Counterstain PowerPoint PPT Presentations

1. Use the appropriate fixative for your sample 2. Choose the right detergent to stain your target. 3. Use appropriate controls. 4. optimize antibody dilution and cell density. 5. Use blocking serum from a different organism than the primary antibody. 6. Primary and secondary antibody optimization. 7. Multiple staining steps (optional). 8. Employ counterstains to help identify cellular landmarks. 9. Get a proper sample mounting?

Gram Staining Lab 3 Hans Christian Gram Developed Gram Staining in 1844 The reagents Primary stain: Crystal violet Mordant: Iodine Selective Treatment ...

Bacterial Staining Endospores Endospore Structure Function Location Bacteria Endospore Location Simple Stain Endospore Stain Differential stain Endospore vs. Spore ...

Supplementary Information The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus Manuel J. Deutsch, Elisabeth ...

An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies ... projections of five to seven optical sections obtained by multiphoton microscopy. ...

... You do heat the to ensure that it does not wash off during the staining process 2 -Stain with crystal violet for one ... Design 1 ...

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Title: PowerPoint Presentation Author: MALACHOWSKYK Last modified by: MALACHOWSKY,KEN Created Date: 3/28/2002 6:37:25 PM Document presentation format

Verhoeff's Elastic Stain: collagen in the dermis is stained red, cell nuclei are ... Alcian Blue (pH 2.5) Stain: Hyaluronic acid in the dermis is stained blue. ...

You should consider osteopathic well care if you wish to receive more than conventional medicine can offer. Dr Bhavik Patel can provide more information.

Why do we care about capsules

Two research version Leica/Bond autostainers are now ... Leica/Bond Autostainers. NALP5; Bond AP polymer kit. Double staining: TAU (DAB) & npTAU (AP) ...

If the bacteria have been removed from a culture on solid media it will have to ... which contrasts markedly with the blue-black colour of the Gram-positive cells. ...

Preparation and Staining of Specimens Often specimens must be fixed and stained to enhance their visibility and to distinguish morphological properties Fixation ...

Title: Label the following parts of the cell: Author: Carol Ward Last modified by: cward Created Date: 9/7/2004 11:15:46 AM Document presentation format

acid-fast stain Culture and stain of fungus Content 1.acid-fast stain of M.tuberculosis 2. Culture of fungus 3.Simple stain of fungus acid-fast stain of M ...

... kuman yang terisolir Smear fiksasi pewarnaan Types of stain Simple stains Differential stains Special stains General stains Pewarnaan sederhana ...

Title: Slide 1 Author: HDesk Last modified by: HDesk Created Date: 8/17/2005 2:11:07 PM Document presentation format: On-screen Show Company: Wesleyan College

Gram staining Types of staining Simple Bacteria stained to contrast environment Differential Bacteria differing chemically react differently to staining Gram stain ...

Title: PowerPoint Presentation Author: S1S Last modified by: S1S Created Date: 10/16/2004 10:31:20 PM Document presentation format: On-screen Show Company

What bacterium ferments mannitol and has a high tolerance for salt? Do any other organisms tolerate salt? * * Title: Slide 1 Author: Patricia Sidelsky Last modified by:

Title: Streptococci Author: Robin Smith Last modified by: SSC Created Date: 3/2/2000 1:16:42 PM Document presentation format: (3:4)

Differential stain uses two dyes, also classifies bacteria into ... have an outer membrane (lipoprotein, lipopolysaccharide) and a thin layer of peptidoglycan) ...

Retrospective Dosimetry by Translocation Analysis Lecture Module 5

Microbe are involved in. nutrient production & energy flow. decomposition. production of ... nutrient broth liquid medium containing beef extract & peptone ...

Bacterial species with walls containing small amounts of peptidoglycan and, ... 1 whooping cough, Bordetella pertussis. 2. typhoid, Salmonella typhi ...

8. Rinse the with distilled water. GRAM STAIN PROCEDURE ... 10. Rinse the with distilled water. GRAM STAIN PROCEDURE ...

Observing Microorganisms through a Microscope Chapter 3 Basic techniques needed to study Bacteria 1. Grow Bacteria 2. Isolate Bacteria 3. Grow Bacteria in pure culture 4.

