Professor Ramaz Gakhokidze

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Application of New Ecologically Pure Bioorganic
Methods in the Agriculture
Professor Ramaz Gakhokidze Head of Department of
Bioorganic Chemistry of Tbilisi State
University, Georgia Academician of International
Engineering Academy State prize Winner E-mail
rgakhokidze_at_gmail.com
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• Providing by food the growing population of our
  planet demands to carry out the following
  measures
•  
• 1. Constant perfection of agricultural system
  management and technological processes.
• 2. Intensification of soil cultivation by
  application of effective, complex factors.
• 3. Reproduction growth and rational usage of
  soil fertility.
• 4. Revelation of maximal potential facilities of
  the plants by using of bioorganic methods.
• Nowadays the scientists are trying to solve the
  problem of world food supplies by help of the
  first three items.
• We consider the fourth item suggested by us as an
  efficient way of productivity increase and
  augmentation of qualitative characteristics of
  agricultural produce.

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• A basic reason of ecological crisis is an
  imperfection of technology of agricultural
  production intensification. Agricultural
  production today is based on the application of
  fertilizers and pesticides which is one of the
  basic reason of environment pollution.
  Environment pollution causes a damage of genetic
  apparatus, to which are connected not only
  hereditary diseases, inborn deformities,
  malignant tumors, but such illnesses, as
  cardiovascular, neural, digestive system, immune
  and other ones. With the increase of pesticides
  application there were risen a number of mentally
  retarded children. By data of USA ministry of
  health explains the population death-rate
  increase, hypertension, cancer and cirrhosis by
  environment pollution with pesticides and other
  harmful substances. This is the greatest danger
  which threatens to mankind.
• Unfortunately, today it is impossible to refuse
  the chemicalization in agriculture. The use of
  chemicals in agriculture nowadays does not
  guarantee the high quality and safety of the
  product. Foodstuff safety is duty of state.
  Improvement of existed situation from position of
  environment protection is possible by reduction
  till minimum of negative influence of
  chemicalization of agriculture.

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• One of the direction of solving of this problem
  is the application of bioregulators. Tens of
  billion of dollars are spent by the firms on
  creating of new regulators. In spite of this,
  these preparations are far from being perfect.
• On the basis of a new scientific discipline -
  agrobioorganic chemistry, on the basis of natural
  products we created non-toxic bioregulators of a
  new type bioenergyactivators, which have no
  analogies. They enable to receive ecologically
  pure, rich harvest of high quality and increase
  immunity through the augment of vital potential
  in plants by minimal chemicalization and to use
  land to the maximum and efficiently, that is of
  special importance for countries having
  insufficient arable land. Nowadays, there is no
  remedy in the world which can increase
  productivity of agricultural crops and at the
  same time simultaneously grow green mass and
  qualitative indices.

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• Bioenergyactivators are regulators of a new
  generation, which principally differ from
  well-known chemical remedies and enable to manage
  endogenous (self) regulatory systems of plants.
  They have been used for cultivation of cereals,
  melons and vegetables. The crop capacity has been
  increased significantly yielding ecologically
  pure products. At the same time, composition of
  protein, limited amino acids, vitamins,
  microelements and other bioactive compounds
  grows in fruit. For instance, vitamin C in maize
  increases by 50 , lysine by 80 , iodine by 960
  , selenium by 200 .
• Bioenergyactivators have been found to facilitate
  the rooting of grafts of toughly rooted plants,
  which demonstrated good results on silver spruce
  (picea pungens Engelman), kiwi, vine, nut,
  kazanlyk rose, geranium, gladiolus and other
  valuable plants. The obtained results
  significantly exceed all the known data obtained
  by other methods in this area.

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• Silver spruce is a valuable plant both from
  decorative and ecological point of view. It
  releases a large amount of phytoncides (which
  clear the air from microbes) and, in its turn,
  absorbs heavy metals, pesticides and other
  poisons available in the environment, i.e. it is
  a natural filter. It is difficult for silver
  spruce to strike root, therefore it is germinated
  by seeds which can yield fully-developed plant
  only within 10-15 years and with a small outcome.
  The use of bioenergyactivators allows for this
  unique wood genus to strike root by 90-85 and
  significantly accelerates its growth and
  development.

