Pectinase Prelab

1
Pectinase Prelab

• Please copy the following definitions (on
  handout)
• Chemical reaction when atoms are rearranged in a
  molecule or molecules.
• Enzyme a protein that speeds up a chemical
  reaction
• Substrate the substance an enzyme changes
  Catalyst anything that speeds up a reaction

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The real chemical name for pectin is
polygalacturose. What is the name of the
monosaccharide it is made of?
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Ga-lac-tur-ose Its the monosaccharide
subunit of poly-galactarose. Science words
always make sense, because theyre designed to be
used by speakers of every language
Check Yourself Theres lots of different kinds
of sugarsout there. Galacturose has 6 carbon
atoms how many oxygen atoms must it have?
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Heres a small part of the long chain
This is the bond well be breaking in the lab!!!
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Pectinase is the protein well be using in the
lab.
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ENZYME
SUBSTRATE
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Pectinase attaches to pectin at the active site
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And then breaks a bond in the polysaccharide!
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Some proteins (like Pectinase) are enzymes.
Different enzymes speed up all different kinds of
reactions in organisms!
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SUBSTRATE
Enzymes are very selective about
which substrates they bind. In other
words, Theyre Specific.
Enzyme
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Apples have lots of pectin
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We will be measuring the effect of pectinase by
measuring how fast liquid from applesauce drips
through a filter.
What effect will chopping pectin into little
pieces have on the amount of juice you collect?
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Homework
Hypothesize!

• Write a hypothesis on page 2 of the lab report
  based on what you know.
• It should answer the question What will
  pectinase do to pectin in applesauce?

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Predict

• Remember what a prediction is An if then
  statement that will test your hypothesis using
  data from the experiment.
• If hypothesis then data you expect
• Write a prediction on your lab report

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Background

• With the time that remains, begin writing your
  background section on page 1 of the lab report.
  Use the bullet points listed to guide your
  response.