Systems Biology Project

1
Systems Biology Project
1,3-Propanediol
Group2 ?? ??? ?? ??? ?? ??? ?? ???
2
Introduction of 1,3-Propanediol
3
The usage of 1,3-Propanediol
As a monomer in the synthesis of polymethylene
terephthalates and the manufacture of
polyurethanes and cyclic compounds. Important
intermediate for synthetic, it can be used as
intermediate of Vitamin H, PTT, 1,3
Dibrominepropane, 1, 3-Propanediol methyl ether,
3-chloro-1-propanol and so on. As a material
for the manufacture of carpeting and textile
fibers ?? ??,?????, ????, ???????????. (?PDO????
???????????????????????, ???????
(?????????)???????????????.
4
Find the metabolic pathways involved in the
synthesis of 1,3-Propanediol
1,3-propanediol dehydrogenase
3-hydroxypropanal
NAD
NADH
5
Find the enzymes and genes involved in these
pathway reactions
glycerol-3-phosphate dehydrogenase (NAD)
triosephosphate isomerase (TIM)
glycerol-1-phosphate phosphohydrolase
glycerol dehydratase
1,3-propanediol dehydrogenase
6
Triosephosphate isomerase (TIM), E5.3.1.1, tpiA
HSA 7167(TPI1)MMU 21991(Tpi)RNO 246267(LOC246267) 24849(Tpi1)DRE 192310(tpi1b)DME CG2171-PA(CG2171) CG2171-PB(CG2171)CEL Y17G7B.7(tpi-1)ATH At2g21170(F26H11.7) At3g55440(T22E16.100)CME CMQ172CSCE YDR050C(TPI1)AGO AGL201C(AGL201Cp)CAL orf19.6745(TPI1)SPO SPCC24B10.21(tpi)ECU ECU11_0230PFA MAL3P6.25 PF14_0378CHO Chro.10337ECO b3919(tpiA)ECJ JW3890(tpiA)ECE Z5464(tpiA)ECS ECs4844ECC c4871(tpiA)STY STY3789(tpiA)STT t3537(tpiA)STM STM4081(tpiA)YPE YPO0085(tpiA)YPK y0052(tpiA)YPM YP0089(tpiA)YPS YPTB0081(tpiA)SFL SF3997(tpiA)SFX S3750(tpiA) ECA ECA0867(tpiA) ECA4272(tpiA)PLU plu4772(tpiA) BUC BU307(tpiA)BAS BUsg297(tpiA)BAB bbp285(tpiA)WBR Wbr0496(tpiA)BFL Bfl601(tpiA)HIN HI0678(tpiA)HDU HD0762(tpiA)PMU PM1311(tpiA_1) PM1640(tpiA_2)XFA XF0303XFT PD0245(tpiA)XCC XCC2531(tpiA)XAC XAC2707(tpiA)VCH VC2670VVU VV11343VVY VV3025VPA VP0239PPR PBPRA0235PAE PA4748(tpiA)PPU PP4715(tpiA)PST PSPTO4494(tpiA)ACI ACIAD0363(tpiA)SON SO1200(tpiA)CBU CBU1450(tpiA)MCA MCA0674(tpiA)NME NMB1887NMA NMA0570(tpi)CVI CV0939(tpiA)RSO RS03629(tpiA)BMA BMA1832(tpiA)BPS BPSL1209(tpiA)BPE BP0801(tpiA)BPA BPP3426(tpiA)BBR BB3876(tpiA)NEU NE1779(cbbJ) HPY HP0194(tpia)HPJ jhp0180HHE HH1315(tpi) WSU WS0424(tpiA)CJE Cj1401c(tpiA)GSU GSU1628 DVU DVU1677(tpiA)BBA Bd1051(tpiA)DPS DP0100WOL WD0091(tpiA)MLO mll0610 mlr7275SME SMc01023(tpiA1) SMc01614(tpiA2)ATU Atu1620(tpiA)ATC AGR_C_2987BME BMEI0846 BMEII0425BMS BR1138(tpiA-1) BRA0869(tpiA-2)BJA bll4807(tpiA)RPA RPA2888(cbbJ)BHE BH05680(tpiA)BQU BQ04840(tpiA)CCR CC1893BSU BG10897(tpi)BHA BH3558(tpi)BAN BA5366(tpi)BAR GBAA5366(tpi)BAA BA_0225BAT BAS4987BCA BCE5240(tpi)BCZ BTZK4826(tpi)BTK BT9727_4816(tpiA)OIH OB2436(tpi)SAU SA0729(tpi)SAV SAV0774(tpi) SAM MW0736(tpi)SAR SAR0830(tpiA)SAS SAS0740SEP SE0559 LMO lmo0346 lmo2457(tpi)LMF LMOf2365_0366(tpiA-1) LMOf2365_2430(tpiA-2)LIN lin0364 lin2551(tpi)LLA L0006(tpiA)SPY SPy0613(tpi)SPM spyM18_0680(tpiA)SPG SpyM3_0433(tpi)SPS SPs1422SPA M6_Spy0530SPN SP1574SPR spr1432(tpi)SAG SAG0763(tpiA)SAN gbs0783SMU SMU.715LPL lp_0791(tpiA)LJO LJ0874EFA EF1962(tpiA)CAC CAC0711(tpi)CPE CPE1302(tpiA)CTC CTC00380TTE TTE1760(tpiA)MGE MG431(tpia)MPN