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TRANSFORMATION- inserting the plasmid into the bacteria. ... If bacteria colonies are produced, they are picked and grown in TB media. ...
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Clones code for a tagged protein (GST, FLAG, HA...). When an anti-tag ... Challenges of Cloning. B.anthracis Genome. AT richness of ORFeome. optimize PCR condition ...
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Sense. Anti-Sense. Detect. Fetch. Six frames. Sequence. 24. ChipViewer: Data fetching ... Output of expression data mining output of the expressing regions ...
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False positives. ??????? ???? ????? ?? ?????. Counting interactions ... ????? ?? ???? ? 50% false positives. ????? ????? ????? ?? ?????. ...
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Plant Genomics and Mathematical modelling: a recipe for Systems Biology Martin Kuiper Computational Biology Division Department of Plant Systems Biology
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Gene expression levels correspond to protein levels ... Precipitate Locally low signal Comet-tails (donut hole) Two Types of glass- microarrays ...
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strategies to analyze molecular interactions in intact cells ... Braun et al., Nature Methods, revised manuscript under review. ArrayMAPPIT screening assay ...
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Genes are characterized using gene annotation tools (Pedant, Magpie, etc.) How Many? ... Complement of all genes in a cell, tissue, organ or organism. Transcriptome ...
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Sites (EST) database of same organism, or cDNA sequences if available ... Fugu. worm. E.coli. Gene finding: splicing. cis-splicing of genes ...
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... Harvard and MIT and Dana-Farber Cancer Institute, 2Center for Cancer ... the Center for Cancer Systems Biology (CCSB) at the Dana-Farber Cancer Institute. ...
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