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Bamboo Mosaic Virus BaMV and satellite RNA satBaMV

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Satellite RNA variants associated with bamboo mosaic virus (BaMV) exhibit ... BSL6 led to the ability of chimeric satBaMV to interfere with BaMV replication ... – PowerPoint PPT presentation

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Title: Bamboo Mosaic Virus BaMV and satellite RNA satBaMV


1
Bamboo Mosaic Virus (BaMV) and satellite RNA
(satBaMV)
satBaMV
(B)
20K
5
(A)n 3
0.8 kb
P20
(C)
(D)
6.4 kb
2.0 kb
1.0 kb
satBaMV
EtBr
(E)
2
Satellite RNA variants associated with bamboo
mosaic virus (BaMV) exhibit different phenotypes.
SatBaMV variant BSL6 significantly reduces BaMV
replication and suppresses the BaMV-induced
symptoms in Nicotiana benthamiana plants, whereas
BSF4 does not. One hypervariable (HV) region
which forms a conserved apical hairpin stem-loop
(AHSL) structure was found in the 5 untranslated
region (5 UTR) of many natural satBaMV isolates.
Interchanges of AHSL segment of HV regions
between BSF4 and BSL6 led to the ability of
chimeric satBaMV to interfere with BaMV
replication and symptom expression.
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