Instructor:%20Colleen%20Malone

1
Welcome To Biotechnology
Instructor Colleen Malone Office Hours After
school by appointment only. E-mail Address
cmalone_at_conejo.k12.ca.us (email is the
best way to contact me) Telephone [phone]
ext. 1307 Website http//newburyparkhighschool.ne
t/malone/

2
My Website
3
Who is Mrs. Malone?

• Careers
• Texas AM Health Science Center
• UCLA
• Amgen
• Newbury Park High School

4
Who is Mrs. Malone?

• Classes
• Biology-CP
• Biology-H
• Marine Science-CP
• Biotechnology-CP

5
Daily Agenda

• Warm Up (to review or introduce new material)
• Introduction/Demonstration/Current Events
• Lecture and Classroom Discussion
• Laboratory Work
• Audio Visual Connection and Summary

6
Subterm 1 Units of Instruction

• Introduction to biotechnology
• Biology review
• -(biochemistry, cells, DNA structure/replication
  protein structure/synthesis)
• Chemistry review
• -molarity, making solutions, concentrations
• Techniques Micropipeting, Gel electrophoresis
  (agarose gels), Growing bacteria (LB agar plates/
  LB broth)

7
Gel Electrophoresis
8
Bacterial Cell Cultures
9
Subterm 2 Units of Instruction

• Amgen-Bruce Wallace Program
• OR
• Coastal Marine Biolabs (DNA Barcoding Project)
• Techniques DNA extraction, Bacterial
  transformation, Polymerase Chain Reaction, DNA
  sequencing

10
DNA Extraction/Purification
11
Bacterial Transformation
Special genes from fireflies, for instance, have
been inserted into cells using the crown gall
bacterium to produce glowing plants
12
PCR
13
DNA Sequencing
14
Grading Scale

• Grading will be based on the following
  percentages using category weights.

Classwork/Homework LATE Passes? 10
Tests/Quizzes 55
Labs / Projects (includes lab notebook) 25
Final Exam/Project 10
15
Grading Scale

• Course grades will be determined by percent
  weights in several categories. The final grade
  will be based on the following percentages
• 93 - 100 A 70-72 C-
• 90-92 A- 63-69 D
• 83 - 89 B 60-62 D-
• 80-82 B- 0-59 F
  73-79 C

16
Homework

• Homework will be assigned on a regular basis
  Monday through Friday.
• Even if a homework assignment has not been
  given, time should be spent each night reviewing
  what we did in class that day.
• Students are responsible for following the
  formal procedure for make-up work after an
  absence.

17
Skills Your Panther Will Learn in Biology

• Reading scientific papers
• Writing scientific papers
• Organization
• Meeting deadlines
• Following directions
• Critical thinking
• Presenting data in public

18
What You Can Do To Help...

• Take an interest
• Have students do homework before social time /
  computer time
• Encourage study sessions
• Check day planner for important dates
• Check grades on my website (Zangle)
• http//newburyparkhighschool.net/malone/
• E-mail me with questions and/or concerns