Agar diffusion method Basmah almaarik - PowerPoint PPT Presentation
Title:
Agar diffusion method Basmah almaarik
Description:
Lab # 2 Method 1) Broth dilution 2) Agar diffusion (solid) -a) Kirby-Bauer test (= disc test) - b) Stock s methods Fresh organism suspended in broth. – PowerPoint PPT presentation
Number of Views:109
Avg rating:3.0/5.0
Title: Agar diffusion method Basmah almaarik
1
Agar diffusion methodBasmah almaarik
- Lab 2
2
Antibiotic Sensitivity Test
- Method
- 1) Broth dilution
- 2) Agar diffusion (solid)
- -a) Kirby-Bauer test ( disc test)
- - b) Stocks methods
3
a) Kirby-Bauer Test (disc diffusion test) (Solid
agar test)
- Fresh organism suspended in broth.
- (0.5 MaCfaraland standard)
- Swab organism all over the plate evenly.
- Place discs containing specified concentration of
different antibiotics on the plate.
4
Kirby-Bauer Test
- Incubate 37C x 24hr
- Measure diameter of Clear zone around the disc
. (Inhibition zone) - Use tables to assess if zone size indicates
- resistance or sensitivity to that antibiotic
5
Kirby-Bauer Test (disc diffusion test)
6
(No Transcript)
7
How to Streak the sensitivity plate
- Immerge swab then remove excess by pressing
against wall of tube - Do one line in the center of plate by swab then
spread evenly - Add antibiotic disks using needle
- Always work near flame to avoid contamination
- Dont put disk near the edge
8
B) Stockes Method
- Interpretation based on comparison between
- zones seen with the test organism those of the
known sensitive control.
Q.C organism
Antibiotic disk
Test organism
9
Results of Stockes Method
- T gt C or T C ? Sensitive
- Tlt C ? Resistance
Test organism
Control organism
10
Antibiotic Dispenser (Disk Dispenser)
http//rfdp.seafdec.org.ph/publication/manual/anti
biotics/chapter2.html
11
Antibiotic ring
12
Automatic colony counting and zone measuring
13
Work done by students
- Prepare 2 Kirby-Bauer technique plates
- Using disc dispenser
- Using antibiotic ring technique
- Prepare 1 stocks method plate using control and
test organisms given to you and inoculate 3
antibiotics using the needle. - Next day measure the inhibition zone in each
plate and determine whether its sensitive or
resistance by Zone Diameter Interpretative
Standards
14
- Thank You
Recommended
CrystalGraphics Presentations