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• Rooting of valuable decorative plant silver
  spruce.
• On the left nontreated grafts.
• On the right treated grafts.

Silver spurce seedlings obtained by use of new
technologies.
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3 Year silver spruce rooted by use of new
technologies. The plant reached a height of 2
metres and 20 cm at the age of 7 years.
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• Using the new method it would be also possible
  for the tillers of actinidia-kiwi to strike root.
  Kiwi or yangtao actinidia is a subtropical berry
  culture. It is one of the richest plants in
  biologically active substances. It has from
  ancient time been used both as food, so for
  treatment. In China it has long been used to
  prevent quite a number of diseases, including
  cancer. As demonstrated by our research, the use
  of bioenergyactivators results in a substantial
  increase in the plants content of bioactive
  substances enhancing strongly its pharmacological
  and therapeutic activity.

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Effect of bioenergoactivators on rootage of
kiwi graft (after 60 days). On the right
nontreated (control version). On the left
treated.
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• Vine grafting has a long-term history. In spite
  of this, the yield of the first quality grafted
  saplings is rather low. Bioenergyactivators have
  a high effect on the yield of saplings. They
  promote rooting and provide active performance of
  current vital processes resulting in their
  full-fruitage development within a short period
  of time.
• At present, considerable attention has been
  focused upon artificial immunization of plants
  which can be considered a powerful means in
  increasing nonspecific resistance against
  diseases and various unfavourable influences.
• This innovation is a strong remedy to increase
  stability of plants towards diseases and other
  unfavorable conditions. Some compounds acquired
  in our laboratory show strong immunologic effect
  on the lemon sorts growing in Georgia, against
  Mul secco diseases, caused by fungus Phoma
  tracheifila, which causes drying of lemons,
  oranges, and the rest of citruses and damages
  crop of citruces. It should be noted that the use
  of new biopreparations sharply increases frost
  resistance and promotes fruiting by several
  years.

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Influence of the new preparation on lemon (New
Georgian variety) resistance On the left
Mal secco indusing fungi (Phoma tracheifila)
inoculated plant (nontreated). On the right
Mal secco indusing fungi (Phoma tracheifila)
inoculated plant (treated).
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• New preparations strengthen the plants resistance
  against outside factors and diseases, which
  provides the means of getting ecologically pure
  agricultural crop without polluting ground by
  poison. They penetrate into the plant cell, play
  a role of inductors of general unspecific
  durability, and stimulate protection function of
  the plant by activation of reserved mechanism.
• Plant mycoplasmic diseases are wide spread and
  cause considerable damage to agriculture.
  Mycoplasmic as well as virus diseases, because of
  specificity of pathogenic microorganisms and the
  widespread of infection, belong to plant diseases
  with a practically impossible cure. As it is
  known, recently, leaf rugosity a mycoplasmic
  disease of the mulberry-tree had a serious
  negative influence of the nutrient source of
  sericulture. For restoration of the sericulture
  it is very important to restore the nutrient
  source, namely to conduct some activities
  against the leaf rugosity diseases.

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• We have demonstrated that the treatment of
  mulberry-tree by energyactivators the resistance
  of the plant to the mycoplasmic disease
  increases. The processed plant could recover its
  immune system on account of utilization of
  reserve substances. Growth and development of
  the plant is stimulated, active elements of their
  anatomic structure and of productivity indices
  are improved, and spreading of the mycoplasmic
  disease is decreased all of these can be
  considered as increasing of general resistance of
  the plants to the disease, as well as to
  unfavorable environment factors.

