From: www.kinetekpharm.com

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Understanding the complex networks within a
cell...
Parameters 10,000 genes gt100,000 proteins
Approach measure model predict
From www.kinetekpharm.com
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Understanding the complex networks within a
cell...
Parameters 10,000 genes gt100,000 proteins
Approach measure model predict
From www.kinetekpharm.com
Measure quantify amounts of parameters versus
time
Nearly possible
Major challenge
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Understanding the complex networks of a cell...
Parameters 10,000 genes gt100,000 proteins
Approach measure model predict
From www.kinetekpharm.com
Measure quantify amounts of parameters versus
time
Nearly possible
Major challenge
An instantaneous readout of these parameters will
accelerate fundamental advances in biology and
enable

1. Drug discovery (cellular responses to drugs and
   environment)
2. Medical diagnostics (tumor identification and
   treatment)
3. Biowarfare detection (rapid pathogen
   identification)

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Wheres the bottleneck?
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Todays technology
Genomics
104 genes
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Todays technology
Proteomics
Genomics
10 proteins
104 genes
Affymetrix
Sorger Lab, MIT
concentration
time (min)
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Todays technology
Proteomics
Genomics
10 proteins
104 genes
Affymetrix
Sorger Lab, MIT
concentration
time (min)
Measurement time few hours to a day!
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Electronic detection of DNA hybridization
DNA hybridization
Glass
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Electronic detection of DNA hybridization
DNA hybridization
Glass
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Electronic detection of DNA hybridization
Silicon
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Electronic detection of DNA hybridization
Silicon
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Electronic detection of DNA hybridization
Silicon
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Teaching 6.151 Microfabrication Project
Laboratory
Class of Spring 2002
Goal Integrate microfluidics with
microelectronics
Instructors Martin Schmidt and Scott Manalis