Sleep Analysis Software SleepSign

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Sleep Analysis SoftwareSleepSign for Animal
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Summary

• 1. History
• Industry standard software developed with
• OBI and accepted world wide
• 2. Data acquisition
• Any devices can be integrated
• 3. Sleep stage scoring
• Easy enough for beginners
• Sophisticated enough for experts
• 4. Post analysis
• Quick and easy reporting

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Osaka Bioscience Institute

• Discovered somnogenic effect of PGD2
• Citation of published scientific papers, world
  NO.1
• KISSEI co-developed SleepSign

Osaka
http//www.obi.or.jp
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International customers

• 13 countries, 240 licenses
• Cited by most highly ranked scientific papers
  

Stanford University Center for Narcolepsy
Harvard Medical School Department of Neurology
Osaka Bioscience Institute Molecular Behavioral
Biology
Eastern Virginia Medical College Department of
Pathology and Anatomy
Tokyo Medical and Dental University Institute of
Biomaterials and Bioengineering
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Korea Institute of Science and Technology Center
for Neuroscience
Beijing university Department of pharmacology
Eli Lilly Pfizer
Asia
India
Fudan university
EU
Jawahar Lal Nehru University
Chinese university of Hong Kong Center for
Neuroscience
Waseda-Olympus Institute. Singapore Center for
Neuroscience
Hôpital Pellegrin. Boudreaux France
GlaxoSmithKline
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Digital data import
BIOPAC
SleepSign
Import directly
DSI Dataquest A.R.T
EDF file
(European Data Format)
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Analogue recording
A/D conversion card
Analogue amplifier
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Analogue recording Video
Camera
MPEG encoder card
A/D conversion card
Analogue amplifier
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EEG is a wave
A wave is defined by Amplitude and Frequency
Amplitude
Frequency
Frequency
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Human sleep EEG
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FFT (Fast Fourier Transformation)
FFT maps the power of each frequency bins in a
wave
Waveform
Power spectra
µV2
Hz
µV2
Hz
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Rat sleep stages
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Wake to NREM
Wake
NREM
NREM
EEG
FFT
High amplitude slow wave
EMG
EMG becomes flat
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NREM to REM
REM
REM
NREM
EEG
FFT
Low amplitude, theta wave
EMG
EMG is flat
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REM to Wake
Wake
REM
Wake
EEG
Not rhythmical
FFT
EMG
EMG becomes active
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Automatic scoring

• Easy operation
• Computer assists criteria decision
• 85-95 agreement to human visual scoring

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Various analysis parameters
Quality of sleep
The number of brief wake
Delta power for sleep stage
Total amount of each stage
Quantity of sleep
Duration of sleep wake episodes
Sleep onset
Evaluate efficacies on sleep regulation
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The number of Brief wake (Hypnogram)
Timescale graph of sleep stages
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Brief Wake between NREM Sleep in Mice
brief wake (lt16 s)
NREM
NREM
EEG
EMG
4 s
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Total amount of sleep stage (Stage Graph)

Total amount spent in each sleep stages per time
bins
NonREM
Wake
REM
REM
Light
Dark
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Mizoguchi Eguchi et al., PNAS 2001
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Duration of sleep episodes (Vigilance State
Parameters)
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Huang, et al., PNAS 2006
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The amount of delta power (Average FFT)
Stage dependent average FFT per time bin
NREM/Light'
NREM/Dark
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Multi Animal ScreeningNew function
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Rat sleep stages
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Simple interface
multi screening is executed after pre-setting
common value of screening parameters
Simple pre setting parameter menu
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Initialize Scoring Ratio
Step1 calc each portions of R/W/NR
W 12
NR 80
R 8
Step2 multiple ratio of Light/Dark phase
NR 80
X
Light phase 80
Dark phase 20
NR 64
NR 16
Dark phase 20
Light phase 80
W 9.6
R 1.6
R6.4
NR 64
W 2.4
NR 16
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Adjusting
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Locmoter
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Summary

• 1. History
• Industry standard software developed with
• OBI and accepted world wide
• 2. Data acquisition
• Any devices can be integrated
• 3. Sleep stage scoring
• Easy enough for beginners
• Sophisticated enough for experts
• 4. Post analysis
• Quick and easy reporting

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Acknowledgments
Dr. T. Mochizuki (Beth Israel Deaconess Medical
Center and Harvard Medical School) Dr. X. Tang
(Eastern Virginia Medical School) Dr. Z. Huang
(Fudan University and Osaka Bioscience
Institute) Dr. O. Hayaishi, Dr. Y. Urade (Osaka
Bioscience Institute) Dr. S. Nishino, N. Fujiki
(Stanford University, Center for Narcolepsy) Dr.
Z. Hu (Third Military Medical University) Dr. S.
Inoue, K. Honda (Tokyo Medical and Dental
University) Dr. N. Eguchi (Waseda-Olympus
Bioscience Reserach Institute)