Darwin%20J.%20Prockop,%20M.D.,%20Ph.D.,

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• Darwin J. Prockop, M.D., Ph.D.,
• Director, Center for Gene Therapy, Tulane
  University Health Sciences Center, New Orleans,
  LA
• ???violet39 (DXY)

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• (?? Prockop et al. PNAS 100(1) 11917-23, 2003)

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• ???????? (MSCs)?
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• ??????,???????HSCs????
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• ????,???????MSCs

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• Friedenstein,1976,??????(MSCs)??????
• ?????????110,000?100,000
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???MSCs????

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MSCs???

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• ???5????? MSCs
• ??2????

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??12??,????????2000?? (Colter, DiGirolamo,
Prockop, 2000)
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???? 1?????? 2???????? 3???,??,????????(RS
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Dickkopf-1, Wnt ?????????? ???35kDa?????????????
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??Dkk-1??MSCs?????????(????)
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Dkk-1???????
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Wnt???,Dkk-1??????hMSCs?????
Dkk-1Wnt??????????
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MSCs?????????????

• a????????????????,??????Wnt?????????Dkk-1?
• b??Dkk-1?????,????Wnt???????????Wnt-5a?,???????
• c?????????,?????????,???4-6?,???????????

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??????(4 - 6Xs)
??/??
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Dickkopf-1(Dkk-1)

• Wnt??????????
• ?????????Wnt????,???????
• ???????MSCs??
• ??????Dkk-1?????????MSC?????????

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????????MSCs??
RS???? ???? ???
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??MSCs???????????

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• NIH??430?????tulane??,??????????????????

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????SAEGFP/MSCs
???????(SAE) GFP /MSCs
( 20 )
( 40 )
?????????????????,GFP???MSCs??????????,???
?????????,???????
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3. MSCs???????????Spees et al. PNAS 2003
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??MSCs
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1??? 2?????(?)
3????? (Wnt 5a, VEGF, PDGF, BDNF, NT-3)
???MSCs?? (????)
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• (Hoffstetter et al. PNAS 2002)

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(No Transcript)
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• ??? 0/10 6.8 /- 0.4 SEM
• ??? 7/12 9.2 /- 0.4 SEM
• 
  (p0.013)
• 2????????

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hMSCs????????
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• ???????????,???????????MSCs??????
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• (???????????????????????????)

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• Prockop, D.J., C.A. Gregory and J.L. Spees.
  One strategy for cell and gene therapy
  Harnessing the power of adult stem cells to
  repair tissues.
• Proceedings of the National Academy of
  Sciences 100(1)11917-11923(2003).