?????????????,?????? DE/ME/DAA

1
?????????????

• ??? ??
• ?????????(??)????
• ???????????, ???

2
? ?

• ?????????,????????????????
• ?????????????????????
• ??????????400-500?/?,??????25.
  ????????????,?????
• ??????????????????????(???????????????)??????????
  ???,????????

3
????????????????

• ???????????????????
• ?????????????,??????????????????
• ??????????8???
• ?????????????,?????? DE/ME/DAA
• ???????????????????????
• ?????????????
• ??????????
• ??????????????(HMB)
• ??????????????
• ?????,???????
• ??????????

4
???????-???!

• DE, ME, TAA ? DAA ????,?? gt10!
• ??ME???1-year?????,???????????? 2-4?
• ?????????ME??10,????
• ???????
• ????,???????,??????????,????????????
• ???????
• ????????
• ????, ????,????ME ? TAA????? 5
• ????? (USD 2-5/t)
• ??????????? (USD 3-4/t)

5
??? ????????????
0.50
0.50
Total Batch
100
100
100
100
Nutrients
ME.FOR POULTRY
Kcal/Kg
3000
3000
3000
3000
CRUDE FAT

7.87
9.23
7.87
8.51
LYSINE

1.15
1.15
1.15
1.15
MET CYS

0.85
0.85
0.85
0.85
THREONINE

0.78
0.78
0.78
0.78
PHOSPHORUS
-
AVAIL.

0.40
0.40
0.40
0.40
SODIUM

0.16
0.16
0.16
0.16
Feed Cost
Bht
/kg
8.180
8.280
8.180
8.240
/
-
Bht
/ton
100
60
/
-
/ton
2.40
1.45
6
1. ?????????ME(??lt10 kcal/kg)? DAA
The guy !
7
??? AME????
???????????( NSPs) 0.1.
??????????,???????
????540 kcal/kg
8
???????????????, n28????????3,080,
????3.7(NRC 1994 3,350 INRA 2002 3,130).
China
9
Full-fat Rice Bran(??), n 13Mean AMEn2,453
kcal/kg, CV13.0
??
10
Solvent Extracted Rice Bran(????), n 7mean
AMEn1,850 kcal/kg, CV15.2
11
Cassava chips(???), n 4mean AMEn2,548
kcal/kg, CV13.2
12
Fish meal(??), n 11mean AMEn2,423 kcal/kg,
CV15.2
Peru
13
Soy bean meal(??), n 36mean AMEn2,335
kcal/kg, CV10.7
14
???????? (DE) ???
???
. ??-???????? !
15
?????????? ??????
??????? ????
16
?????????? ??????
???
???
Up to 2 rooms with 120 small cages or 60 large
cages 1 room with either 60 small cages or 48
large cages
17
????????(NIR)??????????
?????????????
???????
?????????,??????????
??????????????
18
??????? - ??????????????
???????????????????????
???,???????????,???????????????????
19
???NIR?????????? - ????????????????
ASA Ring Test Results 1999
NIR Adisseo
US reference
max
US SBM Lysine
Min
3.5
Mean2.80 Min1.94 Max3.16
3.16
NIR
2.89
3
Lysine,
2.5
1.94
2
1.5
VN
TH
TH
TH
SN
SN
MY
MY
MY
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
US
US
???????????(21????)
Analytical difference from mean (Labs 30.9 -
12.9, Adisseo NIR 3.2)
20
????NIR????
Adisseo, SIN
TAA DAA values, back to customers to be used
for diet formulation

• Customer without NIR

• Do in vivo test in France for outlier samples

21
2. ????????????????

• ??????????????,???DAA???,???????,???????1-4?????
  
• ???????????????????????
• ????????,????????????,????????????????????????????
  ??????????2-4?????
• ????????4-16?/??
• ??? NIR???????????2003?

22
Adisseo (1980-2004) ????????????????????

• 1. ?????
• 2.??48??
• 3. ????50 g????
• 4. ??48 h????
• 5. ?????????????
• 6. ?????????????
• 7. ???????????????? 2003

23
? ?DAA??????
Broiler feed 0-21 days
FF/100kg
1
2
3
4
Standard
- 5 aa
- 5 aa - 100 kcal
3 aa adjusted / AME
Total
100
100
100
100
111.98
110.51
108.99
107.61
Cost/100 kg
100
98.7
97.3
96.1
??????? 1- 4
24
3. ??????????(NSP)?

