FAMILY:

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• FAMILY
• VIBRIONACEAE
• Prof. Khalifa Sifaw Ghenghesh

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• 3 Important Genera
• Vibrio
• Aeromonas,
• Plesiomonas
• Oxidase ve
• Habitat

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??? ???? ?????? ??????Vibrio cholerae

• I. V. cholera O1
• Agglutinate in Antisera Directed Against O1
  Antigen.
• Produce Enterotoxin.
• 2. Non-Toxigenic V. cholera O1
• 3. Non O1 V. cholera (NCV, NAV)
• II. V. cholerae O139

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Vibrio choleraeGram-negative, curved rods
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Vibrio cholerae Leifson flagella stain
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Vibrio choleraecurved rods with polar flagellum
(EM)
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• V. cholerae O1
• --------------------------------------------------
  -
• Biotye
• El-tor Classic
• Haemolysis (SRBC) --
• VP --
• Resistance to PB --
• --------------------------------------------------
  -

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• VIRULENCE FACTORS
• Enterotoxin CT
• Adherence Ability to Attach to the Microvilli at
  the Brush Borders of the Epithelial Cells.
• EPIDEMIOLOGY
• Causes Widespread Disease.
• Human Carriers
• Modern International Travel

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• PATHOGENESIS CLINICAL INFECTION
• Infectious Dose
• Incubation period
• Abrupt Onset of Diarrhoea Vomiting
• Stool gt Watery (Rice Water), Odorless
• Fatality Rate

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• LAB. DIAGNOSIS
• Isolation Identification of the Organism.
• Media TCBS
• Transport Media
• TREATMENT
• Prompt Replacement of Fluid Elecrolytes
• Tetracycline and others
• PREVENTION CONTROL
• Adequate Sewage Treatment and Water Purification
  Systems.
• Vaccination ??

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Typical Vibrio cholera contaminated water supply
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Vibrio parahaemolyticus

• Halophilic vibrio
• Associated with seafood
• Gastroenteritis
• Outbreaks of Food Poisoning
• Extraintestinal infections gt wounds
• Virulence Factors
• Haemolysin gt
• heat-stable cytotoxin
• Heat-labile enterotoxin
• Adherence to human intestinal cells

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Aeromonas species

• A. hydrophila, A. sobria A. caviae
• Enterotoxins, Cytotoxins, Haemolysins, Etc
• Gastroenteritis, Septicaemia, and Skin Wound
  Infections
• TREATMENT
• TMP-SMX, Tetracycline, Gentamicin
• Resistant to b-Lactam Antibiotics.