iTRAQ??

1
iTRAQ??
2
????????

• 2DE-MS/MS
• DIGE-MS/MS
• ICAT
• ????
• MudPIT
• iTRAQ
• ..

iTRAQ(??????????????)??????????,?????????????????
?
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3
iTRAQ ??????
Reporter group Mass 114 to 117
Peptide Reactive Group
Balance group Mass 31 to 28
MS fragment site
Isobaric Tag Total Mass 145
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4
iTRAQ ??????

• 4??????????????????????
• Balance group???????????
• Reporter group ???????4?????,???114?115?116?117
  
• ????????????????????????????????????

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5
????
??1
114??
??2
115??
??
????
??
??3
116??
??4
117??
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6
??

• ????????,??????
• ????????????????
• ???????????,???????
• ??????????,???????????
• ????,??????????

???????????? http//www.boyuanbio.com
7
????iTRAQTM ????
QSTARTM Elite
4800 MALDI TOF/TOFTM
Q-TRAPTM
???????????? http//www.boyuanbio.com
8
????

• Biomarker Discovery
• Signaling/Differential PTM analysis
• Genomic proteomic correlation
• Time course analysis
• Membrane protein analysis
• Lipids and phospholipids analysis

???????????? http//www.boyuanbio.com
9
Thank you!
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