Title: My Favorite Pathway: Glycolysis/Gluconeogenesis
1My Favorite PathwayGlycolysis/Gluconeogenesis
- Pallavi Penumetcha
- 11/25/08
2Glycolysis (RAST)
3Glycolysis Pathway Condensed
4Example of How to Investigate One Enzyme
Enzyme 3.1.3.11 was not highlighted in the SEED
pathway
CDD Results
5Pfam Results
NCBI Results
6Enzymes not called by RAST annotation
EC Number and Name Other Databases? Notes
3.1.3.11 (fructose bisphosphatase) Yes SEED Confirmed as fructose bisphosphotase in CDD, Pfam and NCBI
2.7.1.11 (6-phosphofructokinase) Yes SEED and Manatee 5 calls between 2 databases (2 in SEED and 3 in Manatee) 2 sequences in SEED match with two in Manatee?call different EC numbers -SEED 2.7.1.11 -Manatee 2.7.1.56 (1-phosphofructokinase) 1 sequence different in Manatee 2 sequence match same results in CDD Sequence in Manatee has the superfamily related to phosphofructokinases
1.2.1.12 (glyceraldehyde-3-phosphate dehydrogenase, phosphorylating) Yes All three All three have same protein sequence SEED calls the EC number 1.2.1.59 (glyceraldehyde-3-phosphate dehydrogenase NADP, phosphorylating) Shows that the different databases have different criteria for calling a protein
7Fructose and Mannose Metabolism
8Pentose Phosphate Pathway