Electron microscopy of influenza virus

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• ??? ????????
• 2005/10/30

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Electron microscopy of influenza virus
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Structure and Gene Fragments of the Influenza
Virus
H1-16 N1-9
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?????????

• H5, H7???????????? (Highly pathogenic avian
  influenza, HPAI),?????????????
• ????H5, H7????HPAI?

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Susceptibility of avian and mammalian species to
H5N1 (A/Ck/HK220/97)

• Mortality Birds
• 8/8 Turkeys, chickens, quail, guinea fowl,
• pheasants, Zebra finches
• 6/8 Partridges, H finches, budgerigars,
• 0/7 H sparrows (death in Italy, Ab in
• Tainan)
• 0/8 geese, ducks, gulls, starlings, pigeons,
• rats, rabbits AD47956

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High susceptibility
Some H5N1 in 2002
2005
Low susceptibility
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??AI???-??????

• 2000-2001???22???90?????????
• ??????? (HI)????H6????????3.5 (127/3650)?
• ?4-5?????????????,???????RT-PCR??????????311??????
  ???AI?????
• (???????, 2001)

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???????????

• ??????????H5?H7??HPAI?LPAI ??????,??????????,???21
  ????????????(Panigraphy et al., 1996)
• ?????H5N9????????????,?????????,?????????????????,
  ???????? (Narayan et al., 1969)
• ?????????19???,??1???,?2??????????,1????????
  (Slemons and Easterday, 1972)
• ????1997??????H5N1????????,??????????,????????
  (Laura et al., 2002 Perkins and Swayne, 2002)
• ????????????????,???????????

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?????????????
??????
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2003?????????????E-319???
???????????
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??????
???????????20-30?
??????
??????
??????
???????????
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????????

• ??????????????H5?H7???????,???????????
• ??????????????,?RT-PCR??????,??????????????

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?RT-PCR??????????????????
20 imported lots Random sampling 27 samples in
one detection including 3 sites from 3 lumps
from 3 boxes in one lot.
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Diagnosis of AI by Multiplex PCR
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Egg Inoculation
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???????????????????? (receptor) ??

• Avian H1 H 15, N1 N9
• a-2,3 linkage
• Human H1, H2, H3 N1- N2
• a-2,6 linkage
• Avian H1?human H1

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• ?????????????,????????,??????????,????????

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Thank You!
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• ??????????,???????????
• ????????????

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Isolation and characterisation of H5N1 and H9N2
influenza viruses from pigs in China (2002-2003)

• 4.7 and 8.2 per cent of serum samples collected
  from Guangdong and Fujian provinces were H5
  subtype influenza positive. 6 H9N2 and 2 H5N1
  subtype influenza viruses were isolated from the
  samples that were collected during 2001-2003.
  Sequence analysis confirmed that these viruses
  are closely related to the H9N2 or H5N1 subtype
  avian influenza viruses that have been isolated
  in China. This study confirms the H9N2 influenza
  infection in pig flocks in China, and also is the
  1st report of the emergence of the H5N1 influenza
  virus in the pig species. Therefore, for both the
  veterinary and public health sectors, urgent
  attention should be paid to the pandemic
  preparation for these 2 influenza subtypes.

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????????? (reassort)

• ?????? (Orthomyxoviridae),A????????
• A???????????
• 16?HA??,9?NA???
• Reassortment
• H5N2H6N1 H5N1, H6N2
• HA, NA 16x9144 ???
• 6 internal genes

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Postulated evolution of the influenza A viruses
currently circulating in humans
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(No Transcript)