Imaging Technology GroupVisualization, Media and Imaging Laboratory - PowerPoint PPT Presentation

Title: Imaging Technology GroupVisualization, Media and Imaging Laboratory


1
Introduction to the Microscopy Suite
Beckman Institute for Advanced Science and
Technology
February 4, 2003
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
2
Purpose
Provide the Research Community at the Beckman
Institute and the University of Illinois Access
to Microscopy Instrumentation
Beckman Institute for Advanced Science and
Technology

• Purchase
• Training
• Maintenance

Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
3
What makes this work
WebPages
http//www.itg.uiuc.edu/
Helpful support staff
Beckman Institute for Advanced Science and
Technology
Glenn Fried gfried_at_uiuc.edu Karl
Garsha garsha_at_uiuc.edu Scott Robinson
sjrobin_at_uiuc.edu
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.UIUC.edu Microsc
opy Suite
4
Light Microscopes Dissecting Microscopes Reflecte
d light Microscope Stereology Workstation Fluoresc
ence Microscope Multiphoton Laser Scanning
Confocal Microscope
Electron Microscopes Transmission Electron
Microscope Environmental Scanning Electron
Microscope
Scanning Probe Microscopes Nanoscope
IIIa Bioscope Near Field Scanning Optical
Microscopes
Beckman Institute for Advanced Science and
Technology
Wet lab, printing and developing darkrooms,
carbon evaporator, metal evaporator, sputter
coater, tissue processor, critical point dryer,
ultramicrotome, fiber puller
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
5
Dissecting Microscope (Stereoscope)
Fluorescent illumination source Photoshop
software, Kodak DC 290 camera
Transgenic mouse brain with multiple brain tumors
(dark blue). Image courtesy of Ed Roy,
Department of Psychology
Beckman Institute for Advanced Science and
Technology
View of tactile spine sensors used in the control
of insect locomotion. Image courtesy of John M.
Hart, Beckman Institute.
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
6
Fluorescence microscope
Phase contrast, DIC, Laser tweezers, MCID
software, time-lapse video recording, stage
heater, motorized stage, shutters to minimize
light exposure.
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
7
Stereology Workstation
Stereoinvestigator
Estimation of the surface area per unit volume
using cycloid probe.
Beckman Institute for Advanced Science and
Technology
Neurolucida
barrel field neuron
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
8
Beckman Institute for Advanced Science and
Technology
Leica SP2 Multiphoton Laser Scanning Confocal
Microscope
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
9
Reconstruction of neuron
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
10
Spectral Imaging of Polymers
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
11
Two-photon Microscopy
Nsp
Nsp2
s1x10-16
s1x10-50
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
12
Environmental Scanning Electron Microscope
Resolution 2 nm
Normal (HiVac) or Wet mode Peltier-cooled
stage 1000 and 1500C hot stages EDAX Electrical
and mechanical feed throughs Bug spinner Micro
injector Backscattered electron detectors
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
13
Transmission Electron Microscope
Resolution 0.2 nm
200 kV LaB6 gun, TVIPS 2kx2k digital
camera. High tilt stage, rotation stage, cryo
(LN2) stage, tensile testing stage.
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
14
Bioscope
Contact, Tapping Mode, Magnetic force, Electric
force, Phase imaging, Pulsed force mode.
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
15
Nanoscope IIIa
Contact, Tapping Mode, Magnetic force, Electric
force, Phase imaging, Pulsed force mode. Piezo
scanner with an x, y range of 125 microns and 5
micron vertical range. Liquid cell.
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
16
Near Field Scanning Optical Microscope (NSOM)
Beckman Institute for Advanced Science and
Technology
Digital Instruments controller, closed loop x,y
stage, inverted Zeiss microscope, Monochromator
and CCD for spectroscopy. Transmission,
reflection, and collection NSOM. Localized
spectroscopy. Spatially and spectrally resolved
images
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
17
PerkinElmer Spectrum Spotlight
Beckman Institute for Advanced Science and
Technology
Map spectral features from a collection of mid IR
spectra collected from each 6um x 6um pixel.
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
18
Ancillary Equipment
Carbon evaporator Dual source metal
evaporator Sputter coater Tissue processor Ultra
microtome Critical point dryer Fiber
puller Laminar flow hood Glow-discharge unit
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite
19
www.itg.uiuc.edu
Beckman Institute for Advanced Science and
Technology
Imaging Technology Group Visualization, Media
and Imaging Laboratory www.itg.uiuc.edu Microsc
opy Suite