Construction of GatewayTM-based vectors for high-throughput cloning and (co-)expression screening in Escherichia coli Didier Busso, Loubna Salim, Edouard Troesch ...
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All vectors are derived from the pET22b vector (Merck - Novagen) ... An aliquot of eluted fraction (10 l) was loaded onto SDS-PAGE. ORF-His6. GST-ORF-His6 ...
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