Question 1:

1
Quiz 3 Biol 302 Spring2011
20pts total
Name_______________
Question 1
Draw and Label a Two Exon Complete Gene.
10pts-labeling 14 structures correctly,
8pts-labeling 10-13 structures correctly,
5pts-labeling 6-9 structures correctly. 0pts for
less than 6 structures labeled correctly or for
any inaccuracies.
5
3
Question 2
The two differences between splicing of a
transcript and gene recombination
are 1pt_______________________________ 1pt_______
________________________
Question 3
Given the following DNA binding motif
Helix2-turn-Helix3 for a homeodomain protein.
Trp48, Phe49, Asn 51 and Arg53 are invariant
amongst almost all homeodomains in order to
retain DNA binding. However, you want to test
this idea with single amino acid substitutions.
(2pts for 3 correct) What will you change Trp48
to _____ What will you change Phe49 to
_____ What will you change Asn51 to _____ What
will you change Arg53 to _____
2
Question 4 Given the following patterns
produced in Drosophila segments starting from
1(WT)
Fill in appropriate segment identity in boxes
below.
WT
1 plus overexpression of Antp in all segments,
2pts
1 plus overexpression of Ubx in all segments,
2pts
1 plus overexpression of AbdA in all segments,
2pts
1 minusUbx
1 Minus Antp
1
-
Antp
Antp
GS
Ubx
Antp
Ubx
A1
T2
A1
AbdA
AbdA
AbdA
A2
A2
A2
AbdA
AbdA
AbdA
A2
A2
A2
AbdA
AbdA
AbdA
A2
A2
A2
AbdA
AbdA
AbdA
A2
A2
A2
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
Extra Credit 2pts The above results of segment
identity can be explained in two ways
Either the _______________________________________
______________ or
__________________________________________________
______________________