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As it is seen from Table 1, the number of
diseased plants was decreased by 70, and
progression of the disease decreased by 60.4,
in comparision with the control data.
Table 1 The Effect of Bioenergyactivator on
Resistance of Mulberry Tree to the Leaf
Rugosity Disease (an old plantation)
Variant Number of experimental plants Number of infected plants Percentage of infected plants Disease development,
Control Test 20 20 18 4 90.0 20.0 67.5 7.1
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leaf rugosity of mulberry tree (nontreated).
The dried tree because of leaf rugosity
revived within 2 months after pouring of
bioenergoactivator (2L) into its roots and even
gave fruit (no disease is noted).
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• Germination energy and sprouting capacity of the
  mulberry seed are quite high in comparision to
  the control variant (Table 2).
• Table 2
• The Effect of Bioenergyactivator on Germination
  Energy and Sprouting Capacity of the Mulberry
  Seed

Variant Soaking method Soaking method Spraying method Spraying method
Variant Germnation energy, Sprouting capacity, Germination energy, Sprouting capacity,
Control Test 81.3 96.6 89.3 98.0 80.6 86.6 89.3 94.0
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Roots of saplings grown from mulberry tree
seeds (4 months later after sowing) On the left-
control version. On the right- treated.
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• Proteins carry out a huge role in the ability for
  plants to live. A feed of silkworm with highly
  nourishing leaves increases the average weight of
  cocoons and quantity of an unwinding string.We
  determine influence of bioenergyactivator on
  protein content in leaves of mulberry (in spring
  and autumn) and in haemolymph of caterpillars of
  silkworm. Quantity of proteins both in leaves of
  a mulberry and in haemolymph increases
  considerably because of influence of
  bioenergy-activator. In particular, in
  experimental spring leaves the quantity of
  proteins increases by 3.6 more than in control
  variant, and in autumnal leaves - by 88.5
  (Table 3).
• Table 3
• Influence of Bioenergyactivator on Protein
  Content of Total, Non-Protein and Protein
  Nitrogen in Control and Experimental Leaves of
  Mulberry

Variant In spring leaves In spring leaves In spring leaves In spring leaves
Variant Total nitrogen, Non-protein Nitrogen, Protein Nitrogen, Quantity of proteins,
Control 3,09 0,48 2,61 16,3
Test 3.26 0.56 2.71 16.9
In autumnal leaves In autumnal leaves In autumnal leaves In autumnal leaves In autumnal leaves
Control 1.68 0.3 1.38 8.62
Test 2.9 0.3 2.6 16.25
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• Quantity of proteins in haemolymph of fifth age
  of test variant for 3rd day increases by 10.4,
  and for 8th day by 21.5, to surpass a control
  variant (Table 4).
• 
  
  
• Table 4
• Influence of Bioenergyactivator on Quantity of
  Proteins in Haemolymph

In haemolymph of fifth age Day Quantity of proteins Quantity of proteins
In haemolymph of fifth age Day Control Test
In haemolymph of fifth age III 9.48 10.47
In haemolymph of fifth age VIII 10.21 12.41
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• As a result of influence of bioenergyactivator
  the quantity of proteins in leaves of a mulberry
  and in haemolymph caterpillars of silkworm, we
  see significant changes in biotechnological
  indices. Application of the bioenergyactivator
  increases the total weight of silk, improves the
  type of silk, the thread and the average length
  of the thread (Table 5).
• Table 5
• The Effect of Bioenergyactivator on
  Technological Indices of the Cocoon

variant Total weight of living cocoon, g Kind of sericulture, Outcome of thread, Aaverage length of thread, m
Control Test 2.30 3.70 41.07 45.79 36.61 43.75 1390 1580
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• Bioenergyactivators are ecologically pure and
  safe. They provide natural normalization of
  plants. They enable preservation of homeostasis
  through activation of physiological regulatory
  mechanisms, providing adaptation to the extremal
  conditions. They influence on the various system
  of metabolism breath, photosynthesis,
  biosynthesis of proteins, carbo-hydrates and
  secondary substances. They cause sharp increase
  an activity of some enzymes, for example
  katalases, peroxidases, change physical and
  chemical properties of protoplasm and
  penetrability of cellular membranes for water,
  mineral and organic substances.
  Bioenergyactivator stimulates the nitrate
  assimilation and protein levels in the bean
  primary leaves by using nitrate as single
  nitrogen source at the early stages of
  vegetation.
• There are two ways of adaptation of human with
  environment. The first improvement of
  environmental preservation, the second increase
  the stability of organisms towards the action of
  harmful factors.

