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A snurp contains a small, nuclear, U-rich RNA (snRNAs ... 1. Primed transcription by SL. 2. Trans-splicing model. Fig. 16.11. Trans-splicing in Trypanosomes ...
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SR proteins have domains rich in serine and arginine. In yeast: the branch-point bridging protein (BBP) binds to the U1 snurp at the 5' ...
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Trixie helped her daddy put the laundry into the machine. ... Snurp. 'Now, please don't get fussy,' said her daddy. Well, she had no choice... Trixie bawled. ...
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It controls a cascade of alternative RNA splicing decisions that finally result in female flies. WHY is splicing a feature of eucaryotes and not procaryotes?
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esone1 introne1 esone2 introne2 esone3 GT GT AG AG SPLICING eliminazione introni introne1 introne2 esone2 esone3 esone1 unione esoni esone1 esone2 esone3 Alcuni modo ...
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Title: PowerPoint Presentation Last modified by: Created Date: 1/1/1601 12:00:00 AM Document presentation format: Other titles
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Adenovirus life cycle. Figure 9.19 Control of replication ... Figure 10.12 Adenovirus. Figure 10.13 Cotranscriptional editing: Paramyxoviruses ...
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Variazioni epigenetiche Modifiche ereditabili, che pu riguardare un cromosoma o l attivit di un gene, che non varia la sequenza nucleotidica.
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* Figure: 13-04 Title: A Mixed Copolymer Experiment Caption: Results and interpretation of a mixed copolymer experiment where a ratio of 1A:5C is used (1/6A:5/6C).
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'RNA silencing pathways in plants', by Alan Herr, Sainsbury Laboratory, John ... Box 10.5 Editing regulates Ebola cytotoxity. Figure 10.15Export of HIV mRNAs ...
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... DNAs of a few highly expressed nuclear genes (e.g., hemoglobin, ovalbumin) ... Loops form from RNA annealing to the template strand and displacing coding ...
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IX: DNA Function: Protein Synthesis
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... second histone (H1 in the illustration) fastens the DNA to the nucleosome ... DNA polymerase on the leading strand can operate in a continuous fashion, RNA ...
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Four stages: Initiation, Elongation, Termination, Post-transcriptional modification ... Dissociation is somehow coupled to ATP hydrolysis. Comparison with Eukaryotes ...
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Base pairing of U2 snRNA with the branch point is thought to displace and activate the adenosine whose 2 OH attacks the 5 splice site * * snRNP snRNA length ...
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Domain I contains binding sites for the 5' exon (keeps the 5' exon from floating ... The Spliceosome Cycle of Assembly, Rxn, and Disassembly. Fig. 14.27 ...
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4) Photocopy (Transcriptional) Regulation. 5) Packaging (Post-Translational) Regulation ... At fertilization, both X chromosomes are active. ...
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'De novo DNA methylation in mammals: As easy as A, B, L' 26.1 DNA ... Actinomycin D inhibition of prokaryotic and eukaryotic RNA Polymerases. 26.2 RNA processing ...
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Frederick Griffith (1928) Conclusion: living R bacteria transformed into deadly S bacteria by unknown, heritable substance Oswald Avery, et al. (1944)
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Title: Transcription Author: Korakod Last modified by: Suranaree Created Date: 9/29/2000 5:35:22 AM Document presentation format: On-screen Show Other titles
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Describe the general mechanism of the spliceosome doing splicing of ... Describe how alternative splicing produces diversity of mRNA products; some RNA self ...
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Genes are viewed as 'ORFS' Open Reading Frames These are ... of extant humans and are therefore observed in all individuals in the human population. ...
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Covers : RNA Processing Translation Genetic Engineering Membrane Transport
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Title: Slide sem t tulo Author: SETOR DE VIROLOGIA Last modified by: cliente Created Date: 1/18/2000 6:13:04 PM Document presentation format: Apresenta o na tela ...
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RNA Metabolism DNA-dependent synthesis of RNA RNA processing RNA-dependent synthesis of RNA & DNA RNA (Ribonucleic Acid) Transcription: an enzyme system converts the ...