Introduction to Lab: Differential Stains Gram Staining Introduction to Lab Differential Stains Gram Staining Basic classification of bacteria is based on the cell ...

how prepare bacterial smear, and types of staining

Archaebacteria and Eubacteria

Preparing a Smear Exercise 10

Review for Lab Practical #1 BIOL2421L Dr. Ann M. Davis Mueller-Hinton Tellurite (MHT) Agar Differential Special features: Tellurite (metal) Used for: Distinguishing ...

Ziehl Neelsen (ZN) heat method. Acid fast cells have higher ... Vegetative cells prior to sporulation- red. Eosin Methylene Blue Agar. Selective Media ...

Microbiology by Nester, Anderson, Roberts, Pearsall & Nester (5rd edition) ... MYCOLOGY - fungi and yeast. PROTOZOOLOGY - protozoa. PARASITOLOGY - parasites ...

... Streptococcal pharyngitis. scarlet fever. rheumatic fever. necrotizing fasciitis 'flesh ... S. aureus: upper respiratory tract & 'typical' pneumonia ...

Archaebacteria and Eubacteria Sarita Bhatia Department of Botany, DAVCG, Yamunanagar.

Archaebacteria and Eubacteria Sunita Kaushik Department of Botany, DAVCG, Yamunanagar.

Acid Fast Staining Detects the high mycolic acid content of certain bacterial cell walls, like those of Mycobacteria Mycolic acids are long fatty acids found in ...

USING TABLET PCs IN MICROBIOLOGY LECTURE. HP'S TECHNOLOGY FOR TEACHING GRANT ... Please pick up your tablet from the cart in the front of the room ...

Nipah virus is an enveloped, nonsegmented,negative-stranded RNA ... ????????? Nipah virus. ???????????????????????????????? vero E6 cells ... ???? herringbone ...

Classification of bacteria DR.THAMINA SAYYED REGISTRAR MICROBIOLOGY KKUH Bacterial cells Classification System 3 Domains 1978 Carl Woese 1.

Introduction to Lab Ex. Differential Stains Gram Staining Gram-positive cell walls Gram-negative cell walls Thick peptidoglycan 90% peptidoglycan Teichoic acids 1 ...

MICROSCOPY AND STAINING CHAPTER 3 Metric Units Light Properties Wavelength polarity Waves can be added Light Properties Resolution Wavelength/Resolution Interaction ...

Often important in making vaccines-why? ... Obligate aerobe eg Pseudomonas aeruginosa. Obligate anaerobe eg Clostridium botulinum ...

Title: Foundations in Microbiology Author: Laraine Powers Last modified by: Trey Townsend Created Date: 8/15/2003 7:33:38 PM Document presentation format

DEMONSTRATION OF IRON (Perls's Prussian Blue reaction) The majority of non-haem iron is stored as haemosiderin (a ferric iron-protein complex), a small amount as ...

... of some cells to resist destaining (decolor) with alcohol. 4 reagents ... old cultures, overheating at fixation step, decolor to long or rinsed to long-water ...

WikiNeuron: Semantic Neuro-Mashup http://neuroweb3.med.yale.edu/mediawiki/index.php/WikiNeuron Kei Cheung Yale Center for Medical Informatics

Three test reactions are added together at a time to give a 7-digit number, ... of a total of 15 biochemical tests as well as an enclosed inoculating wire.

... agent for tuberculosis) easy to recognize in sputum, amidst numerous organisms. sputum sample containing. M. tuberculosis. Special Stains ...

TESTING OF FIELD SPECIMENS FOR WEST NILE AND OTHER ARBOVIRUSES ... Second antibody = FITC labeled anti-mouse IgM. West Nile Virus Positive Crow Kidney 107A ...

PATHOPHYSIOLOGY PATHOPHYSIOLOGY DEFINED Involves the study of function that results from disease processes. PATHOPHYSIOLOGY What is pathology?

Slide 1 ... Stem Cells

Following axotomy, animals treated with BDNF show less AR down ... guinea pig-Cy5 (Jackson Immuno) , and donkey anti-sheep-Texas Red (Jackson) ...

Medical Microbiology Bacteria