PCR A license to do molecular biology

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• PCR A
  license to do molecular biology

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DNA FINGERPRINTING BY PCR
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POLYMORPHIC DNA

• Chromosomal regions that vary widely from
  individual to individual
• By examining several of these polymorphic regions
  within the DNA, one may obtain a DNA
  fingerprint

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• Can identify criminals
• Can determine paternity
• Can identify human remains
• Serve as markers for certain diseases

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• Most commonly used polymorphisms are those which
  vary in length
• FLPS-fragment length polymorphism
• When amplified by PCR referred to as AmpliFLPs

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VNTR

• Variable number of tandem repeats
• FLPs that contain specific sequences duplicated a
  variable of times

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D1S80

• Is a specific type of VNTR
• Located on chromosome 1
• Contains a 16 nucleotide sequence
• Variable repeated 16-40 times
• D1S80 locus is one VNTR used by FBI

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• An individual who is homozygous
• For the D1S80 genotype will have equal repeat
  numbers on both homologues of chromosome 1
• Yields a single PCR product seen on gel
• An individual who is heterozygous for the D1S80
  genotype will have different D1S80 repeat numbers
• Yields 2 distinct PCR products seen on gel

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