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19.3 DNA is coiled in arrays of nucleosomes. 19.4 Nucleosomes have a common structure ... Photograph kindly provided by Pierre Chambon. ...
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Regulation by changes in histones, nucleosomes and chromatin Opening and activation Movement from heterochromatin to euchromatin Nucleosomes and transcription factors
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Dynamics of assembly and disassembly of nucleosomes along a stretched DNA Ranjith Padinhateeri work done with Jie Yan and John F. Marko University of Illinois at Chicago/
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The genomic code for nucleosome positioning. DNA double helix ... Sandman & Reeve, Curr. Op. Microbiol. 2006 Nucleosomes. nucleosomes. Acknowledgements ...
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Research Objective. investigate the position of nucleosomes in Arabidopsis thaliana. study the relationshi p of DNA methylation with nucleosome. positions
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Chromatin Remodeling DNA is wrapped around histones to form nucleosomes Chromosome packaging Acetylation =acetyl groups are added to histones Exposes the primer so ...
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... degree of chromatin compaction? Eukaryotic Chromatin Compaction ... First Level= Chromatin organized as repeating units. Nucleosomes. Histone proteins are basic ...
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Chapter 11 - The Control of Gene Expression Eukaryotic Gene Regulation DNA is packed in to the nucleus Histones Nucleosomes Helical fiber Supercoil Chromosome What ...
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Denaturation/Renaturation. Denaturation: separation of the 2 strands of the ... Toroid or. spiral. Example: DNA wraps around. histone protein core within nucleosomes; ...
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1 School of Computer Science Telecommunications and. Information Systems ... 'beads on a string' form of Chromatin. 30 nm chromatin fiber of packed nucleosomes ...
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Seen in the nucleus during interphase ... EM shows chromatin to be linear arrays of spherical particles 'beads on a string' = nucleosomes ...
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for steric hinderance between. nucleosomes (weighted conditional. probabilities) ... nucleosome-nucleosome interactions and steric hinderance constraints ...
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Differing DNA sequences exhibit a 5,000-fold range of affinities for nucleosome formation ... DNA sequence motifs that stabilize nucleosomes and facilitate ...
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Transcriptional silencing is associated with Modification of nucleosomes that alters the accessibility of a gene to the ... Sir2 deacetylates nearby nucleosome.
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A genomic code for nucleosome positioning DNA double helix Nucleosomes Chromosome Felsenfeld & Groudine, Nature (2003) p
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Abbreviations. Bases, nucleosomes, and nucleotides have related names. Abbreviations of the form NMP stand for nucleoside monophosphate; 'd' indicates ...
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transcription machinery must work around' nucleosomes. Eukaryotes have a nucleus ... they exhibit variable sensitivity to -amanitin ( a poison from a toadstool) ...
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ds DNA predominates in vivo ... Model of the 30nm chromatin fiber shown as a solenoid or helix formed by individual nucleosomes ...
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With so much DNA in a cell, how is it organized or packaged? ... A cylinder of tightly coiled nucleosomes 30 - nm in diameter. Looped Domains ...
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Order of events in producing a mature mRNA from a pre-mRNA with 1 intron. ... acetylation (right) causes localized unpacking of nucleosomes, which enhances ...
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Order of events in producing a mature mRNA from a pre-mRNA with 1 intron. ... the nucleosomes condense into a solenoid; this inhibits factor binding to DNA targets. ...
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transcription machinery must work around' nucleosomes. Eukaryotes have a nucleus - no coupled Transcription/Translation ... Silk-worm silk glands: FIBROIN ...
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Require General Txn Factors (or GTFs) Txn in chromatin/nucleosomes complex ... an excess of nonspecific sites to adsorb proteins in crude lysates that will ...
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histones (H2A, H2B, H3, H4) octamer generates nucleosomes ... When pulled-apart, ssDNA will self-anneal or try to re-anneal with complementary strand ...
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CTAACCTGGCTACAGAATCATAAGTTGAATTCGACAGGTTATCAGCAACAACACAGTCATATCCATTCTCAATTAGCTCTACCACAGTGTGTGAACCAATGTATCCAGCACCACCTGTAACCAAAACAATTTTAGAAGTACTTTCACTTTGTAACTGAGCTGTCAT ...
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REPLICATION INITIATION IN EUKARYOTES. Five DNA polymerases are known in mammalian cells: ... is involved in nuclear DNA replication and has not been shown ...