• ????????
• ???????????????65-80? 70-80????,???????????NSPs?
  
• ????????????????????????????
• ??????????
• ???????????? 1980s
• ?????????????????
• ??-??????????90????
• ??????????????
• ?????????

25
??????????????????????????
Barley grain
???
????
IRTA - 1996
26
????????????, 220 g/kg
Arabinogalactans ??????
50-55 Pectins ??
Xylo-galacturonans ?????
Galacturonans ???
Rhamno-galacturonans a-???
20-30 Cellulose ???
Xyloglucans ???
8 Hemicellulose ????
?-mannans ?-????
Sources Brillouet Carré, 1983 Bach-Knudsen,
1997 Huisman et al., 1998
27
?????
???
???
???
???
??
??
??
??? ???? ?????? ?????
????? (???,???,... )??????, ???????????????????,??
?????????????
28
??????
????
????
??
???
??
??
????NSP??
?????????
29
Good digestion results in little substrate for
bacteria???????????????
Duodenum???? Jejunum?? Ileum??
Few Bacteria
Bacteria ??
Higher rate of nutrient absorption????
30
???????????????
Duodenum???? Jejunum?? Ileum??
Lower rate of nutrient absorption????
Starch
More Bacteria
Fat
Protein
Lower rate of nutrient absorption????
Bacteria ??
31
???????????????
?? VFA (mmol)
?? VFA (mmol)
??
??? MJ/kg DM
13.78 a
8.3 a
312.3 a
???
10.86 b
118.2 b
329.0 a
?? ?????
14.13 a
5.4 a
930.0 b
?? ????? ?
(Choct and Annison, 1990)
32
?????????
Micro-organism B Eg Trichoderma
Micro-organism A Eg Aspergillus
Micro-organism C Eg E coli
A cocktail of the enzymes A BC??????

• ??????????(2-5 ???)
• ??????(???????)???
• ???????,?????????

33
????????????
??? ??????
??? ???
??????????????? ??????(?????????)
???? ?????
34
????????????(1)
Rovabio ??????
??
Rovabio Excel
????
A.M.E. kcal/kg
?? 54 ?? 36
3091
52
C97-21
?? 44 ?? 15 ??? 7.5 ?????
15
3016
66
C98-06b
?? 54 ?? 17 ?? 10
3197
53
C99-06b
?? 58 ?? 15,8 ???? 8 ??? 9

3072
61
S99-03
?? 54 ?? 36
2968
57
C99-28
35
????????????(2)
??
Rovabio Excel
????
A.M.E. kcal/kg
?? 54 ?? 36
2945
69
C00-61
?? 50 ?? 8 ?? 33
3014
102
C01-07
?? 54 ?? 36
3092
66
C01-109
?? 54 ?? 30
3132
62
C01-133
?? 54 ?? 30
3111
63
C02-05
?? 51 ?? 16 ???? 20
3119
70
C02-05
36
AME ??? ????????
???? kcal/kg
?? kcal/kg
???????????
kcal/kg

???? (n 11)
3.5
3074
66
123
???? (n 8)
3045
85
164
5.3

• RovabioTM 500 g/t
• ??? 65,000 kcal/t 8 kg ?, or USD 4.0
• ??????1.5 8 kg ?? or USD 3.2
• ??16 kg?????

37
????????????7???

1. GMO ? Non-GMO ??
2. ??????????
3. ?????????
4. EU/FDA ????
5. ?????????????????????,??????
6. ????. ???????,???????????
7. Return/cost ????

38
4. ??????????????
DL-??? (DLM, ??)
DL-2-??-4?? (DL-HMB,???)
39
HMB ???? Aspergillus???
40
HMB ???? Fusarium
41
?????(HMB) ???????
Inhibition Zone??? at pH 4.5 (mm, N3) mg/g
O8 O157 O149 ?? ???? ?? HMB, 0 20.3 21.7 20.3 20.
7 30.7 22.74 HMB 1.0 22.0 23.3 24.7 21.7 28.0 23.9
4 HMB 2.0 22.0 22.0 24.0 21.0 27.3 23.26 HMB
4.0 23.3 24.7 30.7 22.3 32.7 26.74 ?????
0.5 21.3 20.7 24.0 20.0 30.7 23.34 ???
0.5 22.7 23.7 24.0 20.3 31.3 24.40  
42
??vs?? ???????

