MD ANDERSON

1
Carmona June 19, 2008
2
Maureen Neitz
3
Roorda Williams, Nature 1999
4
(No Transcript)
5
3
4
R5
2
5
7
6
1
6
hn
Rh1

R1-6

7
Rh3
Rh5
R7
R8
Rh4
Rh6
8
p
Rh3
Rh5
y
Rh6
Rh4
R7
R8
9
UV
UV
rh4
rh3
R7
rh6
rh5
R8
Green
Blue
10
otd
spineless
11
spineless
Bob Johnston
12
spineless is necessary for the rh4/rh6 fate
wt
Rh5 Rh6
Rh3 Rh4
R7
R8
Mathias Wernet
13
UV
UV
rh4
rh3
R7
rh6
rh5
R8
Green
Blue
14
(No Transcript)
15
(No Transcript)
16
Thomas Labhart
17
homothorax
Mathias Wernet
18
(No Transcript)
19
Rh4
Rh34
Rh3
20
dorsal y
DRA
Rh3
Rh3
p
Rh3
Rh5
y
Rh3
Rh4
Rh6
Rh6
Rh4
R7
R8
R7
R8
21
Iro-C
R7
Rh3
Rh4
Iro-C
22
R7
R7
Rh3/ Rh5
Rh3/ Rh3
otd
hth
R8
R8
R8
R7
Iro-C
spineless
Rh4 Rh6
Rh3Rh4 Rh6
R8
23
ss
R7
R8
24
What is the signal between R7 and R8?
ss
R7
Rh4
Rh3
X
?
R8
Rh5
25
ss
R7
Rh4
Rh3
R8
Rh5
All Rh5 Lats/warts
26
warts
Rh5 Rh6
wt
melted
Tamara Dvali
27
GMRgtwarts
Rh5 Rh6
wt
GMRgtmelt
28
Rh6, warts
Tamara Dvali
29
melted -/-
warts-/- melted-/-
Rh5 Rh6
Tamara Dvali
30
R7
Wts Lats
Yorkie
R8 Differentiation
31
Are Rhodopsins involved in the fate of
photoreceptors?
Rh5 Rh6
32
rh61 mutant co-expression of Rh5 Rh6-GFP
Rh5 Rh6gtGFP
33
Rescue of rh6 mutants by rh6gtRh6
Rh5 rh6gtRh6 GFP
34
dpp
Melted
Warts
Rh5
Rh6
35
ss
X
ss
X
rh4
rh3
Rh3
signal
Rh4
Dpp
Babo
warts
melted
melted
rh6
rh5
rh6
rh5
36
The larval eye (Bolwigs organ) A simplified eye
37
Larval eye expresses R8 Rh in the same proportion
8 Rh6 (green) 4 Rh5 (blue)
38
Specification of BO photoreceptors is similar to
R8.
Atonal (R8)
39
EGFR recruits Rh6 photoreceptors
wt
EGFRDN
4 Rh5 (blue) 8 Rh6 (green)
4 Rh5 (blue)
40
But also quite different
8 Rh6 0 Rh5
0 Rh6 12 Rh5
8 Rh6 4 Rh5
Spalt Empty Rh5
Svp Rh6 now express Rh5
wt
41
(No Transcript)
42
The eyelet, a remnant of BO
0 Rh5 4 Rh6
43
?
44
Rh6-cells undergo apoptosis
Rh5 Rh6
45
Rh5-cells switch to Rh6
46
EcR is required for Rh6 death
wt
wt
rh6gtEcRDN
47
EcR is required for switch from Rh5 to Rh6
wt
wt
rh5gtEcRDN
48
sens
49
Franck Pichaud
50
Cajal Sanchez, 1915
Fischbach Dittrich, 1989
51
Javier Morante
52
R8
R7
53
R8
R7
54
Javier Morante
55
Logic of the color vision circuit
R8
R7
Interneurons
R7 vs. R8
Pale vs. yellow
Projection neurons
56
Comparing R7 vs. R8
R8
R7
R8
R7
Javier Morante
57
686a-Gal4
Arzu Celik, Tetsuya Tabata Javier Morante
58
Comparing Blue vs. Green
R8
R7
R8
R8
R7
R7
59
2135a-Gal4
Arzu Celik, Tetsuya Tabata Javier Morante
60
High level processing neuron
R8
R7
R8
R7
61
Multi-columnar local neurons
R8
R7
R8
R7
R8
R7
62
1973a-Gal4
Dm
Arzu Celik, Tetsuya Tabata Javier Morante
63
Modulating long-projecting neurons
R8
R7
R8
R7
64
ap
ey
other
dll
Mi1
Mi4
Mi5
Mi1-5
C
Mi8
Mi9
Dm6 (782c-Gal4)
Mi12
Dm3
Mi3
Dm1-3
Local
Dm2
Mi11
Dm4-5
Dm1-5
Pm1 (2402a-Gal4)
NC
Dm4
Mt8
PmLM7
Mt3 (192a-Gal4)
Dm5
Dm1
Pm9-10
Dm8
Pm2
Tm1
C3
Tm10
T1 (c699-Gal4)
Tm2 (otd-Gal4)
TmY5a
R7/8
Tm9
TmY5
Tm3
C
Tm5
Tm4a
R7 R8
Tm5Y
Tm3-6
Projection
T2
TmY1
Tm5
Lawf (1118-Gal4)
Tm4
TmY2
Tm8
Tm5
TmY4
Tm11
Tm7
TmY8
Tm17
Tm12 (2135a-Gal4)
Tm20
NC
TmY9
Tm16
Tm26
Tm25 (2135a-Gal4)
TmPY R7
TmLM7
TmY11
Tm28
TmLM7-OT
TmY13
TmLM8
Tm3-6
T3
Y3
TmY3-6
other
Y1
Tm24
65
(No Transcript)
66
(No Transcript)
67
(No Transcript)
68
(No Transcript)
69
Optomotor response relies on contrast
1.2
1
0.8
0.6
0.4
0.2
0
0
50
100
150
200
250
-0.2
Green 175
(n23)
Blue intensity
Satoko Yamaguchi Reinhard Wolf
70
Rudy Behnia Alberto DelValle Bob Johnston Dave
Jukam Preet Lidder Javier Morante Daniela
Pistillo Simon Sprecher Daniel Vasiliauskas Nina
Vogt Satoko Yamaguchi Arzu Celik, Tiffany
Cook Esteban Mazzoni, Tamara Dvali, Mathias
Wernet Collaborations Mike OConnor, Jessica
Treisman, Reinhard Wolf
71
I was gratified to be able to answer promptly.
I said I dont know.

• Mark Twain

72
(No Transcript)
73
Satoko Yamaguchi Reinhard Wolf
74
(No Transcript)
75
Spineless
76
Appearance of UV, Blue, Green and Red pigments
Red
Green
Blue
UV
77
Neural superposition
RETINA
n
LAMINA
h
A
B
C
D
E
H
B
C
F
G
A
D
E
4
I
J
F
G
H
1
6
5
2
3
J
3
4
I
4
5
2
L
M
5
K
2
6
1
6
K
L
M
1
1
6
N
O
N
O
2
5
Q
P
R
4
Q
S
T
P
R
3
S
T
Adapted from The physiology of insects,
Rocktstein M. et al. (1974)
78
(No Transcript)