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Vectors - Plasmids such as pMB1 and ColE1 (multicopy plasmids) replicate in a 'relaxed' fashion ... array of restriction endonuclease sites in a very short expanse ...

Plasmids such as pMB1 and ColE1 (multicopy plasmids) replicate in a 'relaxed' fashion ... array of restriction endonuclease sites in a very short expanse ...

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PowerPoint Presentation PowerPoint PPT Presentation

PowerPoint Presentation - ori pRi kanr pTF101.1::Ri 10.1 kbp ori RK2 (incP ) RB 2x P35S TEV enhancer bar ori pVS1 Tvsp pTF101.1 9.2 kbp LB aadA pgl ori colE1 ori RK2 (incP ) pE2759 30.1 kbp

ori pRi kanr pTF101.1::Ri 10.1 kbp ori RK2 (incP ) RB 2x P35S TEV enhancer bar ori pVS1 Tvsp pTF101.1 9.2 kbp LB aadA pgl ori colE1 ori RK2 (incP ) pE2759 30.1 kbp

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Cloning - Cloning Using Plasmid Vectors Vector = a molecule used as a vehicle to carry foreign DNA into a host cell Simplest vector = plasmid Features of Plasmids Size ...

Cloning Using Plasmid Vectors Vector = a molecule used as a vehicle to carry foreign DNA into a host cell Simplest vector = plasmid Features of Plasmids Size ...

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Co-expression with Duet vectors PowerPoint PPT Presentation

Co-expression with Duet vectors - Co-expression with Duet vectors Lab of Molecular Genetics Yong-wook, Choi (2003. 4. 10) Co-expression in E.coli Multi-component protein Important in many cellular ...

Co-expression with Duet vectors Lab of Molecular Genetics Yong-wook, Choi (2003. 4. 10) Co-expression in E.coli Multi-component protein Important in many cellular ...

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Saccharomyces cerevisiae PowerPoint PPT Presentation

Saccharomyces cerevisiae - Saccharomyces cerevisiae : Saccharomyces cerevisiae is commonly known as

Saccharomyces cerevisiae : Saccharomyces cerevisiae is commonly known as

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Protein2: Properties PowerPoint PPT Presentation

Protein2: Properties - Title: Bio 101 Net 1 Author: George Church Last modified by: George Church Created Date: 5/6/2000 1:28:20 AM Document presentation format: On-screen Show

Title: Bio 101 Net 1 Author: George Church Last modified by: George Church Created Date: 5/6/2000 1:28:20 AM Document presentation format: On-screen Show

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Bio%20101:%20Genomics%20 PowerPoint PPT Presentation

Bio%20101:%20Genomics%20 - Tue Sep 18 Intro 1: Computing, statistics, Perl, Mathematica ... 'The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes. ...

Tue Sep 18 Intro 1: Computing, statistics, Perl, Mathematica ... 'The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes. ...

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Lecture 2 catch up PowerPoint PPT Presentation

Lecture 2 catch up - Lecture 3 Lecture 2 catch up Vector structure Copy number control Selectable marker Plasmid DNA isolation ESTs DNA sequencing Entering the Honeybee database

Lecture 3 Lecture 2 catch up Vector structure Copy number control Selectable marker Plasmid DNA isolation ESTs DNA sequencing Entering the Honeybee database

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Addressable Bacterial Conjugation PowerPoint PPT Presentation

Addressable Bacterial Conjugation - Advisors: Professors Adam Arkin and Jay Keasling. GSIs: Chris Anderson and John Dueber ... Samantha Liang. Bryan Hernandez. Kaitlin A. Davis. Daniel Kluesing ...

Advisors: Professors Adam Arkin and Jay Keasling. GSIs: Chris Anderson and John Dueber ... Samantha Liang. Bryan Hernandez. Kaitlin A. Davis. Daniel Kluesing ...

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Genetic Manipulation and Bacterial Genetics' M J Larkin PowerPoint PPT Presentation

Genetic Manipulation and Bacterial Genetics' M J Larkin - xt 2288 (Belfast 90972288 Diverted to the DKB) DKB Questor ... Pigeon hole in Biology & Biochemistry office. ALWAYS LEAVE MESSAGE and HOW TO CONTACT YOU! ...

xt 2288 (Belfast 90972288 Diverted to the DKB) DKB Questor ... Pigeon hole in Biology & Biochemistry office. ALWAYS LEAVE MESSAGE and HOW TO CONTACT YOU! ...

