alimemtary and urogenital protozoa

1
?????????????????????????????????????????? (alime
mtary and urogenital protozoa)
2
????????????? ciliate protozoa 1. Entamoeba
histolytica 2. Entamoeba coli 3. Balantidium
coli ????????????? (flagellate protozoa) 4.
Giardia spp. 5. Trichomonas spp. 6.
Histomonas meleagridis ????? coccidia 7.Coccidi
a of dog cat - Isospora canis
- Isospora felis 8. Avian coccidia - Eimeria
spp 9. Sarcocystis spp. 10. Toxoplasma
gondii 11. Cryptosporidium spp.
3
Entamoeba histolytica
(active trophozoite)
ingested Rbc
nucleus
nucleus
halo
small central karyosome (endosome)
food vacuole
fine peripheral chromatin
cytoplasm
food vacuole
ingested Rbc
Entamoeba histolytica (mature cyst)
granular cytoplasm
chromatoid bar
4 nuclei
cytoplasm
central karyosme
cyst wall
cyst wall
fine peripheral chromatin
chromatoid bar
4
Entamoeba coli (trophozoite)
eccentric karyosome
coarse peripheral chromatin
5
Balantidium coli (trophozoite)
micronucleus
food vacuole
macronucleus
cilia
contractile vacuole
cytostome
vacuole
cytostme
macronucleus
(kidney-shaped)
macronucleus (kidney-shaped)
6
Balantidium coli (cyst)
macronucleus
cyst wall
macronucleus
????????????????????????? ???? 40-60 ??????

???????????????????????????????
?????????????????????????? macronucleus
macronucleus
cyst wall
7
Balantidium coli
section of trophozoite in
submucosa of large intestine
vacuole
macronucleus
macronucleus
macronucleus
macronucleus
macronucleus
macronucleus
contractile vacuole
nucleus
contractie vacuole
trophozoite of B. coli
contractile vacuole
macronucleus
macronucleus
cilia
host tissue
trophozoites
macronucleus
8
Giardia spp. ???? trophozoite
???????????????????????????????????????? ????
ventral surface ??? ??? dorsal ????
??? anterior half ??????????sucking disc ????????
2 ???
?? 2 nucleus, 2 axostyle, 8 flagella (4
???)
?? comma-shaped parabasal body
??????????? median ????????
sucking disc
endosome
nucleus
parabasal body
axostyle
flagellum
???? mature cyst
mature cyst ??????????????????
(oval shape)
?? nucleus 4 ???
cyst wall
??????????????????????? cyst ????? comma-shaped
parabasal body
cyst wall
nucleus (4 ???)
axostyle
comma-shaped parabasal body
9
Giardia spp.
sucking disc
endosome (nucleus)
sucking disc
flagellum
parabasal body
axostyle
axostyle
trophozoite
mature cyst
trophozoite
10
Trichomonas spp. (trophozoite)
flagellum
nucleus
axostyle
11
Histomonas meleagridis
(amoeboid form in liver)
???????amoeboid form ???????????? round parasite
cell ?????? host cell ??? space
amoeboid form of H. meleagridis
nucleus
space ?????????? host tissue ??? parasite cell
amoeboid form
host cell (liver cell)
amoeboid form
nucleus
space
12
Isospora canis
(unsporulated oocyst)
cyst wall
sporont
sporulated oocyst
cyst wall
sporozoite
sporocyst
13
Isospora felis
unsporulated oocyst
cyst wall
sporont
sporulated oocyst
cyst wall
sporocyst
sporozoite
14
Eimeria spp. ????
oocyst with zygote
zygote
oocyst wall
nucleus of zygote
???? macrogametocyte
nucleus
pink plastic granule ?????????????????????? cyst
wall
plastic peripheral pink granules ??????????????

???????? material
????????????????????? cyst wall
15
Eimeria spp. unsporulated oocyst
unsporulated oocyst ????????? zygote ?????????
oocyst with zygote, macrogametocyte
Oocyst with zygote
Oocyst with zygote
unsporulated oocyst
macrogametocyte
16
Avian coccidia Eimeria spp.
???? schizont
schizont
merozoites in mature schizont
merozoite
schizont
???? Mature schizont
17
Toxoplasma gondii
(tachyzoite)
Tachyzoite from peritoneal cavity of mouse
crescent-shaped tachyzoite
nucleus
Thin wall of cyst
bradyzoite
pseudocyst
18
Sarcocystis spp.
sarcocystis
Mieschers tubule
septum
1 compartment
septum
bradyzoite
1 compartment
muscle
muscle
septum
mature schizont
Banana-shaped bradyzoite
peripheral region
metrocyte
Sarcocystis wall
septum
19
Cryptosporidium spp. (????oocyst)
20
?????????????????????
??????????? Cryptosporidium
spp. ???????? Acid fast staining   1.
?????????????????? ?????????????????????????????
? Formalin ethyl acetate centrifugal
sedimentation ???? Sheather centrifugal
floatation ??????????????????? ??????
adhesive ???? smear ????????????????????????????
????????????? 2. Fix ??????????????
Absolute Methanol ??? 10 ???? ????
??????????? 3. ??? Fuchsin ??????????? 2 ????
4. ???????????????? (???????????????????????)
5. ??? Washing solution 1-2 ??? ????????????
6. ???????????????????????? 7. ??? Methylene
blue ???????????? 2 ???? 8. ????????????????
????????????? ?????????????????