Elementorganic Toxins

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Lecture 8

• Elementorganic Toxins

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Determination Graphite furnace atomic
absorption Cold Vapor Atomic absorption and
Fluorescence ICP-MS HPLC-ICP-MS GC-MS GC-ECD
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Electrodeless Discharge Lamps Sn, Cd, Hg, Zn,
Pb, As, Sb, Bi, Tl, In Se, Te, Rb, and Cs
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Digestion Procedure
0.1-0.3g
Sample
KMnO4
HNO3
NH3OH
_at_85oC
30 min
Overnight
To MHS-15
To convert organic mercury into Hg2
To remove MnO4- ion
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PerkinElmer MHS-15 Mercury Analyzer
N2
To cuvette
SnCl2
Sample compartment
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Thermo Jarrel Ash Smith-Hieftje 11 AAS
Hollow cathode Hg lamp
Detector
Quarz cuvette
Monochromator
253.6 nm
Hg vapor
Data processing
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ECD detector
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ICP detector
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sarin
warfare agent
GC
Malathion
Malaoxon
Organophosphorus Pesticides
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2. hexamethylphosphoramide 3. mevinphos 4.
trichlorfon 5. TEPP 6. thionazin (IS) 7.
demeton-O 8. ethoprop 9. tributyl phosphate
(SS) 10. dicrotophos 11. monocrotophos 12.
naled 13. sulfotepp 14. phorate 15.
dimethoate 16. demeton-S 17. dioxathion 18.
terbufos 19. fonophos 20. phosphamidon isomer 21.
diazinon 22. disulfoton 23. phosphamidon 24.
dichlorofenthion 25. parathion-methyl 26.
chlorpyrifos methyl 27. ronnel 28.
fenitrothion 29. malathion
30. fenthion 31. aspon 32. parathion-ethyl 33.
chlorpyrifos 34. trichloronate 35.
chlorfenvinphos 36. merphos 37. crotoxyphos 38.
stirofos 39. tokuthion 40. merphos oxidation
product 41. fensulfothion 42. famphur 43.
ethion 44. bolstar 45. carbophenothion 46.
triphenyl phosphate (IS) 47. phosmet 48. EPN 49.
azinphos-methyl 50. leptophos 51. tri-o-cresyl
phosphate 52. azinphos-ethyl 53. coumaphos
Organophosphorus Pesticides
Oven temp. 100C to 180C _at_ 10C/min. (hold 2
min.), to 300C _at_ 18C/min. Inj. / det. temp.
250C / 280C FPD Solvent hexane
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2,4-Dichlorophenoxyacetic acid (2,4-D)
2,4,5-Trichlorophenoxyacetic acid (2,4,5-T)
1. 1,4,7,8-TCDD 2. 2,3,7,8-TCDD 3.
1,2,3,4-TCDD 4. 1,2,3,7 5. 1,2,3,8-TCDD 6.
1,2,7,8-TCDD
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
GC 2.0µL splitless injection of TCDD
isomers, approximately 0.5ng each
isomer Splitless hold 0.45 min ECD detector