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Plant Expression System PowerPoint PPT Presentation

Plant Expression System - Overview of methods for plant transformation ... produces filaments resembling the pili used for interbacterial conjugation.(VirB) ...

Overview of methods for plant transformation ... produces filaments resembling the pili used for interbacterial conjugation.(VirB) ...

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Restriction endonucleases and DNA methyltransferases from Haemopilus influenzae and Neisseria gonorrhoeae PowerPoint PPT Presentation

Restriction endonucleases and DNA methyltransferases from Haemopilus influenzae and Neisseria gonorrhoeae - Restriction endonucleases and DNA methyltransferases from Haemopilus influenzae and Neisseria gonorrhoeae Dr Andrzej Piekarowicz Institute of Microbiology

Restriction endonucleases and DNA methyltransferases from Haemopilus influenzae and Neisseria gonorrhoeae Dr Andrzej Piekarowicz Institute of Microbiology

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Caenorhabditis elegans as a model for Staphylococcus aureus pathogenesis PowerPoint PPT Presentation

Caenorhabditis elegans as a model for Staphylococcus aureus pathogenesis - Caenorhabditis elegans as a model for Staphylococcus aureus pathogenesis Jakob Begun Ausubel Lab - MGH

Caenorhabditis elegans as a model for Staphylococcus aureus pathogenesis Jakob Begun Ausubel Lab - MGH

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Setting up a transformation--how will the competent cells be treated? PowerPoint PPT Presentation

Setting up a transformation--how will the competent cells be treated? - Isolate phage (and single-stranded DNA) in supernatant ... is packaged and extruded into supernatant--can then be isolated for sequencing, etc. ...

Isolate phage (and single-stranded DNA) in supernatant ... is packaged and extruded into supernatant--can then be isolated for sequencing, etc. ...

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Replicaci - Inicio de la replicaci n en pl smidos dependientes de prote na iniciadora. DnaA ... Estabilidad plasmidica: una herencia justa. Recombinaci n. Sitio-espec fica ...

Inicio de la replicaci n en pl smidos dependientes de prote na iniciadora. DnaA ... Estabilidad plasmidica: una herencia justa. Recombinaci n. Sitio-espec fica ...

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???????? ????????? pBS72 ??? ?????? ??? ???????? ????????? ?????? PowerPoint PPT Presentation

???????? ????????? pBS72 ??? ?????? ??? ???????? ????????? ?????? - , 1, Dna ...

, 1, Dna ...

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Plasmids and Plasmid Biology PowerPoint PPT Presentation

Plasmids and Plasmid Biology - Plasmids and Plasmid Biology 1. Plasmid structure 2. Plasmid replication and copy number control 3. Plasmid transfer 4. Plasmids as tools 5. F plasmids

Plasmids and Plasmid Biology 1. Plasmid structure 2. Plasmid replication and copy number control 3. Plasmid transfer 4. Plasmids as tools 5. F plasmids

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Using%20Logical%20Circuits%20to%20Analyze%20and%20Model%20Genetic%20Networks PowerPoint PPT Presentation

Using%20Logical%20Circuits%20to%20Analyze%20and%20Model%20Genetic%20Networks - Title: Evolution of Complex Dynamics and the Inverse Problem in Genetic Networks Author: ICC Last modified by: ICC Created Date: 4/29/2005 8:37:23 PM

Title: Evolution of Complex Dynamics and the Inverse Problem in Genetic Networks Author: ICC Last modified by: ICC Created Date: 4/29/2005 8:37:23 PM

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Plasmid DNA - Plasmid DNA

Plasmid DNA

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EXU Workshop Modern Methods in Molecular Biology PowerPoint PPT Presentation

EXU Workshop Modern Methods in Molecular Biology - The PCR technique was patented by Cetus Corporation, where Mullis worked when he ... ?DE3 lysogen: lacUV5-T7 RNA pol; IPTG inducible. pLysS. T7 lysosyme ...

