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Ozone Testing on Honey Bee Equipment

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Title: Ozone Testing on Honey Bee Equipment


1
Ozone Testing on Honey Bee Equipment
  • By
  • Elmer James
  • Martin James
  • Karla James Shelton
  • Of
  • Slide Ridge Honey
  • Mendon, Utah
  • June 9, 2008

2
Assisted by
  • O3 Company, Aberdeen, Idaho
  • USDA-ARS Bee Research Lab, Beltsville
  • Utah Dept. of Agriculture, Salt Lake City
  • USDA-ARS Pollinating Insect Research Unit, Logan,
    Utah
  • Ozone Research Equipment, Akron, Ohio

3
Lab Facilities
  • Small Test Chamber at Ozone Research Equipment in
    Akron, Ohio
  • Lab Testing at USDA-ARS Beltsville Bee Lab in
    Beltsville, Maryland.
  • Lab Testing at Utah Dept of Agriculture in Salt
    Lake City, Utah
  • Lab Assistance from USDA-ARS Bee Lab in Logan,
    Utah.

4
Full Scale Field Testing
  • Ozone room constructed at Slide Ridge Honey in
    Mendon, Utah
  • Size 20 ft. X 15 ft. X 10 ft.
  • Large ozone generator unit 2050-088, double
    chamber type manufactured by O3 company in
    Aberdeen, Idaho.
  • Monitor with range 0 to 2000 PPM provided by O3
    company.

5
Ozone Room
6
Ozone Room Sealed
7
Ozone Generator
8
Ozone Generator Inside
9
Ozone Monitor
10
Ozone Test Start up Number 1
11
Ozone Test Start up Number 2
12
Ozone Test Start up Number 3
13
Ozone Test Start up Number 4
14
Ozone Test Run Number 2
15
Ozone Test Run Number 3
16
Ozone Test Run Number 4
17
Part of Ozone Test Run Number 4 (USU Data Logger)
18
Ozone Test Run Number 5
19
Ozone Test Run Number 5 (USU Data Logger Record)
20
Observations
  • Pesticides residue destroyed.
  • Herbicides residue destroyed.
  • Wax moth destroyed, inactive remains.
  • Gasoline residue destroyed on outer surface
    during run 5 on a customers Hives.
  • Comb readily accepted by Honey Bees.
  • Dry Rot fungus may be stopped.

21
Observations Cont.
  • Foundation purchased several years ago was never
    acceptable to our Honey Bees. Now that this
    foundation has been submitted to Ozone they seem
    to readily move on to it and start building comb.

22
Items not Subdued
  • FOULBROOD
  • More testing required with different techniques.

23
State of Utah Lab Tests
  • 2008-8 Control Sample of Beeswax Pollen with no
    Pesticides applied after Ozone treatment.
  • 2008-9 Beeswax Pollen with Fluvalinate applied
    and then treated with Ozone.
  • 2008-10 Beeswax Pollen with Amitraz applied and
    then treated with Ozone.

24
State of Utah Sample 2008-8
25
State of Utah Sample 2008-9
26
State of Utah Sample 2008-10
27
Wax Moth Damage
  • Comb with severe wax moth damage has never been
    acceptable to our Honey Bees, therefore these
    frames were disposed of in our operation.
  • Same severe damaged comb, Ozone treated, is very
    acceptable, the bees move right on to it and
    starting cleaning out the cells for reuse. Refuse
    dumped on bottom board by the bees, tip hive and
    scrap out.

28
Typical Wax Moth Damage
29
Typical Damaged Frame After Ozone Treatment
30
Drying out Equipment
  • The Ozone process seems to dry out all of the
    equipment, boxes, frames, comb, pallets, etc.
  • O3 CO theorizes that dry air input as the
    carrier for the Ozone gas is absorbing the
    moisture from the equipment.

31
Room Pressure Exhaust With Charcoal Filter
32
Air Circulation In Ozone Room, NO Rubber or
Urethane Belts
33
Air Circulation in Ozone Room Requires Direct
Drive Blower
34
Coding of Equipment Processed Through Ozone
35
Coded Hive Box Frames
36
Restack Hive Boxes to Allow Better Air Circulation
37
Locate Ozone Sensor Near Top of Equipment Being
Processed
38
Depressurize ducting, route outside of building
39
Cost
  • Ozone generator 3,000.00 per month lease.
  • Electric power 194.94.
  • Frames processed 8,880.
  • Labor 0.00.
  • Cost per brood frame 0.36 each.
  • Not included, improvement to boxes, pallets,
    lids, etc.

40
End Result of Ozone Treated Equipment. (30 Day
Nuc)
41
Honey Bee Care
  • Bees shown in slides have been treated for Mites,
    Nosema, Foulbrood, and Chalkbrood.
  • Their diet has been supplemented with Bee-Pro
    other additives.

42
Conclusions by Slide Ridge Honey
  • All Dead Out Hives should be processed in an
    Ozone Chamber before attempting to raise Honey
    Bees in them.
  • We consider placing Honey Bees in unprocessed
    Dead Out Hives, a waste of time and money.

43
Conclusions Continued
  • Why does Ozone Treatment Work?
  • This was the first full scale Ozone Test under
    field conditions, and we know a number of items
    that are being destroyed, however we may have
    wiped out other detrimental elements that need to
    be known and requires further Lab Research.

44
Comments
  • We wish to acknowledge the USU Bee Lab and their
    staff for coming up with the idea of using Ozone
    to purify Bee Hives, and now that we have proven
    that the idea is economically feasible by Bee
    Keepers at the commercial level, we encourage USU
    Bee Lab to enlarge this research program at the
    earliest opportunity.

45
Comments Continued
  • We believe that with Ozone Processing and proper
    nutrition, CCD may disappear from the Bee Keeping
    operation.
  • We believe that our two biggest problems in bee
    keeping are pesticides/herbicides being returned
    to the hive poor honey bee nutrition.

46
Factor
  • We need for the Lab to establish a factor that
    relates
  • Time in Ozone chamber to PPM of Ozone
  • This would allow us to cut cost on power
    consumption and/or equipment size.
  • We are also of the opinion that the Logan Bee Lab
    is in the best position to conduct this Research.

47
Distribution
  • USDA-ARS Bee Lab
  • 5310 Old Main Hill
  • Room 253
  • Logan, Utah 84322-5310
  • Dr. Rosalind James
  • Craig Huntzinger
  • Ellen Klinger
  • State of Utah
  • Dept. of Agriculture Food
  • 350 N. Redwood Road
  • Salt Lake City, Utah 84114-6500
  • Danielle Downey
  • Dr. David Clark

48
Distribution Cont.
  • Beltsville Bee Lab
  • Room 211
  • 10300 Baltimore AV.
  • Building 476 BARC-East
  • Beltsville, MD 20705
  • Bart Smith
  • Jeffery Pettis
  • O3 Manufacturing
  • 2775 West 1800 South
  • Aberdeen, Idaho 83210
  • Lynn Johnson
  • 208-397-3033
  • 208-220-1288 cell

49
Distribution Cont.
  • Ozone Research Equipment Company
  • 221 Beaver Street
  • Akron, Ohio 44304
  • Ron Brainard
  • 800-742-8535
  • Bee Keepers
  • Newswander Brothers Honey
  • Pendell Apiaries
  • Homan McMaster Apiaries
  • Mike Brockman

50
Distribution Cont.
  • Suppliers
  • Mann Lake Ltd.
  • Stuart Volby
  • 501 1st. St. South
  • Hackensack, MN
  • 800-880-7694
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