ANALYSIS OF SIDE VIEWS OF BMS PROTOPLASTS

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ANALYSIS OF SIDE VIEWS OF BMS PROTOPLASTS
. A side view of a protoplast . IMAGE (2) is
identical to . IMAGE (1), except for the
. marks defining the measu- . red
dimensions. See also . the the file Video
and figure . legends.
The height (a), width (b) . and base (c)
in the Table parts (A and B on the following
page) refer to protoplast dimensions as marked on
the protoplast images. The measurements were
performed during the experiment on the captured
protoplast images using Scion Image (see
Materials and Methods). roundness the ratio
between the horizontal and vertical diameters of
the protoplast in a side view, with the vertical
extrapolated beyond the base to the continued
circular contour (h is the height of the missing
cap below the base).
- The protoplasts underwent a hypotonic
challenge from 350 mOsm to 150 mOsm. - The
above measurements in the hypotonic solutions
were performed 90 s after the hypotonic step.
- The green-highlighted cells in both Table parts
(20 - 22) are identical cells.
The contact disk significance contact disk
the area between the protoplast and the glass
see Image 1. This disc is the base also of the
missing cap of the globule. We assume c
reflects the contact disk diameter. Upon
swelling, c increased from 21.46.8 (SD) ?m in
the isotonic solution to 29.88.6 ?m in the
hypotonic solution, and the calculated surface
area of the "missing cap" of which this contact
disk is a base remained, on average, within 6
of the total calculated surface area of the
globular protoplast in either solution. Thus,
the total area available for water entry into the
protoplast would be still 94 of that of the
globule, which would lead to a negligible
underestimate (by 6) in the determination of Pf
(see also Video S-II, Supplemental Material).
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