Preparation for a paper

1
(No Transcript)
2
(No Transcript)
3
(No Transcript)
4
(No Transcript)
5
(No Transcript)
6
(No Transcript)
7
Preparation for a paper

• All notes should be complete such that we can go
  back and reference them for future writing
• We want to tell a story so including motivation
  and plans is important (take the time it will be
  worth it)
• Preparation of figures and presentation slides
  along the way will help develop our story and
  solidify our plans
• Limited time necessitate preparing our
  presentation and any writing we want to do along
  the way

8
Image preparation

• Existing rasterized images (gels, photos etc.)
  should be modified (ethically) in photoshop
• New drawings or modification of graphs and tables
  should be done in illustrator
• Save in native adobe format as well as other
  formats for web or integration into writings
• Save graphs as .ps or .pdf from graphing program
  to import into illustrator

9
More imaging

• We will try to keep a standard format for figures
  as we go so that they can be put together easily
• Keep font sizes, line weights, color matching the
  same throughout image as well as across images
• When making images for presentations save as .png
  with transparency (also save as .ai or .psd)

10
Labeling and reaction mixing

• Label each sample carefully and non-ambiguously
  before adding reagents
• Make a list of reagents for each reaction before
  starting
• check off each reagent as you add them to the
  reactions
• Double check everything is there before
  incubating or running reaction

11
Pipetting

• Make sure you have the right pipette for the
  volume needed
• When taking fluid depress button of pipette only
  to the first level of resistance
• Look at tube and tip when taking up reagent
• When ejecting liquid depress button of pipette
  all the way down remove from sample and release