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The Eukaryotic Chromosome: An Organelle for Packaging and Managing DNA
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A biophysical approach to predicting intrinsic and extrinsic nucleosome positioning signals
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Chromatin Structure and Gene Activity. 13.1 Histones. Kinds: H1, ... Extremely abundant: the mass of histones in eukaryotic nuclei is equal ... Xenopus ...
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The Drosophila SWI/SNF ATPase Brahma localizes to active regions of the genome ... 'Imitation switch' isolated first in Drosophila by homology with SWI/SNF ...
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Learning outcomes. To learn the central dogma of molecular biology. To have an understanding of the composition, types and structure of DNA and RNA.
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... To study the assembly and disassembly of chromatin under ATP conditions so as to measure the lengths forces and free energy associated with chromatin.
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Unit 3: Interphase Nucleus
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... for transcription Chromatin remodelers also play a role in recombination and repair Chromatin Remodeling Complexes PARP1 and the DDB1-2-CUL4A complex are ...
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GNAT show seq similarity to Gcn5 HAT. MYST. p300/CBP. GTF HAT's ... Red GNAT HAT domain. Pink MYST homology region; HAT. Light Blue & Green Zn fingers ...
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DNA or RNA in different viruses. Single-stranded or double-stranded in ... an electron microscope, you see 100 angstrom beads on a string (Olins and Olins 1973) ...
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Individual chromosomes can be seen only during mitosis. During interphase, the general mass of chromatin is in the ... the silent cassettes in yeast mating type ...
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Heterochromatin. Euchromatin. Facultative (X-chromosome) Constitutive (Telomere, Centromere) ... Transcription Factors Protect 5S sRNA Genes. from Repression ...
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Chromosomes and gene regulation. DNA is just information - a 'blueprint' ... White eye gene. inversion. Chromosomes in nucleus ...
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Afsha Rais * The interest in histone deacetylase (HDAC) inhibitors began almost 30 years ago during some studies designed to understand why dimethyl sulfoxide (DMSO ...
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Kinds: H1, H2A, H2B, H3 and H4. Extremely abundant: the mass of histones in ... 20% of their amino acids are arginine or lysine , have a pronounced positive ...
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Title: PowerPoint Presentation Author: Gil Ast Last modified by: user Created Date: 7/13/2000 2:30:28 PM Document presentation format: On-screen Show
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A Genomic Code for Nucleosome Positioning ... Bending is facilitated by certain dinucleotides placed at the right positions. ... Nucleosome positioning current views ...
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Central Dogma: DNA to RNA to Protein. ... Promotion of cruciform structures by DNA underwinding. Mechanism of Type I topoisomerase action ...
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Chromosomes and DNA Replication Section 12 2 This section describes how DNA is packaged to form chromosomes. It also tells how the cell duplicates its DNA before ...
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Site exposure via stepwise unwrapping from one end of the nucleosomal DNA ... Site exposure at internal nucleosome target sites: Cy3-labeled DNA constructs ...
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DNA is packaged into the heads in a very compact manner. DNA is not ... http://info.bio.cmu.edu/Courses ... one H1 on the outside of the bead' Chromosomes ...
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1. 10 nm fiber - does not require H1 histone under low ionic conditions ... B. Coiling of series of beads into a ... two classes: the GNAT and MYST families ...
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Chromosomes, Chromatin, and the Nucleosome Chromosomes: DNA associated with proteins The chromosome is a compact form of the DNA that readily fits inside
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Chromatin Modifications
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Most of our models of enhancer activity derive from these kinds of experiments ... of factors (rather than any one factor) determines enhancer function ...
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Epigenetics Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520 * So we went about looking at the data more. For each specific region, we combined all the tags from ...
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DNase I cleavage pattern compared to standard nucleosome ... 1) Performed same experiment as before, but analyzed MNase cleavage pattern at high resolution. ...
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30 nm chromatin fiber or solenoid: created by coiled 11 nm fiber- interphase ... NO gene transcription in solenoid. Gene transcription possible ...
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RNA transmits information from DNA into cellular structure ... String wrapped around a series of styrofoam balls. Histone-Depleted DNA ...
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The gene is in an ' active' state only in the cells in which is expressed ... Butyric acid. Trichostatin A. Chromatin Analyses ...
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