C12_orf244(tim)MPU MYPU_4580(tim)MPE MYPE3730(tpiA)MGA MGA_0357(tpiA)MMY MSC_0823(tpi) MMO MMOB4190(tpiA)MHY mhp277(tpiA)UUR UU181(tpiA) POY PAM170(tpiA)MFL Mfl504MTU Rv1438(tpi) MTC MT1482MBO Mb1473(tpi)MLE ML0572(tpi)MPA MAP1166(tpi) CGL NCgl1524(Cgl1586)CEF CE1704CDI DIP1308(tpiA)SCO SCO1945(SCC54.05c) SMA SAV6298(tpi)TWH TW302(tpi)TWS TW470(tpiA)LXX Lxx11540(tpiA)PAC PPA0818BLO BL0708(tpi)STH STH244FNU FN1366RBA RB7095(tpiA)CTR CT328(tpiS)CMU TC0604CPN CPn1063CPA CP0786CPJ CPj1063(tpiS)CPT CpB1106CCA CCA00308(tpiA)PCU pc0801(tpiA)BBU BB0055(tpiA)TPA TP0537 TDE TDE1236(tpiA)LIL LA1696(tpiA)LIC LIC12094(tpiA)BTH BT3929PGI PG0623(tpiA)SYN slr0783(tpiA)SYW SYNW0823(tpiA)SYC syc0290_d(tpi)TEL tlr0966(tpi)GVI gll1040(tpi)ANA alr4385PMA Pro0901(tpi)PMM PMM0829(tpi)PMT PMT0817(tpi)CTE CT1444(tpiA)DRA DR1339TTH TTC0581AAE aq_360(timA)TMA TM0689MJA MJ1528(tpiA)MMP MMP0687(tpiA)MAC MA4607(tpi)MMA MM1278MTH MTH1041MKA MK1664(tpiA)AFU AF1304(tpiA)HAL VNG1027G(tpiA)HMA rrnAC0537(tpiA)TAC Ta0313TVO TVG1351870PTO PTO0348PHO PH1884PAB PAB1208(tpiA)PFU PF1920APE APE1538SSO SSO2592(tpiA)STO ST2030PAI PAE1501(tpiA)
7
glycerol-3-phosphate dehydrogenase (NAD),
E1.1.1.8, GPD1
HSA 2819(GPD1)MMU 14555(Gpd1)RNO
60666(Gpd3)DME CG3215-PA(CG3215)
CG9042-PA(CG9042) CG9042-PB(CG9042)     CG9042-PC
(CG9042)CEL F47G4.3 K11H3.1aATH
At5g40610(MNF13.130)CME CMD113CSCE
YDL022W(GPD1) YOL059W(GPD2)AGO
ADR311C(ADR311Cp)CAL orf19.1756(GPD2)
orf19.691(GPD1)SPO SPAC23D3.04c(gpd2)
SPBC215.05(gpd1)ECU ECU05_0270PFA PF11_0157
PFL0780wCHO Chro.20028WOL WD0731(gpsA)
8
glycerol dehydratase, E4.2.1.30
STY STY2245 (pduC) STY2246 (pduD) STY2247
(pduE)STT t0832 (pduE) t0833 (pduD) t0834
(pduC)STM STM2040 (pduC) STM2041 (pduD) STM2042
(pduE)MLO mll6722LMO lmo1153 lmo1154
lmo1155LMF LMOf2365_1161 (pduC) LMOf2365_1162
(pduD) LMOf2365_1163 (pduE)LIN lin1117 lin1118
lin1119CPE CPE0929 CPE0930 CPE0931
9
1,3-propanediol dehydrogenase, E1.1.1.202
BCE BC2176LPL lp_3051 (dhaT)CTC
CTC00506LIL LA2996 (dhaT)LIC LIC11070AAE
aq_1145 (dhaT)
10
Co-factor manipulation
Coenzyme B12-inact
glycerol dehydratase
Coenzyme B12-dependent enzyme
reactivase
Coenzyme B12-inact
glycerol dehydratase
Coenzyme B12
glycerol dehydratase
11
Phosphotransferase system (PTS)
Tpi Triosephosphate isomerase DAR1 glycerol
3-phophate dehydrogenase GPP2 glycerol
3-phophate phosphatase DhaB glycerol
dehydratase DhaT 1,3-propenediol dehydratase
12
acrolein
Ethylene oxide
H2O, H2
CO2, H2
energy-intensive and required expensive starting
materials
Shell process
Degussa/Dupont process
H2
1,3-propanediol
Dupont/Genencor process
Yeast/ E. coli
Two-stage production
glucose
glucose
glycerol
PDO
Yeast
E. coli
13
(No Transcript)
14
Is E. Coli suitable for biosynthesis of
1,3-Propanediol
Yes!
Reason Well-studied organism Suitable for
genetic engineering Large-scale production
(production rate, life cycle)
Goal develop one step system for 1,3-propanediol
production
Our Strategy Search for a most suitable E. coli
strain ex. K12 Key genes co-express ex. In the
same operon Promoter induced by glucose ex.
(COX)-2, GLP-1 , SGLT1
Glucose-induced promoter
15
PDO production (cont.)
16
Thanks for your attention