Gyrase PowerPoint PPT Presentations

Title: Sulfonamides,Trimethoprim and Quinolones Last modified by: Sushike Created Date: 5/26/2003 11:39:43 PM Document presentation format ...

Antifolates, DNA gyrase inhibitors, Novel lipopeptides Pawitra Pulbutr M.Sc. In Pharm (Pharmacology) ...

Quinolones Drugs: norfloxacin, ciprofloxacin, ofloxacin, levofloxacin, moxifloxacin Mechanism of action: Inhibit bacterial DNA synthesis by inhibiting DNA gyrase and ...

MECHANISM In gram- bacteria, DNA gyrase is the primary target. In gram + bacteria, topoisomerase IV is the primary target (newer quinolones). PHARMACOKINETICS Rapidly ...

4 3 2 1 1 = initiator proteins 2 = single strand binding proteins 3 = helicase 4 = topoisomerase (gyrase) Review: Proteins and their function in the early stages of ...

DNA REPLICATION DNA gyrase: the bacterial enzyme that relieves the tension produced by the unwinding of DNA DNA helicase: enzyme that unwinds double-helical DNA by ...

... skin infections, bones, everything Mechanism of action Inhibition of bacterial DNA gyrase Adverse effects GI reactions, dizziness, headache, fatigue, ...

Inhibit bacterial gyrase ... bacterial diarrhoea caused by shigella, salmonella and E. coli Mechanisms of bacterial resistance to quinolones: ...

Kornberg's enzyme: DNA polymerase I or 'Pol I' ... Helicase: Unwinds double helix. DNA Gyrase ('topoisomerase'): Relieves unwinding tension. ...

Antibiotics Inhibition of DNA synthesis Resistance to Quinolones Is due primarily to chromosomal mutations that modify the bacterial DNA gyrase.

Relieves torsional strain generated by DNA unwinding. DNA gyrase (DNA topoisomerase II) ... the leading strand proceeds as DNA is unwound by the DnaB helicase. ...

... AZ/NYMC Whole-Cell Hit to Lead Program AZ Mycobacterial Gyrase Inhibitors GSK InhA Inhibitors GSK TMC207 Tibotec Moxifloxacin (+ R, Z, E) ...

DNA helicase: unwinds the double helix by breaking the H-bonds ... gyrase: relieves tension from the unwinding of the DNA strands during bacterial replication. ...

DNA unwinding. Protein recruitment. Priming and DNA synthesis. The functions of initiator ... unwinding. DNA gyrase (DNA topoisomerase) Binds single-strand DNA ...

DNA gyrases (unwinding the molecule), and. DNA ligases (concatenating segments together) ... By unwinding the DNA double helix separating the 2 strands. ...

Local unwinding during replication results in overwinding or supercoiling of ... Wasserman & Cozzarelli, Science 1986. Topoisomerases. Type I topoisomerases: ...

in vivo Dr. Sevim I k Type II mechanism Reactions catalysed by topoisomerases Prokaryotes: Reactions catalysed by topoisomerases Eukaryotes: Functions of ...

Title: PowerPoint Author: VAIO Last modified by: pharm Created Date: 10/3/2003 1:43:49 AM Document presentation format:

Synthetic compounds consisted of an N1 alkylated 1,4-dihydro-4-oxo-3 ... action of nalidixic acid and its congener is the result of the inhibition of DNA synthesis. ...

Aminoglycosides Intro Group of antibiotics used in the treatment of bacteria infections aerobic G-ve Consists of 2 or more amino sugars and a hexose nucleus Serious ...

Effective against Gm ? rods, Gm , anaerobes; effective against most -lactamases ... Disease caused by Bacillus anthracis, a gram-positive spore-forming bacteria ...

PROPERTIES OF DNA II. Supercoiling and Topoisomerases The E. coli chromosome 2 puzzles 1. It was circular and twisted 2. Chromatograph showed evenly spaced bands ...

1 To every thing there is a season, and a time to every purpose under the heaven:

Structural approach towards endotoxin and ... Laboratory of Biotechnology, National Institute of Chemistry (NIC), Ljubljana, Slovenia ... Branka Wraber-Herzog ...

Desiccation. Radiation: x-rays or gamma rays. UV. CHEMICAL CONTROL ... Antibiotic resistance - Kirby-Bauer or sensitivity disc assay. Antiviral drug targets ...

