SUMMER STUDENT REPORT

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SUMMER STUDENT REPORT

??? National Tsing Hua University Life Science
05 Dr. Ying-Hue Lee Institute of Molecular
Biology Academia Sinica R.O.C Date2003/7/18/29
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• Project IMouse behavior
• Project IIMouse brain development

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Project I mouse behavior

• Purpose find special mutants
• MaterialF3 EMS mouse
• Selection type
• Rota rod
• Hole board
• Open-field
• Water maze
• Result

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rotarod

• Major select mutants by its
• balance ability.
• We control rotating speed and acceleration time

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Data analysis
Trail A 1 to 20 rpm /60sec
Average18.29rpm 16 mice up to 15rpm
Its a easy job for mice. Still we can select the
worst in balance ability.
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Data analysis
Trail B 1 to 20 rpm/ 1sec
High-speed motor coordination
The curve represent the division into two
opposing extremes ?success or failure
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Data analysis
Trail C 1 to 60 rpm/60sec Select the best
and the worst
Approach normal distribution.
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Rotarod result
back
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Hole board

• A board has 4 X 4 holes arranged square in a
  plane
• Making the lighting dull enable the mice to
  take a
• look into the hole,and dark light make the
  mice
• Comfortable and in a leisurely manner.
• We select by it curiosity and activity.

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Data analysis
The two trail correlation coefficient0.405 The
data we obtain regard as activity reference. This
result also could be compared with open-field It
should be more trails.
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Some doubtful mice!!
10496 have lowest frequency to look into the
hole Almost he just pass the hole and look the
world outside If take off the plastic cover ,his
activity raise a little Besides, 10504 10507
dont look into the hole it seems that they have
no interest or they look clearly
back
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Open-field
As a mice,what would you do in a open field?
1.Run along two sides 2.Diagonal direction
WT
Find the exit
walk around and try to find out in the center
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keeping looking for,try to jump out or give up
Lose his head. again and repeat many times
WT

• Select by its courage

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Data analysis
These two trails undergo on 7/25 and 8/26 So we
analysis by it track.
10504 have low activity both open field and hole
board. And also he got a poor balance ability.
10485 10459 express a high number in both exp. We
assume that his curiosity kills the cats.
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Two trails(7.25 8/26) show a big change!! Why??

• I calibrate the field in the different size.
• The wall around us turns white to green.
• The light is different.
• The mice is nerves.
• Above all,I think the first one is the main
  reason
• why I got the difference data.
• But the assume 2 maybe affect the mice feeling,
• we would design a new project to test whether
  its
• true or not.

Improvement
back
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Water maze 3trails/day 10day
Mice are natural swimmers,and they are
intelligent.
Put mice in a circular pool with radius
1.35meter Mice will try to find a place to
stand in the pool This device want to select
which is good or bad in Spatial learning and
memory. Using a new staring point, he swims
direct to the platform not try backtrack to old.
(mouse will think out different strategy to find
the plat)
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Mouse learning behavior in water maze
As mice,what would you do in the pool?

• find a place to stand.
• Path decide by what he used to do
• Set up a spatial map in his brain
• Knowing that platform would always be there
• Use all kinds of strategy to find it.
• Find three remarkable cues on the wall,
• and triangulate to find the platform.
• (spatial learning and memory )

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Data analysis
N
4
1
E
W
2
3
SE
SW
S
Total 26 trails
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What strategy they take?
1.swim around at some distance form the
pool 2.one way to destination 3.backtrack to
previous starting location 4.guess the
direction,and make a series of sweeps 5.looking
at the cues,and triangulate platform
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Two value
1.no find the platform Q(quad4sc
quad4path)0.5 (quad4sc-quad4path)
selection for the worst spatial learning and
memory 2. Find the platform S(1-latent
sc/average)100(1-path mt/average)100
selection for the smart,but not necessary for
spatial .
Video track path is the most reliable method
Remember that What you design,what you get.
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Probe test
There is no platform!! After 8 days train,we test
the mices spatial learning.

• 1. direct to the previous platform
• 2. looking forward something is here
• 3. swim to the center and to quadrant4
  repeatedly
• Stay here
• Ensure that the platform is not here. Swim out.

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selection
Probe test is a basic mean to analyze spatial
learning.
10523 10462 10458 10487
back
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10523
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10462
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10458
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10487
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selection
Probe test is a basic mean to analyze spatial
learning.
back
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Result as follows

• 10504 10505 lack balance ability.
• 10485 good balance ability.
• 10504 is short of activity and curiosity.
• 10485 10459 have high activity and courage

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Next step
Assume that the phenotype is recessive ? aa Use
wild type AA x aa F2 got all Aa
F2 inbred Aa X Aa F3 got
AA Aa aa 1 2
1 Then see if 1/4 offspring carry the same
phenotype (aa) If yes ? abundant mice would use
to analyze.
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Thanks
Byebye