The PCR technique was patented by Cetus Corporation, where Mullis worked when he ... ?DE3 lysogen: lacUV5-T7 RNA pol; IPTG inducible. pLysS. T7 lysosyme ...

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Enzymes for manipulating DNA PowerPoint PPT Presentation

Enzymes for manipulating DNA - Enzymes for manipulating DNA *** Buffers and solution conditions*** I. DNA polymerases III. Kinase and alkaline phosphatase IV. Nucleases V. Topoisomerase

Enzymes for manipulating DNA *** Buffers and solution conditions*** I. DNA polymerases III. Kinase and alkaline phosphatase IV. Nucleases V. Topoisomerase

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Plant Biotechnology and GMOs PowerPoint PPT Presentation

Plant Biotechnology and GMOs - Plant Biotechnology and GMOs Chapter 14 (Plus other bits and bobs) And then?..... What is the last step?..... Tissue culture The basics!

Plant Biotechnology and GMOs Chapter 14 (Plus other bits and bobs) And then?..... What is the last step?..... Tissue culture The basics!

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Genentech Founders PowerPoint PPT Presentation

Genentech Founders - Title: Author: xulei Last modified by: Created Date: 3/3/2000 9:10:41 AM Document presentation format: (4:3) Company

Title: Author: xulei Last modified by: Created Date: 3/3/2000 9:10:41 AM Document presentation format: (4:3) Company

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DNA Replication I: Basic Mechanism and Enzymology PowerPoint PPT Presentation

DNA Replication I: Basic Mechanism and Enzymology - Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...

Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...

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A Different Drummer PowerPoint PPT Presentation

A Different Drummer - A Different Drummer 19th Century Social Reform Henry David Thoreau & Ralph Waldo Emerson Standard 8.6.7 Identify common themes in American art as well as ...

A Different Drummer 19th Century Social Reform Henry David Thoreau & Ralph Waldo Emerson Standard 8.6.7 Identify common themes in American art as well as ...

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MALDIToF Mass Spectrometry of PowerPoint PPT Presentation

MALDIToF Mass Spectrometry of - Dept. of Chemistry, University of New Orleans, New Orleans, LA ... Elution solution changed from 0.3 N NH4OH (aq) to 0.3 N NH4OH in 1:1 MeOH:ACN ...

Dept. of Chemistry, University of New Orleans, New Orleans, LA ... Elution solution changed from 0.3 N NH4OH (aq) to 0.3 N NH4OH in 1:1 MeOH:ACN ...

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Produktion humaner Proteine in Bakterien PowerPoint PPT Presentation

Produktion humaner Proteine in Bakterien - process optimisation. Monitoring of metabolic load. Control of plasmid copy number ... KEY ASPECTS for the OPTIMIZATION of RECOMBINANT PROTEIN PRODUCTION on INDUSTRIAL ...

process optimisation. Monitoring of metabolic load. Control of plasmid copy number ... KEY ASPECTS for the OPTIMIZATION of RECOMBINANT PROTEIN PRODUCTION on INDUSTRIAL ...

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Development of Anatomically Realistic Numerical Breast Phantoms with Accurate Dielectric Properties PowerPoint PPT Presentation

Development of Anatomically Realistic Numerical Breast Phantoms with Accurate Dielectric Properties - MR image processing: Homomorphic filtering. Coronal image before filtering ... Additional structural development: Skin and chestwall. Elliptical mask. Smooth ...

MR image processing: Homomorphic filtering. Coronal image before filtering ... Additional structural development: Skin and chestwall. Elliptical mask. Smooth ...

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Grids in Biology: Bioinformatics and Medicine - Grids in Biology: Bioinformatics and Medicine

Grids in Biology: Bioinformatics and Medicine

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Vectores y su Origen PowerPoint PPT Presentation

Vectores y su Origen - ... en su seno varios genes, a menudo responsables de car cter sticas tiles para la ... A veces, en presencia de un pl smido conjugativo, otro pl smido puede ...

... en su seno varios genes, a menudo responsables de car cter sticas tiles para la ... A veces, en presencia de un pl smido conjugativo, otro pl smido puede ...

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Bovine Prolactin Receptor PRLR Gene in expressing Bovine Growth Hormone BGH PowerPoint PPT Presentation

Bovine Prolactin Receptor PRLR Gene in expressing Bovine Growth Hormone BGH - Lower temperature was chosen as the annealing temperature for PCR= 58.51 C ... so that later it can anneal and ligate to our DNA of interested by using ligase ...