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• Adaptation of living organisms to the different
  conditions is possible through the usage of
  bioenergyactivators. They reveal strong
  antioxi-dant properties which give the means of
  reduction of negative influence on human being
  caused by chemicalization of agriculture.
• In the laboratory mice antimutagenic and
  anticytotoxic effect of bioenergyactivators
  (biorag, ragozan, ematon and ragil) have been
  studied during mutation and cytotoxicity induced
  with the fertilizers and the pesticides.
  Experiments showed that tested bioenergy-activator
  s exerted highly effective antimutagenic and
  anticytotoxic action.
• By obtained results it is possible to increase
  the quantity as well as quality of agricultural
  products, and at the same time, to reduce
  negative influence of agricultural
  chemicalization on the environment, which will
  give great economic, ecologic and social effects.
  

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Examples of using new biotechnologies
On the right- sunflower obtained by use of new
technologies (seed oiliness increased 1,5
times ). On the left- control version.
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examples of using new biotechnologies
Vegetable marrow On the left nontreated.
On the right treated.
1,5 Year apple tree treated by
bioenergoactivator.
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examples of using new biotechnologies
Herbal plant (kalanhoe) 8 months after sowing.
On the left nontreated.
On the right treated.
Sugar beet On the left nontreated.
On the right treated.
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examples of using new biotechnologies
Effect of new technologies on cucumber and
dwarfish pomegranate.
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examples of using new biotechnologies


On the left- control
version (unripe).
On the right- treated (picked 5 times).
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examples of using new biotechnologies
Mexican cucumber (chaiot) obtained by use of new
technologies .
Potato obtained on the marshy soil by use of new
technologies. On the left- control version. On
the right- treated.
On the left control version. On the
right treated.
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examples of using new biotechnologies
Exsperiments in the conservatory of Tbilisi
State University
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examples of using new biotechnologies
One plant of the maize sort Kartuli krugi
usually gives 1 corn-cob only 1-6
characterized by 2 corn-cobs. The use of new
biopreparation 70 of plants give 3 corn-cobs,
and 30 from 4 to 7 corn-cobs.
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examples of using new biotechnologies
Maize crop yielded with the use
of new biopreparation.
Effect of bioenergoactivators on the maize roots
system.
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examples of using new biotechnologies
Chickens of the same age. On the right- control
version. On the left- chicken fed with new
biopreparation during 20 days.
Non-castrated,10 months old pig (species
landras) grown to 320 kg by use of
biopreparation.
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Results achieved by use of new biotechnologies in
farm household
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Results achieved by use of new biotechnologies in
farm household
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Results achieved by use of new biotechnologies in
farm household
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Results achieved by use of new biotechnologies in
farm household
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Improvement of chemical composition of
agricultural crops by use of new technologies
Agriculture crops Indices Type of treatment Type of treatment
Agriculture crops Indices Untreated sample Treated sample
Maize Protein, 9,20 10,76
Maize Fat, 3,56 4,75
Maize Lyzine, g/kg 3,24 6,42
Maize Tyrosine, g/kg 1,32 3,21
Maize Iodine, mg/kg 0,06 0,58
Maize Selinium, mg/kg 0,36 0,74
Sugar-beet Sucrose, 10,3 22,5
Wheat (sort Dolis Puri) Wet Gluten, 25,6 43,2
Grape (sort Izabella) Sugar content, 12 24

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The agricultural products obtained by the use of
bioenergyactivators due to their ecological
purity and high nutrient content (protein,
limited amino acids, vitamins, microelements)
are valuable for babies nourishment. Based on
current research of physicians and hygienists
the crop obtained with bionergyactivators is a
good means for preventing goitre, cardiovascular
and other diseases.
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• NEW BIOTECHNOLOGIES ENABLE
• to receive biologically pure harvest non
  polluting environment
• to significantly increase crop capacity
• to speed up development of plants (to abridge
  ripening terms, to receive early harvest)
• to improve quality of harvest (increase content
  of microelements, protein, carbohydrates, limited
  aminoacids, vitamins and other bioorganic
  substances)
• to increase green mass
• to intensify stability towards diseases and
  unfavorable conditions of environment
• to increase effectiveness of fertilizers, which
  enables economy of mineral fertilizers