? ? ? ? ? ? ? Molecular Pathology Lab

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? ? ? ? ? ? ?Molecular Pathology Lab

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The development of rational approaches to the
diagnosis and treatment of cancer is dependent on
the understanding of the mechanism that underlie
the formation and progression of tumor
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Traditional pathologyMolecular pathology
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Traditional pathology 1. Frozen section 2. Wax
embedded tissue Hematoxylin-eosin
stain Special stain silver stain
immunostain vessel, estrogen receptors

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Advances in molecular biologyGenetic
analysesComparative genomic hybridization(CGH)
Fluorescence in situ hybridization (FISH)Gene
expression transcriptionGenomic
fingerprintingcDNA and oligonucleotide
arraysSerial analysis of gene expressionDifferen
tial display or representational difference
analysisGene expression translation2-D gels,
HPLC, Mass spectrometry, tissue array,
microdissection and laser microscopy
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Molecular pathology (1) Human genome
project 1. novel genes 2. known genes
amplification, mutation, deletion
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Molecular pathology (2) Human genome
project Studies of mRNA can determine the
expression profile of a large number of
transcripts.
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Molecular pathology (3) Human genome
project Proteomic methodologies can identify
quantitative and qualitative protein changes
in tumors, and to examine
posttranslational modifications such as
phosphorylation of proteins.
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Loss of annexin 1 correlates with early onset of
tumorigenesis in esophageal and prostate
carcinoma. Paweletz et al. Cancer Res 606293,
2000
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References1. Going and Gusterson Molecular
pathology and future development. Eur J Cancer,
351895-1904, 1999. 2. Sirivatanauksorn et al.
Laser-assisted microdissection applications in
molecular biology. J Pathology 189 150-154,
1999.