ELISA

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ELISA
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• Serological test
• Antibodies are specific for the antigen
  stimulated their production
• Indirect ELISA
• Patient serum ahs antibodies again a certain
  antigen
• Patient has been expose to that pathogen

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Antibodies against HIV Secondary
antibodies Against human Antibodies.
Neisseria gonorrhoeae
Enzyme linked antibodies
Substrate colorless Product blue
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• 6 patients
• History suggests they might have picked up HIV.
• Infects CD4
• Virus sometimes becomes latent
• No symptoms
• Eventually virus destroys T cells
• Susceptible to opportunistic infections

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• Transmitted through
• Blood
• Other body fluids (sexual contact)
• Contaminated needle

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1/100 1/102 1/10,000
1/104 1/1000000 1/106
10,000 100,000 1,000,000
10,000000
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• microbes in 1g soil
• colonies X reciprocal of the dilution
• 50 colonies
• Dilution 1/10,000
• (50) (10,000) 500,000 cells/gram of soil