Laboratory Filtration

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Laboratory Filtration
Cassidy Markee Director of Laboratory Division
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Spectrums Core Technology Membrane Separation

• Spectrum offers products for two kinds of
  Membrane Separation (membrane, systems,
  components and accessories)
• Membrane Dialysis passive diffusion through a
  semi-permeable membrane 100 to 1,000,000
  Daltons
• Membrane Filtration applied pressure across a
  selective membrane. 3 types based on size range
• Macrofiltration 5 µm and up
• (Woven Mesh Screens)
• Microfiltration 0.05 5 µm
• (Direct Flow and Tangential Flow)
• Ultrafiltration (Molecular filtration) 5 kD to
  0.05 µm
• (Tangential Flow only)

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Membrane Microfiltration in the Lab

• Two membrane configurations for Direct Flow
  Filtration (DFF)
• Flat sheet DFF Devices - disadvantages
• Less surface area - lower flux rate
• Quicker to plug or foul
• Lower particle load
• Loped Hollow Fiber DFF Devices - advantages
• Higher surface area - higher flux rate
• Longer filter life less fouling
• High particle load

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Mode of Action Flatsheet DFF
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Mode of Action Hollow Fiber DFF
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Spectrums Hollow Fiber DFF Devices

• Features Benefits
• Sterilizing grade 0.2 um Mixed Cellulose Ester
  (ME) - DynaFibre
• Looped hollow fiber membrane provides large
  surface area
• Higher flux rates and increase filtration volume
• Filtration from outside fiber into lumen reduces
  fouling and increases particle load
• Compact and narrow filter housing
• PP air vent fiber loops prevents air lock

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Hollow Fiber Microfiltration 4 Types of Filters
MediaKap MediaKap Plus Cold sterilization of
culture medium
CultureGard Sterile barrier for feeding/perfusio
n of bioreactors
MiniKap Point-of-use water filtration
DynaGard Syringe tip filtration
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DynaGard Syringe Tip Filters

• Aqueous solution filters (blue)
• 0.2 µm Cellulose Ester Membrane
• 3 sizes for 1-20 ml
• Organic solution filters (white)
• 0.2 0.45 µm Polypropylene Membrane
• 2 sizes for 1-20 ml
• Low fouling
• Irradiated or non-irradiated
• Narrow housing design
• Luer-lok connections

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MediaKap MediaKap Plus HF Filters

• MediaKap for serum-free media
• MediaKap Plus for serum-enriched media
• Skinned DynaFibre for higher viscosity
• 2x filtration capacity for serum-free media
• 0.2 um sterilizing grade cellulose ester
  membrane
• 5 sizes 2, 5, 10, 25 50 liters
• 0.2L 100L (400ml/min 2L/min)
• Low extractable
• Optional filling bell protects sterility
• PP vent fiber prevents air lock

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CultureGard HF Perfusion Filters

• Sterile protection during culture perfusion,
  feeding or sampling
• Large surface area
• Minimal hold-up volume
• PP vent fiber prevents air lock
• Process highly viscous solutions
• Use two in series to increase duration of
  feeding

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MiniKap Water and Gas Filters

• Point-of-Use aqueous final filter
• Removes particles in air/gas lines as low as
  0.01 µm
• Validated for use in clean rooms
• Industries served
• Electronics/Semiconductors
• Medical Device Manufacturing
• Biotech/BioPharm
• Petrochemical

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MacrofiltrationSpectra/Mesh Woven Filters

• 5 Material Types
• Nylon
• Polypropylene
• Polyester
• PEEK
• Stainless Steel

• Applications
• Cell Tissue
• Industrial
• Environmental
• Air Particulates
• Pharmaceutical
• Research
• Food Beverage
• Waste

Mesh Opening 5 1000 micron Sizes 25, 47, 55,
90 150mm discs 30x30 cm sheets
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Reference size range and scale
Macrofiltration Microfiltration Ultrafiltr
ation Dialysis
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Thank You??