Lower temperature was chosen as the annealing temperature for PCR= 58.51 C ... so that later it can anneal and ligate to our DNA of interested by using ligase ...

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Introduction to Microbial Genetics PowerPoint PPT Presentation

Introduction to Microbial Genetics - Alfred Hershey and Martha Chase and the Blender Experiment. Hershey and Chase wanted to verify that DNA was the hereditary material ...

Alfred Hershey and Martha Chase and the Blender Experiment. Hershey and Chase wanted to verify that DNA was the hereditary material ...

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Molecular Genetics PCB4522 Spring 2004 Lecture 5Replicationpart D Dr' Eva CzarneckaVerner PowerPoint PPT Presentation

Molecular Genetics PCB4522 Spring 2004 Lecture 5Replicationpart D Dr' Eva CzarneckaVerner - 1. Single type of catalytic subunit (dnaE) used in replication of ... 'Ice-skating' Interactions with DNA via water molecules. Replication of E. coli chromosome ...

1. Single type of catalytic subunit (dnaE) used in replication of ... 'Ice-skating' Interactions with DNA via water molecules. Replication of E. coli chromosome ...

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Tema 5 - ... (degradaci n de tolueno, xileno, alcanfor, etc.) en Pseudomonas. Inducci n de tumores en plantas (pl smido Ti de Agrobacterium tumefaciens).

... (degradaci n de tolueno, xileno, alcanfor, etc.) en Pseudomonas. Inducci n de tumores en plantas (pl smido Ti de Agrobacterium tumefaciens).

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Chapter4 Heredity and variation of bacteria PowerPoint PPT Presentation

Chapter4 Heredity and variation of bacteria - ... bacteriophage gal bio gal bio gal bio gal bio bio gal specialized transduction lysogenic conversion DNA recombination protoplast fusion The Ames Test for ...

... bacteriophage gal bio gal bio gal bio gal bio bio gal specialized transduction lysogenic conversion DNA recombination protoplast fusion The Ames Test for ...

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Using Logical Circuits to Analyze and Model Genetic Networks PowerPoint PPT Presentation

Using Logical Circuits to Analyze and Model Genetic Networks - Introduction to logical models history and application ... Repressor B. On' Off' Construction of the plasmid. Gardner, Cantor & Collins (2000) ...

Introduction to logical models history and application ... Repressor B. On' Off' Construction of the plasmid. Gardner, Cantor & Collins (2000) ...

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METHODS IN MOLECULAR BIOLOGY PowerPoint PPT Presentation

METHODS IN MOLECULAR BIOLOGY - Ligation of compatible sticky ends. 5'-A-C-G-G-T-A-C-T-A-G A-A-T-T-C-A-G-C-T-A-C-G-3' ... Removal of 5'-PO4 Prevents Vector Self Ligation. Purification of Plasmids ...

Ligation of compatible sticky ends. 5'-A-C-G-G-T-A-C-T-A-G A-A-T-T-C-A-G-C-T-A-C-G-3' ... Removal of 5'-PO4 Prevents Vector Self Ligation. Purification of Plasmids ...

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Introduction to Microbial Genetics - A purine is bonded to a complementary pyrimidine Bases ... tRNA tRNAs attach to a specific amino acid and carry it to the ribosome There are 20 amino acids 61 ...

A purine is bonded to a complementary pyrimidine Bases ... tRNA tRNAs attach to a specific amino acid and carry it to the ribosome There are 20 amino acids 61 ...

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Recombinant DNA Technology PowerPoint PPT Presentation

Recombinant DNA Technology - SSR Genetic Markers . Microsatellites (Simple Sequence Repeats: SSRs). Used for mapping the human genome--the main marker system used today. SSRs are short ...

SSR Genetic Markers . Microsatellites (Simple Sequence Repeats: SSRs). Used for mapping the human genome--the main marker system used today. SSRs are short ...

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Sistemas de modificaci PowerPoint PPT Presentation

Sistemas de modificaci - M todos f sicos Microinyecci n Microinyecci n Biolistic particle delivery Electroporaci n Electroporaci n Magnetofecci n MATra ...

