Supplementary Table 1

1
Supplementary Table 1
2
Supplementary Table 2
3
Supplementary Table 3
4
Supplementary Table 4
5
Supplementary Table 5
6
Supplementary Figure 1
A
B
80S
80S
Absorbance at 254 nm
Absorbance at 254 nm
polysomes
60S
60S
40S
40S
10-50 sucrose
10-50 sucrose
0.80
0.45
0.70
0.40
0.35
0.60
0.30
0.50
Relative mRNA level
Relative mRNA level
0.25
0.40
0.20
0.30
0.15
0.20
0.10
0.10
0.05
0
0
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
Fraction
Fraction
7
Supplementary Figure 2
TCTAACATCATGTCCTCCCTCCCACTTGCCAGGGAACTTTTTTTTGATGG
GCTCCTTTATTTTTTTCTACTCTTTTCAGGCGCACTCTTGATAAATGGTT
AATTCAGAATAAAGGTGACTATGGATATAATTGAGCCCTCTGGTCCAGGT
CTCAGTTTACCTAATATTACCTCAGAAAGGATATGGAGGGAAGATGATCT
TTTTGCCAGGTCTGACTTTTCTTCCTGCTCCGCCCTCCATTAACGCTCAG
TACCCTTTAGCAGCTGACGGCCCCACGTTCTACTCCATGCTTGGCTTCCT
TTCCAACTAGCTCTTTCATATATTTTACTTGCTAGTATCTCCATTCTCTC
TAAAGTAGTGGTTCTTTTTGCCCTTAAACTTAAATTTTTAAATTAATTAA
CCTGAATTAATAATACATGCACTTAATGTAACATGCAAACAGTACAAAAA
CATGTAGTGAAAAATATTTCTTCCAGAGCTGGGTGTGGTGGCTCATACCT
GTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAAGA
GAGCGAGACCATCCTGACCAACATGGTAAAACCCCGTCTCTACTAAAAAT
ACAAAAATTAGCTGGGCGTGGTGGCACGCACCCCTAGTCCCAGCTACTGG
GGAGGCTGAGACAGGAGAATCGGTCGAACCCGGGAGGCAGAGGTTGCAGT
GAGCCTAGATCGCGCCACTGTACTCCAGCCTGGCAACAGAGTGAGACTCC
GTCTCAAAAAAAGAAGGAAAAATGTTTCTTCCATCCCTATAATCCAGTCA
TCTGCTTCCTGCTTCCCTTCCTGGAGGAAACTTCAGCTACTAATTTCTTA
TGTTTTTTAAGAGATATTCTGTTACTGTGTAAAGTATACATACATACAGA
CACATGCCCCCTTTAAATTTTTTAGATTTATTTATTTATTTAGAGACAGG
GTCTCACTCTAGCCCAGGCTGGAGTGCTGTGGCGTAATCTTGGCTCACTG
CAACCTCCGCCTCCCGGGCCCAAGTGATCCTCCCATCTCAGCCTCCTGAG
TAGCTAGGATTACAGGCGCACACCACCAATGCCCAGCTAGTTTTTGTGTT
TTTCATAGAGACAGGGTCTCACCATGTCATTCAGGTTGGTCTTGAACTCC
TGGGCTCAAGCAGTCTGCCTGCCTTGGCTTCCCAGTGCTGGGATTACAGG
CGTGAGCCACCGTGCCCGGCTAAAAAGTATTTTTAAGTTCTGCATATTGC
TTATTTCACTTAACACTATATTAGAGATTGTTTTATATCAATACATATAG
ATATGCTTATTCTTGTTGACAGTTGCATAATTTTCCATTAAATTGATGTA
TCATGGGCAGTTAACCAGTTACTCGTTTTACTCTTAGCATAACTTTAGGG
AACAATGTGGATGTTTTGTGGTTAAAGCTATTAAAACAGTGGTTCTTGAC
C
8
Supplementary Figure 3
A
PSMB2 3 UTR
1st ALU RC
2nd ALU RC
WT
Firefly
Renilla
B
PSMB2 3 UTR
1st ALU RC
2nd ALU RC
WT
Firefly
Renilla
9
