T T C G A. A. G. C. T T. A. 5' 3' 3' 5' Sticky. ends. 5' 3' 3' 5' ... ends to pair, join cut ends. with DNA ligase. Bacterium. Main bacteria DNA. Plasmid. 0.5 m ...
These enzymes require a certain temperature to avoid denaturing and for efficient digestion. ... to test the optimal time and temperature of HindIII digestion. ...
We will run lambda DNA without restriction enzymes as well. 3 vials Lambda DNA Lambda DNA cut with EcoRI Lambda DNA cut with HindIII Lambda Phage DNA Genomic DNA ...
RFLP was developed at the late 70's due to the discovery of ... BamHI (Bacillus amyloliquefaciens); HindIII (Haemophilus influenzae); TaqI (Thermus aquaticus) ...
Juan Carlos Gallego-G mez. PECET Sede de Investigaci n Universitaria SIU-UdeA. ... pHIT/G. pSUPER-PURO. HindIII. BglII. pNL-SIN-CMV-GFP. ClaI. XbaI. Mir30-BACE 1 ...
Two DNA Size Standards. One Kilobase Ladder. A Synthetic Mixture of Molecules (3 kb brightest) ... Lambda DNA cut with the restriction enzyme (RE) HindIII ...
Both O.aries and Mus musculus aldolase 2 - 90% similarities ... special characteristics : - presence of EcoRI and HindIII enzyme cleavage. sites in the MCS ...
... genes -lactamase can destroy penicillin and other -lactam antibiotics Kanamycin interferes with Ribosomes Plasmids can be cut with restriction enzymes ...
RFLP (Restriction Fragment Length Polymorphism) RFLP RFLP was developed at the late 70 s due to the discovery of restriction enzymes (REs; or called as restriction ...
... + ++ ++ ++ ++ ++ ++ ++ plasmid adhesion zone phospholipids calcium ion Transforming Escherichia coli With a Recombinant Plasmid GFP Expression ara C gene gfp ...
Palindromes How do you know if a sequence is a palindrome? ATTA? EcoR1 (rasmol image) From E. Coli Palindromes How do you know if a sequence is a palindrome?
Yeast Artificial Chromosomes (YACs) 200-2000 kb. Bacteriophage ... P1 recombinant viability. loxP. B. S. pac. kan. loxP. B. S. insert too large. maximal insert ...
Single sites for a number of r.e.'s, preferably in genes ... ( 1978), pg 61 in Old & Primrose. Transformation reactions. Input reagents. DNA. plasmid ...
What Is Biotechnology? Any technique that uses living organisms or substances from those organisms to make or modify a product improve plants or animals
Hind III 400. Bam HI 2275. Bgl II 600. KanR. Extract the fragments for the gel ... Digest with Hind III and Bam HI. 3755. 2332. 1875. E. coli strains ...
Making Gene Knock-out Construct Clone mouse M-Gene show mice have M-Gene By southern blot analysis Mouse genomic DNA cut with REs Human M-Gene cDNA as probe Pattern ...
WHAT ARE RESTRICTION ENZYMES? Restriction Enyzmes molecular scissors able to cut DNA HOW DO RESTRICTION ENZYMES WORK? Usually cut DNA at a palindrome such ...
Overhangs re-anneal. Removal of 5' Phosphate. Prevents re-ligation of vector ... cloning or using a single restriction site on the vector where it may re-anneal ...
PCR Scelta dello stampo Scelta dei primer Vettori a singolo filamento Fagemidi Strategie di sequenziamento Primer specifici consecutivi Delezioni progressive ...
Sticky ends are only compatible with an. end that leaves the complementary single ... Single stranded overhang aids ligation. preligation complex lasts longer. ...
... (RFLP) Agarose gel electrophoresis Molecular weight determination Simulation of DNA Fingerprinting Plasmid mapping DNA Fingerprinting Real World ...
Recombinant DNA (DNA Cloning) SC.912.L.16.12 What is DNA cloning? When DNA is extracted from an organism, all its genes are obtained In gene (DNA) cloning a ...
Figure S1. Construction of pAL70. The Hyg cassette was amplified from pIJ963 (Lydiate et al. 1989) using Pfu polymerase (Stratagene) and oligonucleotides RP594 and ...
Understand the use of restriction enzymes as biotechnology tools. Become familiar with principals and ... Electrolysis: the splitting of water using electricity ...
Restriction Enzyme Cleavage of DNA Carolina Kit Timeline Week Before: HW DNA scissors, DNA goes to the races Monday pour gels Tuesday Lecture, Run gels with DNA ...
AP Biology: Lab 6 In the early 1970s scientists discovered the genetic code is universal - the same for all living things. This has enabled scientists to combine DNA ...
A typical example of alcA-regulated gene expression. in an Aspergillus nimO18 mutant ... When the alcA::rif1OE strains are grown on inducing carbon sources ...
Restriction Enzymes. Molecular Scissors. Restriction enzymes are molecular scissors. Restriction Enzymes scan the DNA code. Find a very specific set of nucleotides ...
Characterization of a two-component immobilization system through GST ... Covalent bonding. Site specific immobilization using antibodies. Immobilization System ...
Son endonucleasas espec ficas capaces de reconocer secuencias palindr micas de ... que se salgan de la fosa, y bromofenol azul que ti e la muestra de color azul y ...
Just Like Scissors Cut Paper. How Restriction Enzymes Cut. Some RE cut the foreign DNA ... Molecular Scissors for Specifically Cutting DNA.' Molecular Biology. ...
Digest Final PCR product with Hind III and Kpn I; isolate 474 bp fragment ... EtOH precipitated, gel-pure vectors (digested with Hind III and Kpn I) DCLD. DB 1 2 ...
100's of DNA markers mapped onto each chromosome. high density linkage map. ... Mapped gene to end of chromosome 4 using RFLPs and other polymorphic. DNA ...
To understand the application of recombinant DNA technology. To understand the functions of the recombinant protein. To learn how to design a relevant cloning strategy.
DNA is the Flash but Proteins are the Cash of Biotech pAmylase Restriction Digestion (Lab 8b) ~40 bp Ellyn Daugherty SMBCP, San Mateo CA www.BiotechEd.com