Construction of GatewayTM-based vectors for high-throughput cloning and (co-)expression screening in Escherichia coli Didier Busso, Loubna Salim, Edouard Troesch ...
All vectors are derived from the pET22b vector (Merck - Novagen) ... An aliquot of eluted fraction (10 l) was loaded onto SDS-PAGE. ORF-His6. GST-ORF-His6 ...
Break the Ice when a group is first starting to work together ... demonstrate how difficult it is to break into a new group, especially when you are different. ...