Supplementary Figure 4
CCTTGCCTTTAAAGGCCACTGCATGAGGTGGGGAAGGAGATTTGACTTCC
ACTTGCCTCGGTGACTTTGTCTGTTAAGCAGAACCATAATACTGGCCCTT
CCCCTCCTGTGCTTTGGGCAGTGACTCTCCCTAGCTCATTGGGAACCCTG
GTTGCCCAAACAGCTCTCAATCCAACACTAGAGGTGTGGGGGGTCACTGT
GGAATGTTGGGGGGCAGGCCCCAGTATGAGAGAAAAACCCAGAGGGATGG
TCCTGATCAGGGCTACCCATGCCCCTGAGGATGGTGGAATGAAGGCAGAG
TCGGGGGCTGCATAAAGGAAAGTCTCTTCCAGGGACCAAAGCTTGGAGAA
CTGAGGGTGGAAGGGGGATTAAACAGAAGTGGGTAGGGCAGAGCCTTCTG
CCTGTGGGCTGGATTTCCTCAGGCAAGAAGCCTGTGGGTAAGCTCTGTAT
AATGCGAGGAGGAGTCCCCACAAAGCAGGCTGGTCAGTGGTCACGCTAGT
GTGAGTCTTTCAAACATCCCGTGTAAGTAGATACTGTCATTGCACAGAGG
AGGAAATGGAACCTGGCCCAAGGTCTCAAGAAACAACCCAGCCGGGATTC
AGACTCGGCAGCCTGCGCACTTAACAGCCGTGAGCAACCAGGAACTCCCC
AGCCCTTTCTCTTGTGCCTTCAAGAGTGCAAGAAACTGACCACTGCCCCT
AGAGAGCTCTCTGAAGAGCATTCCCATGGCTACAGATTGGGTTTGGAGCC
GCTTGGGCTCACCATGGGCCAGCATTGTTTTGGATGGCCTGAGGGCAAGG
ACTGTCCCTGCTCTTCATGCATCCTGAAGATTAGCTCAGTACCTGGCACA
GAGTCGATGTTCCAGAGAGGTTTGGTGAATACAGAAGGTCCCTGTTGCAT
GGAATCCTGCAAAGCAATGTTGGTGTTACCGGAACCATAGGAAACAGCTG
ATATCCAACCATTTTTGAACCTACACAGGTGTCAGTTTCATGTGGTTCAG
CCTAATGAGAGCACCACCTTAATCCTGTTGGGTTCTCCATCTCAGCCCCA
GGGGAATGAGCAACACAGGGGAGCTAGCTGGTGGTGCCAGACTGGGGTTC
AGATCCCTGCCACATTTACATGGGCTTTGTGACACACGACGGGTCATCTA
ATGTCTCTGAACTGCAGCTTCTGCCTATGTAGAATGGGAGCAGTAACACA
GAGCATAAAGACATGATAGGGGCAAGGAGAGCAAGTGTGTAAAGCACTCA
GCTCCGGGTTTTCATGCACAAGTGTGCTGCACACTTGTGCCTGACACTTA
TTCAGTCGCACTAGCTCATCTATAGAATGGGAGGTGATACACGCCTAGCA
GGCTTGTTGTCAAAACAAACTAAGCTCACTGCAGCTGGATCAGTGATGAA
TGTCACTGTCGTCATCATTCCCACTCCACAGCCTGAGGCCTGGCCAAGTG
CTCCTGTGATGTGCCACACCAGTCCAGCAGGCAGGGGAAACATGCTTTGC
TTGGTGGGATTCAAGGTGTAGGGATGGCCCTCCCTTTACAGTCACCACCC
TGGGTTGAGAGTTTGTTCCTCCTCCTTGCTCTGGGCTGGTCTCTATGTGA
TATCTGACCAAGCCCGGTAACTGTGTCTGTTAGGTGGGATGCATGACCCA
TTCCAGCCTCTCCACAGACTCGGGAGTGCTGTCTCTATGCCAGGGTCTGA
GAATAGATACCGATACATGGGTCAGCAGCTCCTGTGGGGGTGTCACTCCA
CTGCGGAGGGAAACCAAGGCAGAAGCTGGACAAGAGTCTGAATGGCTCAG
ATTTGAATCATTCGTTTTTGGGGGGTTTTTTGTTTGTGTTTGTTTCTGTT
GTTGTTGTTGTTATTGTTTTGAGACGGAGTTTTGCTCTTGTTTCCCAGCT
GGAGTACAGTGGCGCAATCTCGGCTCACCACAACCTCCGCCTCCCGGGTT
CAAGTGATTCTCCTGCCTTAGCCTCCCGAGTAGCTGGGATTACAGGCATG
TGCCACCACGCCCGGCTAATGTTTGTATTATTAGTAGAGACGGGGTTTCT
CCATGTTGGTCAGGCTGGTCTCGAACTCCCAACCTCAGGTAATCTGCCCC
CCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCAG
CCCAAGCGTTAGTTGTGAAGGTTTATTTTGTTGCAAATCCCAGTCCTGCA
TGAAAATGGGAATGTTAGTAACCCCTGCCTCAAGGTGGGGCAGTGAAAAC
AAAAAACACCCTGGGAGTCTGCAGAGGTGTGGGCCTTTCCCATCCCATTC
TTTCTGCTACAATCAAAATGATCGTTTAAAAATGGAAATCTAGGTTGGGC
AAGGTAACTCCTACCTATAATCCTCACACTTTGGAAGGCTGAGGTGGGAG
GATCCCTTGAGCCCAGGAGTTCAAGACCATCCTGGACATGATGGCAAGCC
CCTTCTCAATAAAAAAAATTAAAAGTAACAGTAAAAAAGTAAACGCAGAA
GCCTGAGTTCCCCCTGCAGTCAGGTCAAAGCCCCCAAGTCTTACTCCAGG
CCTGCTAAGGCGTTCCCATCCCCTTCAGCTTCCCCTTTGGGCCTCTGGCT
TCTCACTGTCTGGGCTTTCGTGACAATGACCTTTAACCCCTCCGGGCAAC
AGGTGGTTCCCTCGCCTTTCAGATTCCCTCTGCCTGGGATGCTCTAGGCC
TCCATCCACCCAGACCCCTTTATGTGACTGAATCCTACTCATCCTTCGGG
GCATTCTCAGAGAAACTTCCTCCACCCACTGAGCCCCCAGCCAGTGTGGC
GTGAAGGTGGGAAGCATAGACTTGCCTGGGTTTACTCCTCCCTGCCCTCT
GCTAGCTTGCACAAGCTAGTCTTTGTACTTCATTCTCTTCATCTTTACAA
AAGAGAGGATAATAATTTCTTTCCCAGAGAATTTTGGGGTGGATTCAAGG
AGAAAGTATAGCTGACCGTCTCCAAACAGTGCCTGGCAGAAAGTAAGCAT
TCAATAACATCAGCTATTGGCCAGGCAAGGTGGCTTACTCCTATATTCCC
AGCACTTTGGGAAGCTGAGGCAGAAGGATTACTTGAGGCCAGGAATTCAA
GACCATCCTGAGCCACATACTGAGACCCCATGTCTATAAAAACCAAATTA
GGCCAGGCGTGGTGGTTCACACCTGTAATCCCAGCACTTTGGGAGGCCAA
GGCAGGTGGATCATCTGAGGTCAGGAGTTCGAGACCACCCTGGCCAATAT
GGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGTGTGATGG
GTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGA
ACCCAGGAGGTGGAGGTTGCAGTAAGCCAAGATCATGCCACTGCACTCCA
GCCTGGGCAACAAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAGAACAA
AAAAAGGAAATTAGCTGAGCATGGTGGCATGTGCCTGTAGTAGTCCCAGC
CCCTCGGGGGGAAGCTGAGGCAGGAGGATTGCTTGAGCCCAGGAGGTTGA
GGCTGCAGTGAGCCTTGATCATGCCACTGCACTTCAGCCTGGGCAACAGA
ATGAGACCCTGTCTCAGTAATAATAATATCAGCTATTGCTGTTACTGGCG
TTATTTTTTTTTTACATGCCCCAGGCCACCCAGTAGGGCACTTTTGGAAC
ACCCATTATGCTTGTAATTATTCAGTTGCCTGTCTGCCCTGCCAGATTAA
AGCTCCTTGCCTGCAGGTACTGCACGTTCTAGCTAGGCAGGGGTCTTGTT
TATTGCTTTGCTGTCCCTAGGGTGCATAGTCAGGAAACCTTGGGGCCCAG
TTCTTAGGTTCAAAGTTCAGTCAGCAATTTGCTAGATGTGTTAGCCTCAG
GTGGTACTTAACCTCAGCATCACCGTGTATAAAATGGGGGCTCCTCTAAA
CAGTGCTCAGGTTTTATGGTTGTGAGAATTAAATGAGATGGTGGATATAA
AGGATTTAGCACCATGCTTGCATGGGATGCTCTCAATATCTCGAGCACCA
CAAATACGAGGTACCATTCATAGCATCCTTCTATTTGTCCCCTCCTCCCT
GCTCCCCTGCTGTAGGCATTGAACCTGGCCACATCCCAGGTACCGTGAAC