M todos f sicos Microinyecci n Microinyecci n Biolistic particle delivery Electroporaci n Electroporaci n Magnetofecci n MATra ...

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Functional Genomics: Progress with Physiology and Genetic Tools PowerPoint PPT Presentation

Functional Genomics: Progress with Physiology and Genetic Tools - Deletion cassettes generated to date. Recombination Event 1 ... Generate deletions from mutagenic cassettes for further analysis by all groups ...

Deletion cassettes generated to date. Recombination Event 1 ... Generate deletions from mutagenic cassettes for further analysis by all groups ...

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Nucleic acid chemistry part 3 : DNA replication - Nucleic acid chemistry part 3 : DNA replication

Nucleic acid chemistry part 3 : DNA replication

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Molecular Genetics PCB4522 Spring 2004 Lecture 3 Replication part B Dr' Eva CzarneckaVerner PowerPoint PPT Presentation

Molecular Genetics PCB4522 Spring 2004 Lecture 3 Replication part B Dr' Eva CzarneckaVerner - Plasmid incompatibility is connected to the regulation of copy number and ... there is no free end; linear mitochondrial DNA of Paramecium crosslinks the ends. ...

Plasmid incompatibility is connected to the regulation of copy number and ... there is no free end; linear mitochondrial DNA of Paramecium crosslinks the ends. ...

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Kein Folientitel PowerPoint PPT Presentation

Kein Folientitel - Metabolic and engineering integrated approach for the optimisation of ... Institute of Applied Microbiology, University of Agricultural Sciences Vienna ...

Metabolic and engineering integrated approach for the optimisation of ... Institute of Applied Microbiology, University of Agricultural Sciences Vienna ...

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docmutin - cloning

cloning

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Evolution of Complex Dynamics in Mathematical and Electronic Models of Genetic Networks PowerPoint PPT Presentation

Evolution of Complex Dynamics in Mathematical and Electronic Models of Genetic Networks - Glass, Kauffman, Pasternack, 1970s. Rationale for the ... Perkins, Hallett, Glass (2004) ... (Perkins, Jaeger, Reinitz, Glass, PLOS Computational Biology 2006) ...

Glass, Kauffman, Pasternack, 1970s. Rationale for the ... Perkins, Hallett, Glass (2004) ... (Perkins, Jaeger, Reinitz, Glass, PLOS Computational Biology 2006) ...

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V28 Synthetic Biology PowerPoint PPT Presentation

V28 Synthetic Biology - Synthetic biologists engineer complex artificial biological systems to ... into six sections, alpha through z ta, using five restriction sites unique ...

Synthetic biologists engineer complex artificial biological systems to ... into six sections, alpha through z ta, using five restriction sites unique ...

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Prsentation PowerPoint PowerPoint PPT Presentation

Prsentation PowerPoint - Co-expression is mandatory. Co-expression. in E. coli or in insect cells ... They are rod-shaped (latin baculum = stick), 40-50 nm in diameter and 200-400 nm ...

Co-expression is mandatory. Co-expression. in E. coli or in insect cells ... They are rod-shaped (latin baculum = stick), 40-50 nm in diameter and 200-400 nm ...

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Cours 3L5BG1M PowerPoint PPT Presentation

Cours 3L5BG1M - Woese en 1987, utilise un nombre important de s quences d'ARN ribosomique. ... Taille allant de 580 kb pour Mycoplasma genitalium 9120 kb pour Streptomyces ...

Woese en 1987, utilise un nombre important de s quences d'ARN ribosomique. ... Taille allant de 580 kb pour Mycoplasma genitalium 9120 kb pour Streptomyces ...

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Informationsverarbeitung%20in%20Bakterien PowerPoint PPT Presentation

Informationsverarbeitung%20in%20Bakterien - Assay of LacZ activities on. plates and in liquid media. Promoter ... ori colE1. IR. tnp. BcuI. ldh. MPN673 -10 -35 -35 -10 81 -160 81 -118 81 -84 81 -53 ...

Assay of LacZ activities on. plates and in liquid media. Promoter ... ori colE1. IR. tnp. BcuI. ldh. MPN673 -10 -35 -35 -10 81 -160 81 -118 81 -84 81 -53 ...

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