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Ch. 17 Lecture Flow of genetic information in a cell How do we move information from DNA to proteins? transcription translation RNA protein DNA trait
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How cells read the genome: from DNA to protein
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Mecanismos generales de mantenimiento de la tolerancia a lo propio y autoinmunidad Dra Marisa Jorf n EDUCACI N CL NICA PERMANENTE SERVICIO DE CL NICA M DICA
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Chapter 17~ From Gene to Protein
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GCGCGC CCAAT TATA BOX 3' TAC EXON! AT INTRON CG EXON ATT5' -100bp -40bp -30bp ... Sees the TATA. Binds to the promoter( conserved or similar in eukaryotes) ...
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4. Enhancers and Silencers ... Enhancer & Silencer. 12. 5. Upstream ... Enhancer ??? RNA pol II: Stimulate transcription ??? promoter????? consensus sequences ...
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Chapter 17~ From Gene to Protein * * * * * * Strong evidence for a single origin in evolutionary theory. * * * * The tRNA-amino acid bond is unstable.
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Title: Diapositiva 1 Last modified by: Usuario Created Date: 1/1/1601 12:00:00 AM Document presentation format: Personalizado Other titles: Arial Times New Roman ...
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gene expression from DNA to protein biology 1 Genes control metabolism Gene expression is a two stage process transcription translation Genes consists of triplets ...
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* * * * Meselson-Stahl Experiment * * * * * * * * Replication in E.coli * Replication in Eukaryotes * Comparison * Enzymes involved Single strand binding protein ...
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Evolu n genomika Eduard Kejnovsk Kapitola II.: Relikty sv ta RNA
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PLAN I. Support et organisation de l'IG II. M canismes mol culaires de conservation de l'IG III. M canismes mol culaires de l'expression de l'IG
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Poly (A) Tail 30 to 200 adenine nucleotides ... Nucleus only. Ribonucleic Acid. Single stranded. A, G, C, U. Nucleus and cytoplasm. Fin ...
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Protein structure NOT covered- but be familiar with the terms- primary, ... Svedberg unit. rRNA's: are mostly on one transcript that's processed, not spliced. ...
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Chapter 17 From Gene to Protein Insertions & Deletions The addition or loss of a base in the DNA. Cause frame shifts and extensive missense, nonsense or sense mutations.
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only transcribes tRNA genes. each has a specific promoter sequence it ... Alternative mRNAs produced from same gene. when is an intron not an intron...
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Core and holoenzyme are all thought to be DNA bound. VERY little is free ... an excess of nonspecific sites to adsorb proteins in crude lysates that will ...
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actinomycin D: prok/euk. rifampicin: prok. a-amanitin: euk. pol II etc. G. C. A Complex of Actinomycin D and DNA. RNA Metabolism. DNA-dependent synthesis of RNA ...
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(PRICE LOWERED)Biochemistry for Medical Students
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... sequences known as 'splicing enhancers', however, additional factors which are ... compensating weak 3'-sites by binding exonic splicing enhancer sequences ...
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Chap. 8 Post-transcriptional Gene Control Topics Processing of Eukaryotic Pre-mRNA Regulation of Pre-mRNA Processing Cytoplasmic Mechanisms of Post-transcriptional ...
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SPLICING. Lecture #4. Eukaryotic genes are mosaics of Int (non coding) and Exons (coding) Exons typically small (150 bp average) Introns: can be small or huge and MANY ...
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... activator protein cAMP required for good Pol binding to promotor (=enhancer) ... Enhancers, Silencers & Response Elements. DNA Binding Motifs ...
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The borders between introns and exons are marked by specific nucleotide ... the message changes codons and reading frames, completely altering the 'meaning' of ...
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Figure 28.31 of Mathews. Secondary structure model of human ... RNA enzyme = Ribozyme' (look at pages 395-397 in Mathews) This type of splicing requires only: ...
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Suggested genes control enzymes that ... George Beadle and Edward Tatum ... Know Beadle and Tatum. Know the central dogma. Be able to 'read' the genetic code. ...
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No direct DNA participation in translation ... Cell-free translation system. In 1961 mRNAs not yet isolated. polynucleotide phosphorylase ...
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What Is the Genetic Material. Thought to be proteins. 20 amino acids can create greater variety. DNA has only different bases. A, T, C, G ...
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... mechanism of action of the transcription inhibitors rifampicin and actinomycin D. ... The intercalated part of actinomycin D is orange. ...
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... RNA polymerase II TFIIF TFIIE TFIIH TFIIH is the only transcription factor with enzymic activity. 2 subunits of TFIIH unwind the DNA C-terminal Domain CTD of RNA ...
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