• HMB (DL-2-??-4??) ??????, ??????80-84 ? DLM
  (buying price at 80, using 84)
• DLM ?? 2 kg/t x 2.5 5.0/t
• HMB at 84 2.38 kg x 2.0 4.76/t, save
  0.24/t
• ??????????
• ????????????? USD 0.5-1.0/t
• ?? ???? 50??????? USD 2-3/t
• ?????????
• ??????? 0.34/t, ????? 2.5-5.0/t

43
HMB????????pH????
?? pH
?????, ml HCl

• I II I II
• 1. ?? 6.09 6.09 26.03 27.18
• 2. DLM, 1.0 kg/t 6.05 6.06 24.91 26.79
• 3. HMB, 1.14 kg/t 5.98 5.98 23.70 25.37
• 4. HMB 2.28 kg/t 5.92 5.95 22.80 23.73
• 5. HMB 3.42 kg/t 5.85 5.87 22.75 24.34
• 6. ??? 3.0 kg/t 6.14 6.19 23.71 24.97

?? HMB???? pH?????,????????????7.6-12.5 2.28
kg ?HMB?3.0 kg?????????
44
HMB????????? (56 ????)
.96
.71
1.34
1.13
1.26
.77
?? DLM - ??? 2.69 HMB 1.14 ?????? HMB 2.28
, 6 HMB 3.42
45
HMB ???????

• ???????(USD/t)
• ???????(-12.5) 0.071
• ???? 0.017
• ?? 0.001
• ????? 0.012
• ?? USD/MT ?? 0.101
• ?? USD 30,300

?? 1000 t, 25 d, 12 mo x 0.101
46
5. ?????(????????)

• ??????5-7?,??????????
• ?????????? 37-42?,???????????? 15-20 .
• ???????????????
• Protein, AA, Ca P, vit. min. ????????? 20,
  ??,???????????
• ????USD 10/t ,????????????
• ????? USD 2-3/t ?

47
6. ????????????

• ???????,??????????,???1-1.5???
• ????????,????????
• ???????????? 0.5-1.0
• ??????????1,???? 10,000 mt/mo ????,?????? USD
  300,000 ????

48
????????????

• ???? ????????????,??????,???????, ?? 30????
• ???????,?????????
• ????????,???????
• ????70???,???(1000??/?)??? 15-20 l ?,????1-1.5
  pc???
• ?????? 10-15, ???????? 8-10, ???????
• ?? 2-3 /t?

49
7. ????????

• ?????????,??28?,????12??,?????????
• ??????????,?????????
• ???????
• ??????,???????????
• ????????????,1-3 ml/l
• ?????? VE ? VC ??
• ?????
• ???????????,??????10??
• ??????????5????, 6.5/t?

50
???????? ??????

• ?? ADG, g FCR Live/start
• ?? ???? 76.8 1.93 11/12
• ?? ??? 48.9 2.22 12/12
• ?? ???? 75.0 2.02 9/12
• ?? ??? 45.8 2.42 9/12

???? 22 C. ??? 30-32 C and 22 C, 5
???, ???????????, Knight et al. 1994, Poult. Sci.
73627-639
51
8. ????????????

• ??????
• ????,??????????????
• ???????????? 0.88, 0.72, 0.65, 0.60
• ???? 0.44, 0.36, 0.32
  and 0.30 ?
• ??0.05??,????????????,?????USD 0.50/t?
• ?????
• ?????????????
• ?????????????
• ???? USD 1-2/t ?

52
??

• ?8????????????????
• ????????? ME ? DAA ? (2-3) ?
• ? DAA ???????,????????? ?
• ?????????? ? (2-4/t) ?
• ???????? (2-3/t) ?
• ????????? (1-3/t)?
• ??????????? (2-3/t) ?
• ????????(6.5/t) ?
• ???????? (1/t)?
• ????????, ????????? 10-20/t

53
Thank You !