ATCCCCTTCACAGACTTCCTGAGCCAGGAGGGGCTGGAGAAGAGCCCTGA
GGAGATCCGCCATCTGTTCCAGGAGAAGAAAGTGGACCTGTCTAAGCCAC
TGGTGGCCACGTGTGGCTCTGGCGTCACAGCCTGCCACGTGGCACTAGGG
GCCTACCTCTGCGGCAAGCCAGACGTGCCCATCTACGATGGCTCCTGGGT
GGAGTGGTACATGCGCGCCCGGCCCGAGGATGTCATCTCAGAGGGCCGGG
GGAAGACCCACTGAAGCTGGGCAGGACACAGGCGAGCTCAGGTGATGCCG
GCCACCAGCAATGCCTGGCCTGGTAGCTCCGCTTCTGCTTTCACCAAGAG
AGTGTTTCTTCACTCAACTCAGGTGGCATTTGGGGTGACATCTCAAAGGC
CAGGAATTCCGTTGACTTGTTGGCTGCCAGTAGGGGCGGGAGGAAAGGCG
GAGGCGAGCCCTGGAGGAGGGAGGCCACAACTCCGAGCTGCCCACCTGGT
GCTGAGCTGGGGCCCCGCCTCCTTTCTGTTTTATTTTTGAGGAAATAAAA
TAACCAAGTGCT
10
Supplementary Figure 5
AGGAAAGTTTCATTTGGCCGGGCGCGGTGGCTCATGCCTGTAATCCCAGC
ACTTTGGGAGGCCGAGGCAGGTGGATCACTTGAGCTCAGGAGTTTGAGAC
CAGCCTGGGCAATATCGTGAGACCCCATCTCTACAAAAATACAAATTAAC
TGGGCATCCTGTCATGCGCCTGTCATCCCAGCTACTTGAGAGGCTGAAGC
AGAAGAATCTCTTGAGCCCGGAAGGCAGAGGTTGCAGTGAGCTGAGATCG
TGCCACTGCACTCCAGCCTGAGTGACAGGAGTTAAGCCCTGTCTCAGAAA
AAAAAAAAACAAAAACCCAAAAAGTACTTAAAGTTTCATTTACTTAGCTA
GGAGAAAGACTTGGTTCTCAAAATAATACATTTTAAGATTAATTGGGTAG
AATTAGAGTTCCACCTTTATTCATTGTTGAGCAGTGATTTATATTTAGTT
ATTATATTTAGGAATAGAAAATAGAATTAAATAATTTAACTTGATTAATC
AACCAGACCAATTTTGACGTCTGGAGAACTTATAAACTCAATATGTTATA
TTTGTTTTCCTGTATTTAGGTGGAGAAATGAGAAAAAGCTTTAGTTCCAA
TTGACAGTTCAGGTCAATGTTCAATGAACATTCCTATCTTCTAAACCAGA
TTTCTCTTTTTTTTTCCTGAGACGGAGTCTCGCTCTGTAGCCTAGGCTAG
AATGTAGTGGCGTGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCA
TGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGGCACCGC
CACCACGCCTGGCTAATTTTTTGTATTTTTAGTAGAGAAGGTGTTTCACC
GTGTTAGCCAGGATGGTCTTGATCTCCTGACATCGTGATCTGTCTGCCTC
GGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCTA
AACCAGATTTCTTTAGGGCACAATTTTTTCTGGAATCTCACTCTTGTTTT
TCACAGTAATTTTAAAAACGTTTCTACTCCAATTAAGAATATATATGATG
TTATTATATATGCTTATGAAACAGATTTATGAGAAAAGTTTTTTTTAAAT
AAATTATTTAATCCTAGCTCTTTGTTATTCACCAGCTTTTTAAAAAAGTG
TTTGAAAATTATACATTCTCGACTTTGGGGATTAGTTTATTCTTGTGGCA
TCATTATAAGGCTTAATTGTGGAAAGCTAAGTACTTGCTTCTATTCCTTC
CAATGAGAGATTATAACATAAACTATTAAAATTATTCTGCTAATATACCT
AGATTTTTATTTTTAGGTCTCTGTCCATTGCTTTTAATTAAAATTATCTG
TTTTCAAATCAA
11
Supplementary Figure 6
A
B
BPNT1 3 UTR
1st ALU RC
2nd ALU RC
WT
Firefly
Renilla