation
• Detection and determining of organophospate

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Why we need better property?

• Recently, biosensor is developing many fields
• Optical, chemical, physical, and hybrid method we
  used
• Our project used amperometric biosensor
  (electrochemical)
• In this Amperometric hydrogen peroxide bio-sensor
  need more compatible surface property

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Why hydrogen peroxide is important?

• Abroad application in many fields
• Industry, clinical control and environmental
  protection
• Their is an intermediate or a product in
  biochemical reactions catalyzed by oxidase
• Monitoring of H2O2 with a reliable, rapid and
  economic method is of great significance in bio
• H2O2 generation occurs frequently in the body
• exgt glucose O2 ---gt gluconolactone H2O2
• H2O2 ---gt O2 2H

GOX
-2e-
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Why hydrogen peroxide is important?

• Good one has applied potential and pH on the
  steady-state current
• A number of studies for carried out to improve
  the electrochemical response to H2O2
• Coating the electrode surface with substances
  that have catalytic activity
• New biomaterial can get high compatibility

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Amperometric hydrogen peroxide biosensor need
more compatible surface property

• Chromatographic, colorimetric, and photometric
  methods,
• Long measure time, expensive reagent cost
• But Electrochemical sensors offer simplicity,
  high sensitivity, and selectivity
• silica sol-gel matrix has drawn much attention
• physical rigidity, chemical inertness, high
  photochemical biodegradation, and thermal
  stability
• We need a solution to solve poor water solubility
  and biocompatibility

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Problem of pre-precursor

• Pre-precursor
• TMOS (tetramethoxysilane) or TEOS
  (tetraethoxysilane)
• Poor biocompatibility
• THEOS (terakis orthosilicates)

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Experimental Section

• Sol-gel process.
• A liquid -gt A solid phase.
• The colloidal sol -gt The gel
• The fabrication of materials with a large
  variety of properties

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Experimental Section

• Preparation of Hybrid Material(Chitosan-silica
  hybrid gel)
• Materials
• The precursor THEOS(terakis orthosilicates)
• Chitosan
• Acetic acid
• Method
• Get the chitosan solution (1.0 wt)
• Using 100mL of 0.05 mol/L acetic acid and
  chitosan
• The viscous chitosan
• Stirring the chitosan solution at room
  temperature
• Add precursor THEOS

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Experimental Section

• Preparation of Hydrogen Peroxide Biosensor
• Material
• The glass carbon electrode(GCE)
• Solution of HRP/chitosan mixture
• THEOS
• Horseradish peroxide(HRP)
• Phosphate buffer
• Method
• Blending the 1.0wt chitosan solution and 5 mg/mL
  HRP solution (21(v/v))
• Add THEOS
• Dry for 10 min at room temperature
• Immerse in phosphate buffer(pH7.4) in a
  refrigerator.

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Result Discussion

• The fabricated hydrogen peroxide biosensor
• The characteristics of good analytical
  performance, reproducibility, and storage
  stability.

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Result Discussion

• Problems
• The effects of applied potential and pH value

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Solution

• Whenever we can detect target, even if it is
  bad condition.
• Non-contacting sensor
• Problem high technology cost

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Reference

• Guan-Hai Wang, Li-Ming Zhang, Using Novel
  Polysaccharide-Silica Hybrid Material to
  Construct An Amperometric Biosensor for Hydrogen
  Peroxide, 2006
• Vadim Lvovich, Alexander Scheeline, Amperometric
  Sensors for Hydrogen Peroxide, 1997
• Disposable Biosensor Based on a Hemoglobin
  Colloidal Gold-Modified creen-Printed Electrode
  for Determination of Hydrogen Peroxide
• Xiaoxing Xu, Songqin Liu, and Huangxian Ju

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Reference

• Development of amperometric biosensor for glucose
  based on a novel attractive enzyme immobilization
  matrix Calcium carbonate nanoparticles
• Dan Shan a, Mingjuan Zhua, Huaiguo Xuea,, Serge
  Cosnier b
• Design and development of a highly stable
  hydrogen peroxide biosensor on screen printed
  carbon electrode based on horseradish peroxidase
  bound with gold nanoparticles in the matrix of
  chitosan Tanin Tangkuaram , Chatchai Ponchio,
  Thippayawadee Kangkasomboon,
• Panadda Katikawong, Waret Veerasai

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