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Genetic Variation

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How well the protein works ... GAATTC versus GATTTC RFLP analysis is the detection of the change in the length of the restriction fragments as a result of these ... – PowerPoint PPT presentation

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Title: Genetic Variation


1
Genetic Variation
  • Most genes have small sequence differences
    between individuals
  • Occur every 1350 bp on average
  • Some of these polymorphisms may affect
  • How well the protein works
  • How the protein interacts with another protein or
    substrate
  • The different gene forms containing polymorphisms
    are called alleles

2
Between-population variation
  • Salamanders

3
Within-population variation
  • Hawaiian Happy-face spiders, Theridion grallator

4
RestrictionFragmentLengthPolymorphismRFLPAna
lysis
  • Some genetic polymorphisms can be identified
    by the presence or absence of a specific
    restriction endonuclease recognition siteFor
    example
  • GAATTC versus GATTTC
  • RFLP analysis is the detection of the change in
    the length of the restriction fragments as a
    result of these mutations.

5
EcoR1
EcoR1
TTCGTCGAATTCGTTATGCGAATTCTGCATAATGGTC TTCGTC
GAATTCGTTATGCTAATTCTGCATAATGGTC
EcoR1
6
(No Transcript)
7
Paternity Testing
8
Criminal cases
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