GAS CHROMATOGRAPHY

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GAS CHROMATOGRAPHY

• Mr. Maywan Hariono

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• One of chromatography technique that use a gas as
  a carrier (mobile phase)
• The separation is based on the volatility of
  sample
• Same with HPLC, GC is one of advance
  chromatography method and use Rt as a separation
  parameter

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Phase??

• Stationary phase octadecyl silane, octasilane,
  aminosilane
• Mobile phase (gas) He, N2, H2, Ar

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The principle and instrumentation of GC
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Principles

• The volatile sample in the liquid matter is
  injected to the column.
• The temperature of column is setted in which
  sample can be vaporized.
• The gas is pumped and then it carries the sample
  (gas) through the column.
• The sample which has higher boiling point/melting
  point will be retained by column, in contrast the
  lower one will be carried through the column
  faster.
• Means the higher b.p/m.p wil be lower the Rt and
  vice versa

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Principles

• The information from GC machine will be
  translated to the graph by software

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Komponen2 GC

1. syringe
2. Injection port
3. column
4. GC oven
5. detector
6. Carrier gas
7. recorder

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GC Component

1. Syringe 5-10 microliter in volume

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GC Component

• b. Injection port

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GC Component

• c. Column

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Factors influence the GC separation performance

• i.Boiling point/melting point
• ii. Column temperature
• iii. Column length
• iv. The speed of gas stream
• v. Internal diameter of column

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GC APPLICATIONS

• 1. Pharmaceutical industry
• 2. Herbal medicine industry
• 3. Environmental analysis
• 4. Cosmetics analysis

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adavntages

• Precise and accurate
• Sensitive

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Disadvantages GC

• Time consuming
• Only volatile sample can be determined using GC
• iii. The sample is damaged after running, can not
  be recollected.

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Rujukan

• 1.David G Watson(2005). Pharmaceutical Analysis.
  Second edition. Pp 236-265
• 2.http//www.en.wikipedia.org/wiki/Gas-chromatogra
  phy.html
• 3.http//orgchem.colorado.edu/hndbksupport/GC.html