Prepare a Wet Mount

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Prepare a Wet Mount
CAPT Mike Hughey, MC, USNR
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First, a Video
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Obtain a Specimen

• Spatula
• Cotton-tipped Applicator
• Obtain directly or from inside curve of speculum

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Place on Microscope Slide

• Use very small amount
• Too much makes the slide too thick and unreadable

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Add NaCl and KOH

• One drop
• Mix well
• Two slides vs. one slide?

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Add Coverslip

• Remove excess fluid so the coverslip is stable.
• Heat the KOH slide?

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Microscopic Examination

• Start with low power
• Rarely need to go above medium power
• Focus up and down through the plane of the cells

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Yeast
Single branch
Tangle of threads
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Watch this video
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Trichomonas

• About the same size as a white blood cell
• Smaller than a vaginal epithelial cell
• Violent motion

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Watch this video
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Bacterial Vaginosis

• Clue cells
• Vaginal epithelial cells studded with bacteria
• Smudged, granular appearance
• Indistinct borders

Clue Cell
Normal Vaginal Epithelial Cell
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Clue Cells
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