target

1
I-SceI
I-SceI
I-CreI
I-SceI
mini-white
donor
mini-white
FRT
FRT
donor
FRT
FRT
donor
FLP, I-SceI
FLP, I-SceI
target
target
integration
replacement
sequences from donor
2
I-CreI
SSA
MMR
3
w Pdonor
w P70FLP P70I-SceI
heat shock
Pdonor
P70FLP P70I-SceI
w P70FLP10
w



screen progeny for non-mosaic colored eyes
4
w P70I-CreI
w Dp(33)Target, w
mild heat shock
Dp(33)Target, w
w P70I-CreI
w
Sb
screen for Sb sons with white eyes