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SILAC (Stable Isotope Labeling with amino acids in Cell Cultures) C13, N15, O18 t panyaggal vissz k be jel lt aminosav Olyat kell v lasztani, ...
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... motif decomposition of the phosphotyrosine proteome Mass spectrometry-based proteomics Mass spectrometry-based ... Isotope Labeling with Amino ... Peptide ratio ...
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CSE182-L13 Mass Spectrometry Quantitation and other applications
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Random oligonucleotide pool Affinity matrix Yeast One Hybrid Y1-n Bait DNA sequence Library protein TATA Repoter (his, lacZ) Chromatin Immunoprecipitation ...
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Title: Diapositive 1 Author: Liebaert Last modified by: Andras Budiszky Created Date: 10/25/2004 3:03:35 PM Document presentation format: On-screen Show
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Title: PowerPoint Presentation Author: Alain Garnier Last modified by: Alain Garnier Created Date: 1/1/1601 12:00:00 AM Document presentation format
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What is the importance /function of genes. Can we learn all ... Hydrophobicity. Catalytic, structural, regulatory. Complexes. Dynamic. Arabidopsis. Plant Cell ...
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Proteomics Technology and Protein Identification Nathan Edwards Center for Bioinformatics and Computational Biology
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Bioinformatics. New Instruments. Thermo Electron LTQFT. 400K resolution, 0.5-3ppm ... Trained in bioinformatics. Upgrading all systems. GenoLogics Proteus LIMS ...
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Mass spectrometry-based proteomics. Nature ... MS Web addresses of some representative internet resources for protein identification from mass spectrometry ...
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'Proteomics includes not only the identification and quantification of proteins, ... http://sashimi.sourceforge.net/software_glossolalia.html ...
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Gel Filtration Chromatography The method mostly involves the separation of the proteins based on its molecular size. This method is also known as Size exclusion ...
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Affinity chromatography is based on the principle of specific interaction between the protein or antigen and antibody for separation of biomolecules
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High-throughput capability. Relative low costs. Ambiguous results difficult to interpret ... Ions enter the time-of-flight analyzer through a grid and are ...
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Facility for Genomics and Proteomics and UFSNBN Bioinformatics Node
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Methodology for MALD-TOF data analysis Once the peptide mass data of individual protein is obtained from MALDI-TOF, the next step is for identification of protein ...
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... SELEX Yeast One Hybrid Slide 14 Slide 15 Verifying a Putative Interaction Equilibrium constant measures the strength of interaction Range of Biological ...
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... Director, Research Center For Genetic Medicine Children's National Medical Center. The A. James Clark Endowed Chair. The Parsons Family Foundation. US ...
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Driving force of Technology Development ... PNGaseF (remove the sugar moiety) ... in H218O to remove the sugar moieties and to incorporate the 'glycosylation-site ...
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Quantitative proteomics - ICAT a The identification and quantitation of complex protein mixtures have been facilitated by MS-based quantitative proteomic techniques.
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How to make the GSL work for you Platforms, Tools, and Services Jennifer Schaff, Interim Director Genomic Sciences Laboratory ...
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Actinomycin D (complexes with DNA and inhibits cellular DNA-dependent RNA synthesis) ... even in the absence of cellular transcription (Actinomycin D-resistant) ...
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Zoom scan (to determine charge) MS/MS scan (optional peptide sequence) Mass Analyzers ... Zoom scan. m/z (m 1)/z (m 2)/z (m 3)/z. Mass Analyzers. Precursor ...
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Control of the cancer cell phenotype using drug combinations ... class of diseases exhibiting uncontrolled growth, tissue invasion and metastasis. ...
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Toward this end, retroviruses expressing FLAG-tagged versions of Erk1, Grb2, ... Immunoprecipitation with anti-FLAG antibodies showed that the selected cells ...
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Bioinformatik - Ein ideales Fach, um mathematisch-naturwissenschaftliche F cher (Biologie, Chemie, Mathematik) mit der Informatik zu verbinden
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Proteomics Facility, School of Biological and Biomedical Sciences, ... gradient elution and MS analysis. nanoflow HPLC system / mass spectrometer Duty Time ...
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Supplementary material for the last-time lecture. More on sequencing by MS ... (2)Typhoon Variable. Mode Imager (3) DeCyder 2-D. Differential Analysis. Software ...
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A biased look at Biomarkers Summed Ion Intensity (~75,000 Spectra) Reporter Group Placement: Selection of Quiet Region Control Example: Time course labeling ...
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Electrophoresis. Separate based on size and shape. Fluorescence ... 2D Gel-Electrophoresis. Protein separation. Molecular weight (Mw) Isoelectric point (pI) ...
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Alfred Wegener (1880-1930) Continental Drift. Matching Geologic ... Abyssal Plain off New Jersey (4000-6000 m) Deep Ocean Basins. Hot Spots and Island Formation ...
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NEBOSH NATIONAL GENERAL CERTIFICATE Occupational health hazards LEARNING OUTCOMES (I) classification of occupational health hazards (physical, chemical, biological ...
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contaminant directed into a large hood by fan assisted draught. captor hood ... horses, donkeys, mules. CONTROL STRATEGIES FOR ZOONOSES. routes of entry. skin ...
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Paleolithic period ('old stone') (c. 40,000-10,000 BC) ... in the Magdalenian of the Pyrenees some paintings (Niaux, Fontanet) had been ...
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... crust: composed of basalt and gabbro, continuously formed at spreading centers, ... massive gabbros formed in magma chambers. peridotites from the upper mantle ...
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... etc) with XPRESS, ASAPRatio, and Libra; and tools for visualization of and ... Libra performs quantification on MS/MS spectra that have multi-reagent labeled ...
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Upon phosphorylation, the b-, and y-ions shift in a characteristic fashion. ... A simple trick can let us predict the modification sites? Consider the peptide ASTYER. ...
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Upon phosphorylation, the b-, and y-ions shift in a characteristic fashion. ... Can we predict the sites of the modification? A simple trick can let us predict ...
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