CELL BIOLOGY COURSE

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????? ??
??8345002 ??trg_at_cella.cn ??http//www.cella.cn
CELL BIOLOGY COURSE
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??? ?????

• HISTORY AND PERSPECTIVE

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??????

• ??? ?????
• ???????
• ??????
• ??????????
• ?????????
• ??? ?????????
• ??? ??????
• ?? ????????

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??? ?????

• ?????????????????????????
• 1)????,????????????
• 2)????,????????????
• 3)????,?????????,????????????

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• ?????????(Cytology)?????
• ?????????????????,??????, SCI?????????????????4.
  1?
• ????????????????????

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• ????????????????
• 1)1665-1830s,????,??????
• 2)1830s-1930s,????,Cytology???
• 3)1930s-1970s,??????, Cytology?????????
• 4)1970s??,??????????

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???????

• ?????????????????????
• 1590?J. ?Z. Janssen???????????
• 1610?Galileo Galilei?????????
• 1665????Robert Hooke????????????,????cells?????
  ?

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• Robert Hooke and his cells

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Robert Hookes microscope
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Micrographia
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Micrographia
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• 1680?A. van Leeuwenhoek???????????????????????????
  ??????????????????????????????????????
• Hooke???160???,???????????????1830s????????,??????
  ?????????????

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Made by A.van Leeuwenhoek (1632-1723). Magnificati
on ranges at 50-275x.
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• 1831?R. Brown?????????????????
• 1836?GG. Valentin??????????????????
• ????????????????????

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??????

• Cell Theory?19?????????,????????
• ???????????
• ??????????????
• ????????????????

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• ???????(MJ. Schleiden)???(T. Schwann)??????????
• ?????19?????????????????BC. Dumortier?JB. de
  Lamark?CB. Milbel?H. Dutrochet?R. Brown?JE.
  Purkyne?R. Remak?R.Virchow?????????

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Jean-Baptiste de Lamark
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• 1838?Schleiden??????? ,?????????????????????free
  -cell formation??????????
• Schwann???????(Cell Theory) 1839??????????????
  ?????????

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Theodar Schwann
Matthias Jacob Schleiden
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• Schwann??
• ??????????
• ??????????????
• ???????Schleiden???????
• 1855 ???R. Virchow ??omnis cellula e
  cellula????????????????

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Rudolf Virchow
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• 19??30???????????????????????
• F. Dujardin(1835)????sarcode
• JE. Purkinje(1839)?von Mohl(1846)????????protopla
  sm ?
• ??????????????????,??????????????

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(No Transcript)
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??????????

• 1932????E. Ruska?M. Knoll??????,?????????? ?
• 1939?Siemens?????????
• 1940-50s??????????????????????????????????????????
  ????Cytology???Cell Biology?

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???????????

• 1869????F. Miescher ??????????,???????
• 1944?O. Avery?????????,1952?M. Chase??????????????
  ??????????

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• ???????

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• 1952?Franklin??????DNA??X-?????1953????DNA???????,
  ??????

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• 1953?,Watson ? Crick??DNA???????Wilkins??1962
  ??????????? ?

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• 1958 ?Crick ????????????
• 1961-1964?Nirenberg ????????
• 1972?DA. Jackson,RH. Symons?P. Berg??DNA?????
• 1973?SN. Cohen?HW. Boyer???????????,??????????
• ???????????,?????????????????????

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• 1983?,Mullis??PCR?,?1993?????????

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• 1990?,???????????????? (Human Genome Project)?
• ???1993??????,????1???,???3????????30Mb??????
• 2000?6?28?????????????
• ??,???????????????????????????4???????????????????
  ????(ADA)???????

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1996?7?5?,???????????????????????????????
Dolly with her first born lamb
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EMBRYO COLONING
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• 2001?,???Leland Hartwell????Paul
  Nurse?Timothy Hunt????????????????????????

Leland H. Hartwell
R. Timothy (Tim) Hunt
Sir Paul M. Nurse
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2002?,??????????????????????,?????????????????????
??????????????????

• John E. Sulston

• Sydney Brenner

H. Robert Horvitz
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2003?,??????????????????,??????????,????????????
????????
Peter Agre
Roderick MacKinnon
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• 2004?,???Richard Axel?Linda B. Buck??????????,????
  ?????????????

Richard Axel
Linda B. Buck
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• 2005?Barry J. Marshall ?J. Robin Warren
  ??????????,????????????????????????

Barry J. Marshall
J. Robin Warren
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• 2006????Andrew Z. Fire?Craig C.
  Mello??RNA?????????????????

Craig C. Mello
Andrew Z. Fire
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• 2007???????????????????????????????????????????
  ??????

Oliver Smithies
Mario R. Capecchi
Sir Martin J. Evans
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??? ?????????
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???????????????

• ???????????
• ?????????????????,????????????????????????????????
  ????
• ????????????????

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?????????????????????

• ?????????????
• ???(vitalism)
• ???????(reductionism),?????
• ???(holism),??????
• ????????????,???????????????DNA???????????????????
  ???

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???????????

• ?????????????????????????????
• 1.?????
• 2.?????
• 3.?????
• 4.?????
• 5.?????

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????????????

• ??????,???????
• ??????????,????????????????????????,???????????
• ??????????????????

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???????????????

• ???????,???????,??????????,??????????????????
  ????????????????????????????
• ??????,???????????
• ???????? ?????????????

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??????,??918????????????JCB?PNAS?JBC?
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???????????,??1955???????
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???????????,??1905???????
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???????,??1915??????,?????????????????
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Pubmed,??????????,???????????,?????????????
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• ?????????,???????????
• ???????????(????)
• ???????
• ??????????
• ??????????(NSTL)
• EBSCOhost???????
• Elsevier(???,??????)
• Springer??????

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??? ??????

• ?????????????,18??60??????,20??50?????????????????
  ?????7580?,????20??????????????,???????????????
  ??

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????????,?????????

• ?????????????
• ??????15??????,????????2010?????40?
• ???????????????????????????????????
• ?????????????,2004 ?????????????????8100????

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Gene Chip
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????????,??????

• 20???????????????????????????20????40????????70???
  ????????????????????????????????????
• 21????????????????,???????????????????????????????
  ??????,???????

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????????,???????

• 20??60????,???????????,??????????
• ????????????????????????????????????????????,?????
  ???????????????????,??????????

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???????,??????

• ??????????????????,????????10???????????,?5???????
  ????????
• ?????????????????????????,???????????????,????????
  ????????,?????????

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????????,??????

• ????????????????,??????????????
• ??????????18000??,???640??????????????????????????
  ,????????????????
• ????????????????,??????????????????????????

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?????????????

• ???????????
• ??????????????????
• ??????????????????
• ????????????
• ??????????????

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??????????

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• ??????????

• ??????? ??? ????? 2001?03?
• ?????????? ??? ????????? 1999?01?
• ????? ??? ????????? 1998???
• ????? ????? ??????? 2000?8???
• ?????(???) ??? ?????????1998?11???
• ????????????(???) ??? ????????????1997?05???
• ???????(???) ??? ???????1997?07???
• Molecular Biology of the Cell 4th Edition, Bruce
  Alberts et al., 2002
• Molecular Cell Biology 4th Edition, Harvey Lodish
  et al., 1999
• Cell and Molecular Biology 3rd Gerald Karp, 2002