Common%20Laboratory%20Glassware%20and%20Volumetric%20Glassware - PowerPoint PPT Presentation

Title: Common%20Laboratory%20Glassware%20and%20Volumetric%20Glassware


1
Common Laboratory Glassware and Volumetric
Glassware
Prepared By Michigan Department of Environmental
Quality Operator Training and Certification Unit
2
GLASSWARE
Graduated Cylinder
Griffin Beaker
Erlenmeyer Flask
Graduated Pipet
3
GLASSWARE
Approximate Graduations
Griffin Beaker
4
GLASSWARE
Approximate Graduations
Erlenmeyer Flask
5
GLASSWARE
Graduated Cylinder
Used for Routine Volume Measurements of 25 mL or
Greater
6
GLASSWARE
Wide Tip Use When Solids in Sample
Used for Routine Volume Measurements of 25 mL or
Less
Graduated Pipet
7
GLASSWARE
Wide Tip Use When Solids in Sample
NOT VOLUMETRIC
Graduated Pipet
8
Volumetric Glassware
Required for High Accuracy and Precision
Required When Preparing or Using Standard
Solutions
9
Volumetric Glassware
Accuracy and Precision
Standard Solutions
Volumetric Flask
Buret
Volumetric Transfer Pipet
10
VOLUMETRIC GLASSWARE
Must Be Used Properly
11
BURET
12
BURET
To Deliver
Calibration Temperature
13
Rinse With Titrant
Completely Coat Inside Surface
14
Beads of Liquid
Not Clean
15
Rinse Three Times
Fill to Above Top Graduation
16
Discharge All Air Bubbles
17
Drain Until Top of Liquid Is In Graduations
18
Curved Surface of Liquid
19
MENISCUS READER
20
Proper Reading
X
O
X
21
Bottom of Meniscus to Top of Graduation
22
Ten Graduations
Increase

23
24.16
9.64
24
Proper Grip
Record Initial Reading
Add Indicator
25
Mix Well
Near Endpoint
26
Split Drops
Transfer To Flask
Rinse Into Reaction
27
At Endpoint
Record Final Reading
Read
Final Reading Initial Reading Volume
Delivered
28
Properly Dispose Excess
Properly Clean
Air Dry
29
Proper Storage
30
Volumetric Transfer Pipets
31
Volumetric Transfer Pipets
One Calibration
To Deliver
Calibration Temperature
32
Fill By Vacuum
33
Fill By Vacuum
Rinse
Pipet Bulb
34
Must Drain Evenly
Not Clean
35
Wipe Tip
Touch Container To Remove Drop
Fill Above Calibration
Drain To Calibration
36
Some Will Remain In Tip
Allow To Drain
Touch Tip To Flask
37
Exception
ETCHED Ring
NOT Painted
FOR BLOW-OUT
38
Air Dry
Properly Clean
39
Using Volumetric Pipets
1. Rinse with solution to be pipeted
(Observe for even draining)
2. Fill to above graduation
3. Wipe off outside of pipet
4. Drain to graduation
( bottom of meniscus to top of graduation)
5. Touch tip to waste vessel
6. Transfer by gravity
(vertical - dont force)
7. Touch tip to inside of receiving flask
(clean pipet)
40
Volumetric Flasks
41
To Contain
TC
NOT Intended To Deliver
42
To Prepare Solutions With Accurately Known
Concentration
43
Fill Drop-wise
To Calibration
Fill Half And Mix
Fill To Below Graduation
44
Invert Rapidly
45
Properly Clean
Transfer To Reagent Bottle
Air Dry
46
Volumetric Glassware
Accuracy and Precision
Standard Solutions
Volumetric Flask
Buret
Volumetric Transfer Pipet
47
Common Laboratory Glassware and Volumetric
Glassware
Prepared By Michigan Department of Environmental
Quality Operator Training and Certification Unit