MECHANISMS OF ACTION OF ANTIBIOTICS BACTERIOSTATIC AGENTS Sulfonamides Drugs inhibiting protein synthesis except aminoglycosides (macrolides, chloramphenicol ...

Bond vibrations 1 fs (10-15 s) Sugar repuckering 1 ps (10-12 s) DNA bending 1 ... DNA: global and local 3'3' stretching. TBP induced deformation. X-ray Model ...

College of Medicine, The Catholic University of Korea. Prevention of endophthalmitis ... After removal of aqueous humor 0.1ml using 30 gauge needle ...

Geomicrobiology - University of Vermont ... Cell structure

Inhibitors of Protein Synthesis

DNA REPLICATION AND REPAIR ... Hydrogen bonds break between bases Each strand acts as a template Resulting in 2 identical DNA molecules Semiconservative ...

Title: DNA replication Subject: genetics Author: Mark Keffer Last modified by: Mark Keffer Created Date: 1/10/2001 1:28:07 PM Document presentation format

DNA Replication-III * * The origin of replication in E. coli is termed oriC origin of Chromosomal replication Important DNA sequences in oriC AT-rich region DnaA ...

Then, an insulin receptor-like gene, daf-2, was reported in the nematode Caenorhabditis elegans. ... ancestor of mammals and nematodes, more than 600 million ...

Title: PowerPoint Presentation Last modified by: biochem2 Created Date: 1/1/1601 12:00:00 AM Document presentation format:

Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...

Topological Problems in Replication Linear Chromosomes: Telomerase for replication of the ends Topoisomerases to relieve strain of untwisting and supercoiling

Title: Aucun titre de diapositive Author: grpa4375 Last modified by: user1 Created Date: 4/26/2004 3:04:28 PM Document presentation format: Affichage l' cran

Helicases unwind the parental double helix. ... Primase synthesizes a short RNA primer, which is extended by DNA polymerase to ...

DNA Replication in Prokaryotes and Eukaryotes. Overall mechanism ... Is DNA replication bidirectional? Does DNA replication start at the same location or ...

Chloramphenicol. Binds 23S rRNA on 50S subunit. Inhibits peptidyl transferase activity ... blood dyscrasia (chloramphenicol) hemolytic anemia (sulfonamides) ...

Prior to 1977 they were considered bacteria ... Facultative and Obligate Anaerobes and Aerobes. How do Archaea tolerate the heat? ...

MarA and SoxS (E.coli) Transcriptional regulation. Direct or indirect ... MarA and SoxS can both be overexpressed in a quinolone-resistant strain. CONCLUSIONS ...

To develop a new class of. broad spectrum antibiotic. based on bacterial. quorum sensing. ... Antibiotic Cellular Targets. Density regulated gene products. Y ...

Replication = 'duplication of DNA. giving rise to a new ... Stahl. isotopes. Replication. is bidirectional. Replication. forks. Origin. One strand is formed ...

Pol III holoenzyme of E. coli can synthesize hundreds of ... Also most bacteria that cause enteritis: Salmonella, Shigella, E. coli. ...

Effets adverses. Contre-indications. Effets adverses. G n ralit s ... Effets adverses conna tre. R sistances : savoir utiliser bon escient ...

Stabilizes topoisomerase I-DNA intermediate, preventing DNA strand re-ligation ... There are enzymes that will cut DNA, ligate DNA, and change the topology of DNA. ...

MIC zone of inhibition. Affects include amount of inoculum, concentration of agent, ... Resistance predates the use of the drugs. Why natural resistance exists ...

Title: Author: tengzan Last modified by: Administrator Created Date: 4/24/2003 3:25:34 AM Document presentation format

DNA structure/topology. Genomes are longer than the cells in which they are packaged ... Review of Watson/Crick DNA Structure. major groove. minor groove. 34 ...

GENETICS

Severe Gram negative bacterial infections due to susceptible ... Bacterial gastroenteritis. LRTI. Sinusitis. Skin and soft tissue. Bone and joint. Bacteremia ...

Title: PowerPoint Author: win Last modified by: user_mother Created Date: 9/24/2001 9:26:49 AM Document presentation format:

Sterilization (or sterilisation) refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents (such as fungi, bacteria, viruses, spore forms, prions, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media. Sterilization can be achieved through various means, including: heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization, in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents which are present.

... degree of chromatin compaction? Eukaryotic Chromatin Compaction ... First Level= Chromatin organized as repeating units. Nucleosomes. Histone proteins are basic ...