Laboratory of Bioenergetics

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Laboratory of Bioenergetics

• main laboratory 3.2.11, 3.2.11a
• climatic chamber 3.2.11
• animal rooms 3.0.xx
• administrator Pawel Koteja
• Evolutionary Physiology Research Team

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Respirometric system Sable TR3
Main location 3.2.11

• oxygen analyzer - Oxzilla
• - 2-channel
• - 0-100, res. 0.001
• CO2 analyzer - CA2A
• - 0-10, res. 0.0001
• continuous measurement on 1 or 2 ch.
• sequential on 8-16 ch.
• open flow-through or semi-closed system
• from microorganism cultures (as Microoxymax) to
  large animals
• a small climatic chamber
• mobile

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Respirometric system Sable TR3
Main location 3.2.11
Example of an open-flow setup measurement of the
maximum exercise-induced rate of aerobic
metabolism
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Respirometric system Sable TR3
Main location 3.2.11
Example of an open-flow setup measurement of the
maximum exercise-induced rate of aerobic
metabolism
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More devices for respirometric measurements

• O2 analyzer Applied Electrochemistry (AMETEK)
  S3/AII
• 2-channel, 0-100, res. 0.001, very
  fast, stable, lab. use only
• 2 x O2 Sable analyzer FC-10A
• 1-channel 0-100, res. 0.001, slow, not
  stable, mobile, 12V
• CO2 analyzer Sable CA10A
• 1-channel, 0-10, res. 0.0001, fast,
  stable, mobile, 12V
• CO2 and H2O analyzer LiCor LI-840A
• Low cost "two in one", mobile,
  parameters similar to CA10A
• Several pumps, mass flow controllers, channel
  multiplexers, air driers, etc.

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Motorized treadmillfor respirometric measurements

• mouse-sized animals
• speed up to 6 km/h
• computer controlled speed regulation
• manual angle control
• waterproof

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Activity detectors passive infrared
Custom made 3M Mouse Motion Monitor

• 64 sensors
• expandable
• elastic system of data acquisition
• time resolution 0.01 sec.
• mobile animal rooms, climatic chambers

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Activity detectors - mechanical
Sable MAD- 1 ("seismographs")

• 8 sensors
• elastic system of data acquisition
• time resolution 0.01 sec.
• mobile animal rooms, climatic chambers

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Video system for behavioral analyses
Noldus Ethovision

• video cameras, multichannel recorders
• analog-digital converter ("frame grabber")
• video analysis system
• main result path of animal movement
• detection of a few individuals in one arena
• detection in several arenas (with one individual
  in each)
• several characteristics of movement speed, time
  in particular zones, "meandering"
• automated trials for standard behavioral tests
• open field
• Morris water maze
• multi-arm labyrinths

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Automatic extraction apparatusBüchi B-811

• 4-positions
• temperature regulation up to 150C
• extraction in neutral gas
• automated
• phases of the process (extraction, rinsing,
  drying)
• various types of extraction
• programmable sequences

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Automatic extraction apparatusBüchi B-811
Types of process
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Hydrolysis apparatus Büchi B-811

• 4 positions
• vials compatible with the extraction apparatus